VAC14_ARATH
ID VAC14_ARATH Reviewed; 743 AA.
AC Q9ZU97; Q0WVB5; Q8RXM7;
DT 03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Protein VAC14 homolog;
GN Name=VAC14; OrderedLocusNames=At2g01690; ORFNames=T8O11.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 461-743.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 461-743.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
CC -!- FUNCTION: The PI(3,5)P2 regulatory complex regulates both the synthesis
CC and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
CC Regulates the synthesis of PtdIns(3,5)P2 by positive activation of FAB1
CC and by controlling SAC/FIG4 localization (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the PI(3,5)P2 regulatory complex at least
CC composed of ATG18, SAC/FIG4, FAB1 and VAC14. VAC14 nucleates the
CC assembly of the complex and serves as a scaffold (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Vacuole membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9ZU97-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the VAC14 family. {ECO:0000305}.
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DR EMBL; AC006069; AAD12702.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC05485.1; -; Genomic_DNA.
DR EMBL; AY080803; AAL87284.1; -; mRNA.
DR EMBL; AK226840; BAE98933.1; -; mRNA.
DR PIR; H84427; H84427.
DR RefSeq; NP_565275.1; NM_126230.3. [Q9ZU97-1]
DR AlphaFoldDB; Q9ZU97; -.
DR SMR; Q9ZU97; -.
DR BioGRID; 101; 2.
DR STRING; 3702.AT2G01690.2; -.
DR iPTMnet; Q9ZU97; -.
DR PaxDb; Q9ZU97; -.
DR PRIDE; Q9ZU97; -.
DR ProteomicsDB; 228547; -. [Q9ZU97-1]
DR EnsemblPlants; AT2G01690.1; AT2G01690.1; AT2G01690. [Q9ZU97-1]
DR GeneID; 814698; -.
DR Gramene; AT2G01690.1; AT2G01690.1; AT2G01690. [Q9ZU97-1]
DR KEGG; ath:AT2G01690; -.
DR Araport; AT2G01690; -.
DR eggNOG; KOG0212; Eukaryota.
DR InParanoid; Q9ZU97; -.
DR PhylomeDB; Q9ZU97; -.
DR PRO; PR:Q9ZU97; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZU97; baseline and differential.
DR Genevisible; Q9ZU97; AT.
DR GO; GO:0010008; C:endosome membrane; IBA:GO_Central.
DR GO; GO:0000306; C:extrinsic component of vacuolar membrane; IBA:GO_Central.
DR GO; GO:0070772; C:PAS complex; IBA:GO_Central.
DR GO; GO:0006661; P:phosphatidylinositol biosynthetic process; IBA:GO_Central.
DR GO; GO:0033674; P:positive regulation of kinase activity; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR021133; HEAT_type_2.
DR InterPro; IPR026825; Vac14.
DR InterPro; IPR021841; VAC14_Fig4p-bd.
DR PANTHER; PTHR16023; PTHR16023; 1.
DR Pfam; PF11916; Vac14_Fig4_bd; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50077; HEAT_REPEAT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Membrane; Reference proteome; Repeat;
KW Vacuole.
FT CHAIN 1..743
FT /note="Protein VAC14 homolog"
FT /id="PRO_0000421977"
FT REPEAT 45..83
FT /note="HEAT 1"
FT REPEAT 87..124
FT /note="HEAT 2"
FT REPEAT 128..165
FT /note="HEAT 3"
FT REPEAT 169..206
FT /note="HEAT 4"
FT REPEAT 210..247
FT /note="HEAT 5"
FT REPEAT 251..288
FT /note="HEAT 6"
FT REPEAT 292..329
FT /note="HEAT 7"
FT REPEAT 334..371
FT /note="HEAT 8"
FT REPEAT 375..412
FT /note="HEAT 9"
FT REPEAT 414..448
FT /note="HEAT 10"
FT REGION 624..647
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 670..743
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 670..687
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 697..743
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 696
FT /note="E -> EQ (in Ref. 3; AAL87284)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 743 AA; 83912 MW; 2200742856CA3BFC CRC64;
MSDALSAIPA AVHRNLSDKL YEKRKNAALE LENIVKNLTS SGDHDKISKV IEMLIKEFAK
SPQANHRKGG LIGLAAVTVG LSTEAAQYLE QIVPPVINSF SDQDSRVRYY ACEALYNIAK
VVRGDFIIFF NKIFDALCKL SADSDANVQS AAHLLDRLVK DIVTESDQFS IEEFIPLLKE
RMNVLNPYVR QFLVGWITVL DSVPDIDMLG FLPDFLDGLF NMLSDSSHEI RQQADSALSE
FLQEIKNSPS VDYGRMAEIL VQRAASPDEF TRLTAITWIN EFVKLGGDQL VRYYADILGA
ILPCISDKEE KIRVVARETN EELRSIHVEP SDGFDVGAIL SVARRQLSSE FEATRIEALN
WISTLLNKHR TEVLCFLNDI FDTLLKALSD SSDDVVLLVL EVHAGVAKDP QHFRQLIVFL
VHNFRADNSL LERRGALIVR RMCVLLDAER VYRELSTILE GEDNLDFAST MVQALNLILL
TSPELSKLRE LLKGSLVNRE GKELFVALYT SWCHSPMAII SLCLLAQAYQ HASVVIQSLV
EEDINVKFLV QLDKLIRLLE TPIFTYLRLQ LLEPGRYTWL LKTLYGLLML LPQQSAAFKI
LRTRLKTVPT YSFSTGNQIG RATSGVPFSQ YKHQNEDGDL EDDNINSSHQ GINFAVRLQQ
FENVQNLHRG QARTRVNYSY HSSSSSTSKE VRRSEEQQQQ QQQQQQQQQQ QQRPPPSSTS
SSVADNNRPP SRTSRKGPGQ LQL
