VAC8_NEUCR
ID VAC8_NEUCR Reviewed; 559 AA.
AC Q7RXW1;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 4.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Vacuolar protein 8;
GN Name=vac8; ORFNames=B11E5.070, NCU01470;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Functions in both vacuole inheritance and protein targeting
CC from the cytoplasm to vacuole. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Lipid-anchor
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the beta-catenin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAE76142.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; BX842620; CAE76142.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM002240; EAA27511.2; -; Genomic_DNA.
DR RefSeq; XP_956747.2; XM_951654.2.
DR AlphaFoldDB; Q7RXW1; -.
DR SMR; Q7RXW1; -.
DR STRING; 5141.EFNCRP00000007533; -.
DR EnsemblFungi; EAA27511; EAA27511; NCU01470.
DR GeneID; 3872895; -.
DR KEGG; ncr:NCU01470; -.
DR VEuPathDB; FungiDB:NCU01470; -.
DR HOGENOM; CLU_021483_0_0_1; -.
DR InParanoid; Q7RXW1; -.
DR Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:EnsemblFungi.
DR GO; GO:0045121; C:membrane raft; IEA:EnsemblFungi.
DR GO; GO:0071563; C:Myo2p-Vac17p-Vac8p transport complex; IEA:EnsemblFungi.
DR GO; GO:0071561; C:nucleus-vacuole junction; IEA:EnsemblFungi.
DR GO; GO:0000407; C:phagophore assembly site; IEA:EnsemblFungi.
DR GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR GO; GO:0043495; F:protein-membrane adaptor activity; IBA:GO_Central.
DR GO; GO:0000045; P:autophagosome assembly; IEA:EnsemblFungi.
DR GO; GO:0071562; P:nucleus-vacuole junction assembly; IBA:GO_Central.
DR GO; GO:0000425; P:pexophagy; IEA:EnsemblFungi.
DR GO; GO:1903044; P:protein localization to membrane raft; IEA:EnsemblFungi.
DR GO; GO:0034517; P:ribophagy; IEA:EnsemblFungi.
DR GO; GO:0042144; P:vacuole fusion, non-autophagic; IEA:EnsemblFungi.
DR GO; GO:0000011; P:vacuole inheritance; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045156; Vac8.
DR PANTHER; PTHR47249; PTHR47249; 1.
DR Pfam; PF00514; Arm; 6.
DR SMART; SM00185; ARM; 9.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 7.
PE 3: Inferred from homology;
KW Lipoprotein; Membrane; Myristate; Palmitate; Reference proteome; Repeat;
KW Vacuole.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..559
FT /note="Vacuolar protein 8"
FT /id="PRO_0000256215"
FT REPEAT 77..116
FT /note="ARM 1"
FT REPEAT 118..157
FT /note="ARM 2"
FT REPEAT 159..198
FT /note="ARM 3"
FT REPEAT 200..239
FT /note="ARM 4"
FT REPEAT 243..282
FT /note="ARM 5"
FT REPEAT 284..323
FT /note="ARM 6"
FT REPEAT 325..365
FT /note="ARM 7"
FT REPEAT 409..448
FT /note="ARM 8"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
FT LIPID 5
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 559 AA; 60533 MW; 536C6E0E51080789 CRC64;
MGASCSSCCG GKSRDGLYDN VLADSEREAV ADLLQYLENR GETDFFSGEP LRALSTLVYS
ENIDLQRSAS LTFAEITERD VRAVDRDTLE PILFLLQNSD IEVQRAASAA LGNLAVNTDN
KVLIVQLGGL APLIRQMMSP NVEVQCNAVG CITNLATHED NKAKIARSGA LGPLTRLAKS
RDMRVQRNAT GALLNMTHSD ENRQQLVNAG AIPVLVQLLS STDVDVQYYC TTALSNIAVD
ANNRRKLAQT EPRLVQSLVN LMDSSSPKVQ CQAALALRNL ASDEKYQLEI VRASGLGPLL
RLLQSSYLPL ILSAVACIRN ISIHPMNESP IIEAGFLKPL VDLLGSTDNE EIQCHAISTL
RNLAASSDRN KALVLEAGAV QKCKQLVLEV PVTVQSEMTA AIAVLALSDE LKTNLLELGV
FEVLIPLTKS PSIEVQGNSA AALGNLSSKV GDYSVFIHNW NEPSDGIHGY LSRFLASGDA
TFQHIAIWTL LQLLESEDKK LIGLIGKSND IVDMIRQIAN RQIESDNELE DDDEGEVVSL
AQRCLELLGQ GNAKAHIEG
