VACHA_DANRE
ID VACHA_DANRE Reviewed; 513 AA.
AC Q08C75;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Probable vesicular acetylcholine transporter-A;
DE Short=VAChT-A {ECO:0000250|UniProtKB:Q16572};
DE AltName: Full=Solute carrier family 18 member 3-A {ECO:0000312|ZFIN:ZDB-GENE-060929-990};
GN Name=slc18a3a {ECO:0000312|ZFIN:ZDB-GENE-060929-990};
GN ORFNames=si:ch211-232k3.4, zgc:153442;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000312|EMBL:CAX14609.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye {ECO:0000312|EMBL:AAI24355.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in acetylcholine transport into synaptic vesicles.
CC {ECO:0000250|UniProtKB:Q16572}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Vesicular
CC transporter family. {ECO:0000255}.
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DR EMBL; BX855592; CAX14609.1; -; Genomic_DNA.
DR EMBL; BC124354; AAI24355.1; -; mRNA.
DR RefSeq; NP_001071018.1; NM_001077550.1.
DR AlphaFoldDB; Q08C75; -.
DR SMR; Q08C75; -.
DR STRING; 7955.ENSDARP00000012428; -.
DR PaxDb; Q08C75; -.
DR PRIDE; Q08C75; -.
DR Ensembl; ENSDART00000026765; ENSDARP00000012428; ENSDARG00000006356.
DR GeneID; 559347; -.
DR KEGG; dre:559347; -.
DR CTD; 559347; -.
DR ZFIN; ZDB-GENE-060929-990; slc18a3a.
DR eggNOG; KOG3764; Eukaryota.
DR GeneTree; ENSGT00940000159449; -.
DR HOGENOM; CLU_001265_10_9_1; -.
DR InParanoid; Q08C75; -.
DR OMA; ACKNFEQ; -.
DR OrthoDB; 956763at2759; -.
DR PhylomeDB; Q08C75; -.
DR TreeFam; TF313494; -.
DR Reactome; R-DRE-264642; Acetylcholine Neurotransmitter Release Cycle.
DR Reactome; R-DRE-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-DRE-8856828; Clathrin-mediated endocytosis.
DR PRO; PR:Q08C75; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 13.
DR Bgee; ENSDARG00000006356; Expressed in larva and 6 other tissues.
DR ExpressionAtlas; Q08C75; baseline.
DR GO; GO:0030121; C:AP-1 adaptor complex; IBA:GO_Central.
DR GO; GO:0030122; C:AP-2 adaptor complex; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043195; C:terminal bouton; IBA:GO_Central.
DR GO; GO:0005277; F:acetylcholine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004734; Multidrug-R.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00880; 2_A_01_02; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Neurotransmitter transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..513
FT /note="Probable vesicular acetylcholine transporter-A"
FT /id="PRO_0000401127"
FT TOPO_DOM 1..39
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..98
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..156
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..215
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..257
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..296
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 318..327
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 328..348
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 349..353
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 375..390
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 412..418
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 440..513
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 475..513
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 485..503
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 82
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 86
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 292
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 513 AA; 55760 MW; 9FBBAA62F18BF6B1 CRC64;
MATEESGGLA QTAAVKLSEM GERTKQLGNA IQDPERQRRI ILVIVCVALL LDNMLYMVIV
PIVPDYLAHL ESESEQAHVK GNSSINITQN ENFDLQIGVL FASKAILQLL VNPLTGTFID
RVGYDIPLLI GLSIMFVSTC IFAFAENYAT LFMARSLQGL GSAFADTSGI AMIADKYAEE
SERSRALGIA LAFISFGSLA APPFGGVLYE FAGKRFPFIA LACVCLADGI LCLTVLKPFS
SRTRENMPVG TPIYKLMIDP YIAVVAGALT TCNIPLAFLE PTIANWMEET MNASQWQIGI
TWLPAFFPHI LGVYLTVKLA AKYPHLQWFY GALGMVIIGA SSCIVPACKN FEQLIIPLCG
VCFGIALVDT ALLPTLAFLV DVRHVSVYGS VYAIADISYC VAYALGPIVA GKIVHDLGFV
QLNLGMGLAN VLYAPALLLL RNVSLMKPSH SERNMLLEEG ATGLYDTIRM EERQRKKHGY
SSSGNCVPID ENGTFAGQSK SFSEEETSEP EYI
