VACHB_DANRE
ID VACHB_DANRE Reviewed; 493 AA.
AC P59845;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2003, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Probable vesicular acetylcholine transporter-B;
DE Short=VAChT-B;
DE AltName: Full=Solute carrier family 18 member 3-B;
GN Name=slc18a3b; Synonyms=slc18a3, vacht; ORFNames=zgc:64220;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in acetylcholine transport into synaptic vesicles.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Vesicular
CC transporter family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC054646; AAH54646.1; -; mRNA.
DR RefSeq; NP_957401.1; NM_201107.1.
DR AlphaFoldDB; P59845; -.
DR SMR; P59845; -.
DR GeneID; 394082; -.
DR KEGG; dre:394082; -.
DR CTD; 394082; -.
DR ZFIN; ZDB-GENE-040426-1410; slc18a3b.
DR InParanoid; P59845; -.
DR OrthoDB; 956763at2759; -.
DR PhylomeDB; P59845; -.
DR PRO; PR:P59845; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0030121; C:AP-1 adaptor complex; IBA:GO_Central.
DR GO; GO:0030122; C:AP-2 adaptor complex; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043195; C:terminal bouton; IBA:GO_Central.
DR GO; GO:0005277; F:acetylcholine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004734; Multidrug-R.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00880; 2_A_01_02; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Neurotransmitter transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..493
FT /note="Probable vesicular acetylcholine transporter-B"
FT /id="PRO_0000127521"
FT TOPO_DOM 1..39
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..86
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..113
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..143
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..203
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..247
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..284
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..315
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 337..347
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 369..378
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 400..406
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 428..493
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 467..493
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 493 AA; 53052 MW; D3A450870750B842 CRC64;
MQSTGAPGLA QSAVLQLSAM GERSRELGGA LREPERKRRL LLVVVCVALL LDNMLYMVIV
PIIPDYLADL RGERGNSSAD LDIQIGVLFA SKALLQLLVN PLSGTFIDRV GYDLPLLIGL
LVMFLSTCIF AFAENYGTLF AARSLQGLGS AFADTSGIAM IADKFTEEAE RSRALGIALA
FISFGSLVAP PFGGILYEFA GKRVPFIVLA CVCLADGVLL LTVVKPFSDR TRENMPVGTP
IHRLMVDPYI AVVAGALTVC NIPLAFLEPT IANWMESTMD ASKWQMGLVW LPAFLPHVLG
VYITVRLAAR YPERQWFYGA LGMVIIGASS CTVPACKTFG ELVFPLCGIC FGIALVDTAL
LPTLAFLVDV RHVSVYGSVY AIADISYSVA YAMGPVVAGQ IVHNLGFVQL NLGMGLVNVL
YAPALLLLRP VCQIKPSFSE RNVLLEEGPS GLYDSIRLEE RALRRKGLSA GAGTEHGLRG
RSEEEEESGP ESA
