ID   VAC8_CRYNB              Reviewed;         630 AA.
AC   P0CM61; Q560A4; Q5KPA7;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Vacuolar protein 8;
GN   Name=VAC8; OrderedLocusNames=CNBA3360;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Functions in both vacuole inheritance and protein targeting
CC       from the cytoplasm to vacuole. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Lipid-anchor
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the beta-catenin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAL23689.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AAEY01000001; EAL23689.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_778336.1; XM_773243.1.
DR   AlphaFoldDB; P0CM61; -.
DR   SMR; P0CM61; -.
DR   EnsemblFungi; AAW40867; AAW40867; CNA03500.
DR   EnsemblFungi; EAL23689; EAL23689; CNBA3360.
DR   GeneID; 4933594; -.
DR   KEGG; cnb:CNBA3360; -.
DR   HOGENOM; CLU_021483_0_0_1; -.
DR   Proteomes; UP000001435; Chromosome 1.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043495; F:protein-membrane adaptor activity; IEA:InterPro.
DR   GO; GO:0071562; P:nucleus-vacuole junction assembly; IEA:InterPro.
DR   Gene3D; 1.25.10.10; -; 3.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR000225; Armadillo.
DR   InterPro; IPR045156; Vac8.
DR   PANTHER; PTHR47249; PTHR47249; 1.
DR   Pfam; PF00514; Arm; 6.
DR   SMART; SM00185; ARM; 9.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS50176; ARM_REPEAT; 7.
PE   3: Inferred from homology;
KW   Lipoprotein; Membrane; Myristate; Repeat; Vacuole.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..630
FT                   /note="Vacuolar protein 8"
FT                   /id="PRO_0000410033"
FT   REPEAT          74..115
FT                   /note="ARM 1"
FT   REPEAT          117..156
FT                   /note="ARM 2"
FT   REPEAT          158..197
FT                   /note="ARM 3"
FT   REPEAT          199..238
FT                   /note="ARM 4"
FT   REPEAT          242..281
FT                   /note="ARM 5"
FT   REPEAT          283..322
FT                   /note="ARM 6"
FT   REPEAT          324..364
FT                   /note="ARM 7"
FT   REPEAT          408..447
FT                   /note="ARM 8"
FT   REPEAT          456..495
FT                   /note="ARM 9"
FT   REGION          519..558
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          572..630
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        541..555
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        594..609
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           2
FT                   /note="N-myristoyl glycine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   630 AA;  67316 MW;  C6841BCF2452CF44 CRC64;
     MGSALSSCCS PRRKNAYEPL LLETEREAVA DLLQYLENRS TTNFFAGSPL AALTTLSFSE
     NVDLQRSAAL AFAEITEKEV REVGRDTLDP VLYLLSSHDP EVQRAASAAL GNLAVNAENK
     LLVVSLGGLE PLIRQMLSPN VEVQCNAVGC ITNLATHDEN KTQIAKSGAL VPLTRLAKSK
     DMRVQRNATG ALLNMTHSDE NRQQLVAAGA IPVLVSLLNS PDTDVQYYCT TALSNIAVDA
     ANRKKLAQSE PKLVQSLVQL MDSQSLKVQC QAALALRNLA SDSKYQLEIV KFGGLKPLLR
     LLHSSYLPLI LSAAACVRNV SIHPANESPI IESGFLQPLI ELLSFDENEE VQCHAISTLR
     NLAASSEKNK GAIVEAGAVE KIKSLVLTVP LAVQSEMTAC VAVLALSDDL KPQLLEMGIC
     EVLIPLTNSP SVEVQGNSAA ALGNLSSKAA EDYAPFNAVW NKPDGGLHAY LVRFLSSADI
     TFQHIAVWTI VQLLEAEDEQ LTNNIRSSPI LISSIRQLAK SPPPSRAGGA PRHDPNDPSA
     GSSEDEFEDG LTDQEGEGEI VSLARRILDL TEVGEEGDEF GERAGRNVPV GSHGQAPGQG
     QTSQVGSMGS EHAALRASVH RALSGGGRDR