VAC14_SCHPO
ID VAC14_SCHPO Reviewed; 811 AA.
AC P87145;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 25-MAY-2022, entry version 139.
DE RecName: Full=Protein VAC14 homolog;
GN ORFNames=SPBC25H2.03;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: The PI(3,5)P2 regulatory complex regulates both the synthesis
CC and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
CC Regulates the synthesis of PtdIns(3,5)P2 by positive activation of FAB1
CC and by controlling FIG4 localization (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the PI(3,5)P2 regulatory complex, composed of
CC ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the
CC complex and serves as a scaffold (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Vacuole membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VAC14 family. {ECO:0000305}.
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DR EMBL; CU329671; CAB08779.1; -; Genomic_DNA.
DR PIR; T39998; T39998.
DR RefSeq; NP_596363.1; NM_001022284.2.
DR AlphaFoldDB; P87145; -.
DR BioGRID; 277017; 25.
DR STRING; 4896.SPBC25H2.03.1; -.
DR MaxQB; P87145; -.
DR PaxDb; P87145; -.
DR PRIDE; P87145; -.
DR EnsemblFungi; SPBC25H2.03.1; SPBC25H2.03.1:pep; SPBC25H2.03.
DR GeneID; 2540489; -.
DR KEGG; spo:SPBC25H2.03; -.
DR PomBase; SPBC25H2.03; -.
DR VEuPathDB; FungiDB:SPBC25H2.03; -.
DR eggNOG; KOG0212; Eukaryota.
DR HOGENOM; CLU_007740_0_0_1; -.
DR InParanoid; P87145; -.
DR OMA; TYLRMQL; -.
DR PhylomeDB; P87145; -.
DR PRO; PR:P87145; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0010008; C:endosome membrane; IBA:GO_Central.
DR GO; GO:0000306; C:extrinsic component of vacuolar membrane; IBA:GO_Central.
DR GO; GO:0000329; C:fungal-type vacuole membrane; ISO:PomBase.
DR GO; GO:0070772; C:PAS complex; ISO:PomBase.
DR GO; GO:0006661; P:phosphatidylinositol biosynthetic process; ISO:PomBase.
DR GO; GO:0033674; P:positive regulation of kinase activity; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR026825; Vac14.
DR InterPro; IPR021841; VAC14_Fig4p-bd.
DR PANTHER; PTHR16023; PTHR16023; 1.
DR Pfam; PF11916; Vac14_Fig4_bd; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Membrane; Reference proteome; Repeat; Vacuole.
FT CHAIN 1..811
FT /note="Protein VAC14 homolog"
FT /id="PRO_0000116490"
FT REPEAT 81..119
FT /note="HEAT 1"
FT REPEAT 122..160
FT /note="HEAT 2"
FT REPEAT 240..278
FT /note="HEAT 3"
FT REPEAT 334..372
FT /note="HEAT 4"
FT REPEAT 375..412
FT /note="HEAT 5"
FT REPEAT 431..469
FT /note="HEAT 6"
FT REPEAT 472..510
FT /note="HEAT 7"
FT REGION 775..811
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 775..797
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 811 AA; 92453 MW; F2D49FFE4BEB6B70 CRC64;
MDNLLVRGLT HKLYDKRKAT AYELERVVKG YLENDETEKI RAVISQLAND FVYSPARGPN
ATFGGLIGLA AVAIALGPKI DSYMESILLP VLYCFNDSDS KIRYYACESM YNIGKVAKGE
VFRYFNLIFD VLCKLFADTE ITVKNGAELL DRLIKDIVMQ QAATYMSSAE DIKNFKEGPV
SSSIQDVPVM STEQPRMHTF SLSELVPLLS ERLYVINPNT RMFLVSWIRL LDSIPDLEFI
SYLPFLLDGL MNYLSDPNES IRIVTSNCLY DFLREIQKIA KVKYHILQRD EESEPDFFDS
MVRRNMSDAE LKEISDYVES SLRDGSFILE AHIQIDYKRI LEIIIDHLGS SVPLIQEKAL
KWLFEFIYIA PKDVLLQIPK VLENLLPLMS NDENMRQSAK DLSQNLVILV SKIMDIEFSG
SETNNKDNSL SVDFRSLIEV LQKLLSNDNE ETRLCALEWV LLLQRRTGGK LINMHDPIFQ
TLLLQLSDPS DLVVSRTLEL LAHIAISHKS VNLVPFLKSL LQMFAEDRKF LNSRGNLIIR
QLCNYIEGER VYTSFAGILE TEENLELASI MVEVLNNNLF TAPELYDLRK KLKQSAPKLQ
NIFTTLYTAW CHNSIAVFSL CLLSQNYEHA ANLLSVFAEI EFNIDMLIQL DKLVQLIESP
VFTYMRLQLL EPEKYPYLHK ALYGILMLLP QSSAFRTLRD RLQCSSTPRT NTILANERLP
RSRRDDPYWT DLLERLKAVQ LSHQNNYREP IRATRLALAG ALPSTPTATT ISTTTSASGI
TTTASNSRDS FITRLPPTAA LSTGARKKPK Q
