VACA1_HELPX
ID VACA1_HELPX Reviewed; 1296 AA.
AC Q48247; Q53434;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Vacuolating cytotoxin autotransporter;
DE Contains:
DE RecName: Full=Vacuolating cytotoxin;
DE Contains:
DE RecName: Full=Vacuolating cytotoxin translocator;
DE Flags: Precursor;
GN Name=vacA;
OS Helicobacter pylori (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=210;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 4867 / CCUG 17874 / NCTC 11638;
RX PubMed=8168917; DOI=10.1128/iai.62.5.1557-1565.1994;
RA Phadnis S.H., Ilver D.J., Janzon L., Normark S., Westblom T.U.;
RT "Pathological significance and molecular characterization of the
RT vacuolating toxin gene of Helicobacter pylori.";
RL Infect. Immun. 62:1557-1565(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX PubMed=8163943; DOI=10.1084/jem.179.5.1653;
RA Telford J.L., Ghiara P., Dell'Orco M., Comanducci M., Burroni D.,
RA Bugnoli M., Tecce M.F., Censini S., Covacci A., Xiang Z., Papini E.,
RA Montecucco C., Parente L., Rappuoli R.;
RT "Gene structure of the Helicobacter pylori cytotoxin and evidence of its
RT key role in gastric disease.";
RL J. Exp. Med. 179:1653-1658(1994).
CC -!- FUNCTION: Induces vacuolation of eukaryotic cells. Causes ulceration
CC and gastric lesions.
CC -!- SUBCELLULAR LOCATION: [Vacuolating cytotoxin autotransporter]:
CC Periplasm {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Vacuolating cytotoxin]: Secreted. Cell surface.
CC -!- SUBCELLULAR LOCATION: [Vacuolating cytotoxin translocator]: Cell outer
CC membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC Note=The cleaved C-terminal fragment (autotransporter domain) is
CC localized in the outer membrane. {ECO:0000250}.
CC -!- DOMAIN: The signal peptide, cleaved at the inner membrane, guides the
CC autotransporter protein to the periplasmic space. Then, insertion of
CC the C-terminal translocator domain in the outer membrane forms a
CC hydrophilic pore for the translocation of the passenger domain to the
CC bacterial cell surface, with subsequent cleavage (By similarity).
CC {ECO:0000250}.
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DR EMBL; U07145; AAA18867.1; -; Unassigned_DNA.
DR EMBL; S72494; AAB30582.1; -; Genomic_DNA.
DR AlphaFoldDB; Q48247; -.
DR SMR; Q48247; -.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 2.40.128.130; -; 1.
DR InterPro; IPR005546; Autotransporte_beta.
DR InterPro; IPR036709; Autotransporte_beta_dom_sf.
DR InterPro; IPR006315; OM_autotransptr_brl.
DR InterPro; IPR003842; Vacuolating_cytotoxin.
DR Pfam; PF02691; VacA; 1.
DR PRINTS; PR01656; VACCYTOTOXIN.
DR SMART; SM00869; Autotransporter; 1.
DR SUPFAM; SSF103515; SSF103515; 1.
DR TIGRFAMs; TIGR01414; autotrans_barl; 1.
DR PROSITE; PS51208; AUTOTRANSPORTER; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Direct protein sequencing; Membrane; Periplasm;
KW Secreted; Signal; Toxin; Transmembrane; Transmembrane beta strand;
KW Virulence.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..1296
FT /note="Vacuolating cytotoxin"
FT /id="PRO_0000387587"
FT CHAIN 34..?
FT /note="Vacuolating cytotoxin autotransporter"
FT /id="PRO_0000002714"
FT CHAIN ?..1296
FT /note="Vacuolating cytotoxin translocator"
FT /evidence="ECO:0000255"
FT /id="PRO_0000002715"
FT DOMAIN 1023..1296
FT /note="Autotransporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00556"
FT REGION 326..380
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 356..380
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1296 AA; 139761 MW; 0D1F3F71AB411447 CRC64;
MEIQQTHRKI NRPLVSLALV GALVSITPQQ SHAAFFTTVI IPAIVGGIAT GTAVGTVSGL
LSWGLKQAEE ANKTPDKPDK VWRIQAGKGF NEFPNKEYDL YRSLLSSKID GGWDWGNAAR
HYWVKGGQQN KLEVDMKDAV GTYTLSGLRN FTGGDLDVNM QKATLRLGQF NGNSFTSYKD
SADRTTRVDF NAKNISIDNF VEINNRVGSG AGRKASSTVL TLQASEGITS DKNAEISLYD
GATLNLASSS VKLMGNVWMG RLQYVGAYLA PSYSTINTSK VTGEVNFNHL TVGDKNAAQA
GIIANKKTNI GTLDLWQSAG LNIIAPPEGG YKDKPNNTPS QSGAKNDKNE SAKNDKQESS
QNNSNTQVIN PPNSAQKTEV QPTQVIDGPF AGGKDTVVNI NRINTNADGT IRVGGFKASL
TTNAAHLHIG KGGVNLSNQA SGRSLIVENL TGNITVDGPL RVNNQVGGYA LAGSSANFEF
KAGTDTKNGT ATFNNDISLG RFVNLKVDAH TANFKGIDTG NGGFNTLDFS GVTDKVNINK
LITASTNVAV KNFNINELIV KTNGISVGEY THFSEDIGSQ SRINTVRLET GTRSLFSGGV
KFKGGEKLVI DEFYYSPWNY FDARNIKNVE ITNKLAFGPQ GSPWGTSKLM FNNLTLGQNA
VMDYSQFSNL TIQGDFINNQ GTINYLVRGG KVATLSVGNA AAMMFNNDID SATGFYKPLI
KINSAQDLIK NTEHVLLKAK IIGYGNVSTG TNGISNVNLE EQFKERLALY NNNNRMDTCV
VRNTDDIKAC GMAIGDQSMV NNPDNYKYLI GKAWKNIGIS KTANGSKISV YYLGNSTPTE
NGGNTTNLPT NTTSNARSAN NALAQNAPFA QPSATPNLVA INQHDFGTIE SVFELANRSK
DIDTLYANSG AQGRDLLQTL LIDSHDAGYA RKMIDATSAN EITKQLNTAT TTLNNIASLE
HKTSGLQTLS LSNAMILNSR LVNLSRRHTN HIDSFAKRLQ ALKDQKFASL ESAAEVLYQF
APKYEKPTNV WANAIGGTSL NNGSNASLYG TSAGVDAYLN GQVEAIVGGF GSYGYSSFNN
RANSLNSGAN NTNFGVYSRI FANQHEFDFE AQGALGSDQS SLNFKSALLQ DLNQSYHYLA
YSAATRASYG YDFAFFRNAL VLKPSVGVSY NHLGSTNFKS NSTNQVALKN GSSSQHLFNA
SANVEARYYY GDTSYFYMNA GVLQEFAHVG SNNAASLNTF KVNAARNPLN THARVMMGGE
LKLAKEVFLN LGVVYLHNLI SNIGHFASNL GMRYSF
