VAC8_SCHPO
ID VAC8_SCHPO Reviewed; 550 AA.
AC O43028;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Vacuolar protein 8;
GN Name=vac8; ORFNames=SPBC354.14c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-548 AND SER-550, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Functions in both vacuole inheritance and protein targeting
CC from the cytoplasm to vacuole. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:16823372}; Lipid-anchor
CC {ECO:0000269|PubMed:16823372}. Vacuole membrane
CC {ECO:0000269|PubMed:16823372}; Lipid-anchor
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the beta-catenin family. {ECO:0000305}.
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DR EMBL; CU329671; CAA17814.2; -; Genomic_DNA.
DR PIR; T40294; T40294.
DR RefSeq; NP_595238.1; NM_001021144.2.
DR AlphaFoldDB; O43028; -.
DR SMR; O43028; -.
DR BioGRID; 277525; 3.
DR STRING; 4896.SPBC354.14c.1; -.
DR iPTMnet; O43028; -.
DR MaxQB; O43028; -.
DR PaxDb; O43028; -.
DR PRIDE; O43028; -.
DR EnsemblFungi; SPBC354.14c.1; SPBC354.14c.1:pep; SPBC354.14c.
DR GeneID; 2541010; -.
DR KEGG; spo:SPBC354.14c; -.
DR PomBase; SPBC354.14c; vac8.
DR VEuPathDB; FungiDB:SPBC354.14c; -.
DR eggNOG; KOG4224; Eukaryota.
DR HOGENOM; CLU_021483_0_0_1; -.
DR InParanoid; O43028; -.
DR OMA; VWDKPDG; -.
DR PhylomeDB; O43028; -.
DR PRO; PR:O43028; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:PomBase.
DR GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043495; F:protein-membrane adaptor activity; ISO:PomBase.
DR GO; GO:0016236; P:macroautophagy; ISO:PomBase.
DR GO; GO:0071562; P:nucleus-vacuole junction assembly; IBA:GO_Central.
DR GO; GO:0042144; P:vacuole fusion, non-autophagic; ISO:PomBase.
DR Gene3D; 1.25.10.10; -; 3.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045156; Vac8.
DR PANTHER; PTHR47249; PTHR47249; 1.
DR Pfam; PF00514; Arm; 9.
DR SMART; SM00185; ARM; 9.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 6.
PE 1: Evidence at protein level;
KW Golgi apparatus; Lipoprotein; Membrane; Myristate; Palmitate;
KW Phosphoprotein; Reference proteome; Repeat; Vacuole.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..550
FT /note="Vacuolar protein 8"
FT /id="PRO_0000256217"
FT REPEAT 75..114
FT /note="ARM 1"
FT REPEAT 116..155
FT /note="ARM 2"
FT REPEAT 157..196
FT /note="ARM 3"
FT REPEAT 198..237
FT /note="ARM 4"
FT REPEAT 241..280
FT /note="ARM 5"
FT REPEAT 282..321
FT /note="ARM 6"
FT REPEAT 323..363
FT /note="ARM 7"
FT REPEAT 454..493
FT /note="ARM 8"
FT MOD_RES 548
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 550
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
FT LIPID 4
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 7
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 550 AA; 60233 MW; 7FFDB55E0A69DB64 CRC64;
MGNCLSCCEK SKDEQYEPLL ADREREAVAD LLSFLEDRNE VNFYSEEPLR ALTILAYSDN
LDLQRSAALA FAEITEKDVR EVDRETIEPV LFLLQSPDAE IQRAASVALG NLAVNAENKA
LVVKLNGLDL LIRQMMSPHV EVQCNAVGCI TNLATLDENK SKIAHSGALG PLTRLAKSKD
IRVQRNATGA LLNMTHSYEN RQQLVSAGTI PVLVSLLPSS DTDVQYYCTT SISNIAVDAV
HRKRLAQSEP KLVRSLIQLM DTSSPKVQCQ AALALRNLAS DERYQIEIVQ SNALPSLLRL
LRSSYLPLIL ASVACIRNIS IHPLNESPII DAGFLRPLVD LLSCTENEEI QCHAVSTLRN
LAASSERNKR AIIEANAIQK LRCLILDAPV SVQSEMTACL AVLALSDEFK SYLLNFGICN
VLIPLTDSMS IEVQGNSAAA LGNLSSNVDD YSRFIECWDS PAGGIHGYLV RFLSSEDSTF
AHIAAWTIVQ LLEAGVPRLT AFIQSSDDII ELLNDIVARD ANNGEYEDGE GDVILLSGRA
LHLIEQDTDS
