ID   VAC8_PICPA              Reviewed;         556 AA.
AC   Q5EFZ4;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Vacuolar protein 8;
GN   Name=VAC8;
OS   Komagataella pastoris (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=4922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Thomson M., Dunn W.A. Jr.;
RL   Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Functions in both vacuole inheritance and protein targeting
CC       from the cytoplasm to vacuole. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Lipid-anchor
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the beta-catenin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY886543; AAW78365.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5EFZ4; -.
DR   SMR; Q5EFZ4; -.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043495; F:protein-membrane adaptor activity; IEA:InterPro.
DR   GO; GO:0071562; P:nucleus-vacuole junction assembly; IEA:InterPro.
DR   Gene3D; 1.25.10.10; -; 2.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR000225; Armadillo.
DR   InterPro; IPR045156; Vac8.
DR   PANTHER; PTHR47249; PTHR47249; 1.
DR   Pfam; PF00514; Arm; 7.
DR   SMART; SM00185; ARM; 9.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS50176; ARM_REPEAT; 7.
PE   3: Inferred from homology;
KW   Lipoprotein; Membrane; Myristate; Palmitate; Repeat; Vacuole.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..556
FT                   /note="Vacuolar protein 8"
FT                   /id="PRO_0000256216"
FT   REPEAT          38..74
FT                   /note="ARM 1"
FT   REPEAT          75..115
FT                   /note="ARM 2"
FT   REPEAT          117..156
FT                   /note="ARM 3"
FT   REPEAT          158..197
FT                   /note="ARM 4"
FT   REPEAT          199..238
FT                   /note="ARM 5"
FT   REPEAT          242..281
FT                   /note="ARM 6"
FT   REPEAT          283..322
FT                   /note="ARM 7"
FT   REPEAT          324..364
FT                   /note="ARM 8"
FT   REPEAT          408..447
FT                   /note="ARM 9"
FT   LIPID           2
FT                   /note="N-myristoyl glycine"
FT                   /evidence="ECO:0000250"
FT   LIPID           4
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
FT   LIPID           5
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
FT   LIPID           7
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   556 AA;  60725 MW;  6F51B8FD5390BA58 CRC64;
     MGVCCSCFKR YEHDQSYPPL LAENEREAIS SLLQYLENRS EVDFFTDGPL RALSTLVYSE
     NIDLQRSAAL AFAEVTEKDV RPVTRDVLEP ILILLQSSDA EVQRAACAAL GNLAVNDSNK
     VLIVNMGGLE PLIRQMMSPN IEVQCNAVGC ITNLATQDQN KSKIATSGAL IPLTKLAKSK
     DLRVQRNATG ALLNMTHSLE NRQELVNAGS VPILVQLLSS TDPDVQYYCT TALSNIAVDE
     GNRKKLASTE PKLISQLVQL MDSTSPRVQC QATLALRNLA SDANYQLEIV RAGGLPNLVT
     LLNSTHQPLV LAAVACIRNI SIHPLNEALI IDAGFLKPLV SLLDYNDNVE IQCHAVSTLR
     NLAASSERNR LALLESGAVE KCEKLVLNSP ISVQSEISAC FAILALADDL KMKLLDSNII
     EVLLPLTSSE NGEVCGNAAA ALANLCSRIP DYTIILKNYE QISKFIAKFL NSQNPTFEHI
     ALWTTLQLLE SEDETIKAKL KKQINSGEIS LNNLTTSTLN NHSDTSNIIN NDGEEGEFND
     GDDIINLTQQ ILEMIK