VACA_HELPY
ID VACA_HELPY Reviewed; 1290 AA.
AC P55981;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Vacuolating cytotoxin autotransporter;
DE Contains:
DE RecName: Full=Vacuolating cytotoxin;
DE Contains:
DE RecName: Full=Vacuolating cytotoxin translocator;
DE Flags: Precursor;
GN Name=vacA; OrderedLocusNames=HP_0887;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
CC -!- FUNCTION: Induces vacuolation of eukaryotic cells. Causes ulceration
CC and gastric lesions.
CC -!- SUBCELLULAR LOCATION: [Vacuolating cytotoxin autotransporter]:
CC Periplasm {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: [Vacuolating cytotoxin]: Secreted. Cell surface.
CC -!- SUBCELLULAR LOCATION: [Vacuolating cytotoxin translocator]: Cell outer
CC membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC Note=The cleaved C-terminal fragment (autotransporter domain) is
CC localized in the outer membrane. {ECO:0000250}.
CC -!- DOMAIN: The signal peptide, cleaved at the inner membrane, guides the
CC autotransporter protein to the periplasmic space. Then, insertion of
CC the C-terminal translocator domain in the outer membrane forms a
CC hydrophilic pore for the translocation of the passenger domain to the
CC bacterial cell surface, with subsequent cleavage (By similarity).
CC {ECO:0000250}.
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DR EMBL; AE000511; AAD07935.1; -; Genomic_DNA.
DR PIR; G64630; G64630.
DR RefSeq; NP_207680.1; NC_000915.1.
DR RefSeq; WP_000405496.1; NC_000915.1.
DR AlphaFoldDB; P55981; -.
DR SMR; P55981; -.
DR DIP; DIP-3244N; -.
DR IntAct; P55981; 15.
DR MINT; P55981; -.
DR TCDB; 1.C.9.1.1; the vacuolating cytotoxin (vaca) family.
DR PaxDb; P55981; -.
DR ABCD; P55981; 6 sequenced antibodies.
DR EnsemblBacteria; AAD07935; AAD07935; HP_0887.
DR KEGG; hpy:HP_0887; -.
DR PATRIC; fig|85962.8.peg.919; -.
DR OMA; NQGTINY; -.
DR PhylomeDB; P55981; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 2.40.128.130; -; 1.
DR InterPro; IPR005546; Autotransporte_beta.
DR InterPro; IPR036709; Autotransporte_beta_dom_sf.
DR InterPro; IPR003842; Vacuolating_cytotoxin.
DR Pfam; PF03797; Autotransporter; 1.
DR Pfam; PF02691; VacA; 1.
DR PRINTS; PR01656; VACCYTOTOXIN.
DR SMART; SM00869; Autotransporter; 1.
DR SUPFAM; SSF103515; SSF103515; 1.
DR PROSITE; PS51208; AUTOTRANSPORTER; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Membrane; Periplasm; Reference proteome; Secreted;
KW Signal; Toxin; Transmembrane; Transmembrane beta strand; Virulence.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..1290
FT /note="Vacuolating cytotoxin autotransporter"
FT /id="PRO_0000387586"
FT CHAIN 34..?
FT /note="Vacuolating cytotoxin"
FT /id="PRO_0000022651"
FT CHAIN ?..1290
FT /note="Vacuolating cytotoxin translocator"
FT /evidence="ECO:0000255"
FT /id="PRO_0000022652"
FT DOMAIN 1018..1290
FT /note="Autotransporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00556"
FT REGION 326..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1290 AA; 139312 MW; F48B23513447A1AC CRC64;
MEIQQTHRKI NRPLVSLALV GALVSITPQQ SHAAFFTTVI IPAIVGGIAT GAAVGTVSGL
LGWGLKQAEE ANKTPDKPDK VWRIQAGKGF NEFPNKEYDL YRSLLSSKID GGWDWGNAAT
HYWVKGGQWN KLEVDMKDAV GTYNLSGLRN FTGGDLDVNM QKATLRLGQF NGNSFTSYKD
SADRTTRVDF NAKNILIDNF LEINNRVGSG AGRKASSTVL TLQASEGITS SKNAEISLYD
GATLNLASNS VKLMGNVWMG RLQYVGAYLA PSYSTINTSK VTGEVNFNHL TVGDHNAAQA
GIIASNKTHI GTLDLWQSAG LNIIAPPEGG YKDKPKDKPS NTTQNNANNN QQNSAQNNSN
TQVINPPNSA QKTEIQPTQV IDGPFAGGKD TVVNIDRINT NADGTIKVGG YKASLTTNAA
HLHIGKGGIN LSNQASGRTL LVENLTGNIT VDGPLRVNNQ VGGYALAGSS ANFEFKAGTD
TKNGTATFNN DISLGRFVNL KVDAHTANFK GIDTGNGGFN TLDFSGVTGK VNINKLITAS
TNVAVKNFNI NELVVKTNGV SVGEYTHFSE DIGSQSRINT VRLETGTRSI FSGGVKFKSG
EKLVIDEFYY SPWNYFDARN IKNVEITRKF ASSTPENPWG TSKLMFNNLT LGQNAVMDYS
QFSNLTIQGD FINNQGTINY LVRGGQVATL NVGNAAAMFF SNNVDSATGF YQPLMKINSA
QDLIKNKEHV LLKAKIIGYG NVSLGTNSIS NVNLIEQFKE RLALYNNNNR MDICVVRNTD
DIKACGTAIG NQSMVNNPDN YKYLIGKAWK NIGISKTANG SKISVYYLGN STPTEKGGNT
TNLPTNTTSN VRSANNALAQ NAPFAQPSAT PNLVAINQHD FGTIESVFEL ANRSKDIDTL
YANSGAQGRD LLQTLLIDSH DAGYARQMID NTSTGEITKQ LNAATTTLNN IASLEHKTSS
LQTLSLSNAM ILNSRLVNLS RRHTNNIDSF AQRLQALKDQ KFASLESAAE VLYQFAPKYE
KPTNVWANAI GGTSLNNGGN ASLYGTSAGV DAYLNGEVEA IVGGFGSYGY SSFNNQANSL
NSGANNTNFG VYSRIFANQH EFDFEAQGAL GSDQSSLNFK SALLRDLNQS YNYLAYSAAT
RASYGYDFAF FRNALVLKPS VGVSYNHLGS TNFKSNSNQV ALKNGSSSQH LFNASANVEA
RYYYGDTSYF YMNAGVLQEF ANFGSSNAVS LNTFKVNAAH NPLSTHARVM MGGELKLAKE
VFLNLGFVYL HNLISNIGHF ASNLGMRYSF
