VACB_DICDI
ID VACB_DICDI Reviewed; 592 AA.
AC Q54WZ2; O15707;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Vacuolin-B;
GN Name=vacB; ORFNames=DDB_G0279191;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=AX2;
RX PubMed=9394012; DOI=10.1242/jcs.111.1.61;
RA Jenne N., Rauchenberger R., Hacker U., Kast T., Maniak M.;
RT "Targeted gene disruption reveals a role for vacuolin B in the late
RT endocytic pathway and exocytosis.";
RL J. Cell Sci. 111:61-70(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION, DOMAIN, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=16750281; DOI=10.1016/j.ejcb.2006.04.010;
RA Wienke D., Drengk A., Schmauch C., Jenne N., Maniak M.;
RT "Vacuolin, a flotillin/reggie-related protein from Dictyostelium
RT oligomerizes for endosome association.";
RL Eur. J. Cell Biol. 85:991-1000(2006).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- FUNCTION: Negative regulator of late steps of the endocytic pathway.
CC {ECO:0000269|PubMed:16750281, ECO:0000269|PubMed:9394012}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000305|PubMed:16750281}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane; Peripheral membrane protein.
CC Lysosome membrane; Peripheral membrane protein. Note=Post-lysosome.
CC -!- DEVELOPMENTAL STAGE: Expression restricted to vegetative phase.
CC -!- DOMAIN: The coiled-coil region is essential for oligomerization and
CC binding to vacuolar structures. {ECO:0000269|PubMed:16750281}.
CC -!- SIMILARITY: Belongs to the vacuolin family. {ECO:0000305}.
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DR EMBL; AF014050; AAB66554.1; -; mRNA.
DR EMBL; AAFI02000030; EAL67744.1; -; Genomic_DNA.
DR RefSeq; XP_641785.1; XM_636693.1.
DR AlphaFoldDB; Q54WZ2; -.
DR STRING; 44689.DDB0214913; -.
DR PaxDb; Q54WZ2; -.
DR PRIDE; Q54WZ2; -.
DR ABCD; Q54WZ2; 4 sequenced antibodies.
DR EnsemblProtists; EAL67744; EAL67744; DDB_G0279191.
DR GeneID; 8621982; -.
DR KEGG; ddi:DDB_G0279191; -.
DR dictyBase; DDB_G0279191; vacB.
DR eggNOG; ENOG502S9G3; Eukaryota.
DR HOGENOM; CLU_461128_0_0_1; -.
DR InParanoid; Q54WZ2; -.
DR OMA; HIDINDK; -.
DR PhylomeDB; Q54WZ2; -.
DR PRO; PR:Q54WZ2; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0032009; C:early phagosome; IDA:dictyBase.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IDA:dictyBase.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0005773; C:vacuole; IDA:dictyBase.
DR GO; GO:0061951; P:establishment of protein localization to plasma membrane; IGI:dictyBase.
DR GO; GO:0006887; P:exocytosis; IMP:dictyBase.
DR GO; GO:0006910; P:phagocytosis, recognition; IGI:dictyBase.
DR GO; GO:0090382; P:phagosome maturation; IGI:dictyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IGI:dictyBase.
DR GO; GO:0009617; P:response to bacterium; IMP:dictyBase.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR Pfam; PF01145; Band_7; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endosome; Lysosome; Membrane; Reference proteome.
FT CHAIN 1..592
FT /note="Vacuolin-B"
FT /id="PRO_0000328356"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 491..555
FT /note="Oligomerization domain"
FT COILED 480..534
FT /evidence="ECO:0000255"
FT CONFLICT 388
FT /note="K -> R (in Ref. 1; AAB66554)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 592 AA; 66211 MW; 506B56E3557D294E CRC64;
MIESSSFMKK TSSENSIGSR SNIHEASTFS SEHENGDQSL TISQIVIRSD FANETSERQS
KIILERFNEG IKNTAFTDSP VLGRAQPRLD VSYFAMAHRN QIVELFDPRG QRFGTVYSAD
PTVEIRYLGS VAKTCRYFGS FGKHVLNVPS GHFAKAMTKN RPIIYGEGPH VIIDPTFEFD
ERYGFVNQQE PFINHSTINI LRVPSGKVAK VWIGTQPLIL ESRREPYVFV DAQFKMVEDT
SESGNNKYFK NSSATFMEHG SIKRIIPHTG EVAITYNNGI LTIIPPPKDG KPVIIDSPTH
NFEGFIQTSL QTCLFPSKET KQQAIKDNKN ATSDEVNLKI FQTRDSLRVG VVLVVAFRIV
DPEIALTKLG KEGIINHIEN VSFADMGKAI QLSTLQEIMY FNDTKPSANS TNETVHTIQD
RVKSHLARDL CEYGIELARL QIETMKVLDS EIAKKLAGQS VTSAEFTTKQ ATLVKEYDIK
TTEARLKAET DNIALEQKGK AIIAEAQAKL ESAQKQAQAL LITAEAQKKV QEMQGELFTK
YPILAEIELA KIKSEALKSA TLYITPQDAG NFMNSPLVYM DRLLGHQQKL EK
