ID   VACA_DICDI              Reviewed;         598 AA.
AC   O15706; Q54HE4;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Vacuolin-A;
GN   Name=vacA; Synonyms=vacA1; ORFNames=DDB_G0289485;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=AX2;
RX   PubMed=9276759; DOI=10.1016/s0960-9822(97)70093-9;
RA   Rauchenberger R., Hacker U., Murphy J., Niewohner J., Maniak M.;
RT   "Coronin and vacuolin identify consecutive stages of a late, actin-coated
RT   endocytic compartment in Dictyostelium.";
RL   Curr. Biol. 7:215-218(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=9394012; DOI=10.1242/jcs.111.1.61;
RA   Jenne N., Rauchenberger R., Hacker U., Kast T., Maniak M.;
RT   "Targeted gene disruption reveals a role for vacuolin B in the late
RT   endocytic pathway and exocytosis.";
RL   J. Cell Sci. 111:61-70(1998).
RN   [4]
RP   DOMAIN, AND SUBCELLULAR LOCATION.
RX   PubMed=16750281; DOI=10.1016/j.ejcb.2006.04.010;
RA   Wienke D., Drengk A., Schmauch C., Jenne N., Maniak M.;
RT   "Vacuolin, a flotillin/reggie-related protein from Dictyostelium
RT   oligomerizes for endosome association.";
RL   Eur. J. Cell Biol. 85:991-1000(2006).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=AX2;
RX   PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA   Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA   Soldati T.;
RT   "Proteomics fingerprinting of phagosome maturation and evidence for the
RT   role of a Galpha during uptake.";
RL   Mol. Cell. Proteomics 5:2228-2243(2006).
CC   -!- SUBCELLULAR LOCATION: Endosome membrane; Peripheral membrane protein.
CC       Lysosome. Note=Post-lysosome.
CC   -!- DEVELOPMENTAL STAGE: Expressed during all developmental stages.
CC   -!- SIMILARITY: Belongs to the vacuolin family. {ECO:0000305}.
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DR   EMBL; AF014049; AAC47709.1; -; mRNA.
DR   EMBL; AAFI02000141; EAL62674.1; -; Genomic_DNA.
DR   RefSeq; XP_636194.1; XM_631102.1.
DR   AlphaFoldDB; O15706; -.
DR   STRING; 44689.DDB0191505; -.
DR   PaxDb; O15706; -.
DR   ABCD; O15706; 5 sequenced antibodies.
DR   EnsemblProtists; EAL62674; EAL62674; DDB_G0289485.
DR   GeneID; 8627181; -.
DR   KEGG; ddi:DDB_G0289485; -.
DR   dictyBase; DDB_G0289485; vacA.
DR   eggNOG; ENOG502S9G3; Eukaryota.
DR   HOGENOM; CLU_461128_0_0_1; -.
DR   InParanoid; O15706; -.
DR   OMA; GKNSAFT; -.
DR   PhylomeDB; O15706; -.
DR   PRO; PR:O15706; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0032009; C:early phagosome; IDA:dictyBase.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005770; C:late endosome; IDA:dictyBase.
DR   GO; GO:0016020; C:membrane; IDA:dictyBase.
DR   GO; GO:0140220; C:pathogen-containing vacuole; IDA:dictyBase.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0032010; C:phagolysosome; IDA:dictyBase.
DR   GO; GO:0005773; C:vacuole; IDA:dictyBase.
DR   GO; GO:0061951; P:establishment of protein localization to plasma membrane; IGI:dictyBase.
DR   GO; GO:0006910; P:phagocytosis, recognition; IGI:dictyBase.
DR   GO; GO:0090382; P:phagosome maturation; IGI:dictyBase.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IGI:dictyBase.
DR   GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR   Gene3D; 3.30.479.30; -; 1.
DR   InterPro; IPR001107; Band_7.
DR   InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR   Pfam; PF01145; Band_7; 1.
DR   SUPFAM; SSF117892; SSF117892; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Endosome; Lysosome; Membrane; Reference proteome.
FT   CHAIN           1..598
FT                   /note="Vacuolin-A"
FT                   /id="PRO_0000328355"
FT   COILED          482..539
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   598 AA;  66288 MW;  883D2DED8B1C4429 CRC64;
     MIEGSGSKTP KRNSDEISVK SLSIHEANTL SSEHEHESGN EELTLSQIVI RSDFANESSE
     RQSKIILDRF NQGIGKNSAF TDSPALGRGL SRVDVSYFVM GHRNQVAELY DPRGQRFSTV
     LTPDPTVNIK YVGPVSKTAH YFGAYGKHVL NVPAGHYAKA FSKNRPVLYG EGPHVIIDPT
     FQFDENNGFV NQQEPFIEHS TINILRIPAG KIAKVWIGTQ PHILESRRDP YVFVDAQFKL
     VSPDGAKKAQ LFESSSCTFV EHGSIKRIIP HTGEVAITYN NGILTIIPTP KDGKPVIIDS
     PTHNFEGFIS TSLQTCLFPS KETKQQALAD NKSALADEIN LKIFQTRDSL RVGVVLVVAF
     KIVDPELAIT KLGKEGIINH IENVSFADMG KAIQLSTLQE IMYFNSIKPG QATNDDSVQT
     IQDRVKSHLA RDLFDYGVEL SRLQIETMKV LDTEIAKKLA GQSVTSAEFT TKQATLVKEY
     DIKTTEARLK AETDNIALEQ RNKAIISESQ AKLSSAQREA ESLLITAEAQ KKASELQGEL
     YTKYPILAEI ELARIKAEAL KNATLYITPQ DAGAFMNSPL VYFDKMMNAN NTIQQKKN