VACHT_DROME
ID VACHT_DROME Reviewed; 578 AA.
AC O17444; Q9VE42;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2003, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Vesicular acetylcholine transporter;
DE Short=VAChT;
GN Name=VAChT; ORFNames=CG32848;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Head;
RX PubMed=9446576; DOI=10.1074/jbc.273.5.2706;
RA Kitamoto T., Wang W., Salvaterra P.M.;
RT "Structure and organization of the Drosophila cholinergic locus.";
RL J. Biol. Chem. 273:2706-2713(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Involved in acetylcholine transport into synaptic vesicles.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Vesicular
CC transporter family. {ECO:0000305}.
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DR EMBL; AF030197; AAB86609.1; -; mRNA.
DR EMBL; AE014297; AAF55587.2; -; Genomic_DNA.
DR RefSeq; NP_477138.1; NM_057790.5.
DR AlphaFoldDB; O17444; -.
DR SMR; O17444; -.
DR IntAct; O17444; 2.
DR STRING; 7227.FBpp0083086; -.
DR GlyGen; O17444; 2 sites.
DR PaxDb; O17444; -.
DR DNASU; 42795; -.
DR EnsemblMetazoa; FBtr0083671; FBpp0083086; FBgn0270928.
DR GeneID; 42795; -.
DR KEGG; dme:Dmel_CG32848; -.
DR UCSC; CG32848-RA; d. melanogaster.
DR CTD; 42795; -.
DR FlyBase; FBgn0270928; VAChT.
DR VEuPathDB; VectorBase:FBgn0270928; -.
DR eggNOG; KOG3764; Eukaryota.
DR GeneTree; ENSGT00940000159449; -.
DR HOGENOM; CLU_001265_10_9_1; -.
DR InParanoid; O17444; -.
DR OMA; PDYIAHM; -.
DR OrthoDB; 956763at2759; -.
DR PhylomeDB; O17444; -.
DR Reactome; R-DME-264642; Acetylcholine Neurotransmitter Release Cycle.
DR Reactome; R-DME-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-DME-8856828; Clathrin-mediated endocytosis.
DR BioGRID-ORCS; 42795; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42795; -.
DR PRO; PR:O17444; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0270928; Expressed in brain and 8 other tissues.
DR ExpressionAtlas; O17444; baseline and differential.
DR Genevisible; O17444; DM.
DR GO; GO:0030121; C:AP-1 adaptor complex; IBA:GO_Central.
DR GO; GO:0030122; C:AP-2 adaptor complex; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043195; C:terminal bouton; IDA:FlyBase.
DR GO; GO:0005277; F:acetylcholine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004734; Multidrug-R.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00880; 2_A_01_02; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Neurotransmitter transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..578
FT /note="Vesicular acetylcholine transporter"
FT /id="PRO_0000127526"
FT TOPO_DOM 1..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..98
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..154
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..187
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..215
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..263
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..299
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..330
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 331..351
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 352..355
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..387
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 409..413
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 435..578
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 507..578
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 507..567
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 78
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 293
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 97
FT /note="T -> S (in Ref. 1; AAB86609)"
FT /evidence="ECO:0000305"
FT CONFLICT 160
FT /note="A -> V (in Ref. 1; AAB86609)"
FT /evidence="ECO:0000305"
FT CONFLICT 272
FT /note="L -> M (in Ref. 1; AAB86609)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 578 AA; 64314 MW; 4E73402CB934E4B5 CRC64;
MASFQIPVIN LEVREVKDIV WEKIQEPVNQ RRLILVIVSI ALLLDNMLYM VIVPIIPDYL
REIGSFDDGP TPPPLRDNIT GKIIPVHHDH HGQDSATGIL FASKAIVQLM VNPFSGGLID
KIGYDLPMMI GLTIMFFSTA VFACGSSYSV LFFARSLQGA GSAFADTAGL AMIADRFTEE
NERSQALGIA LAFISFGCLV APPFGGALYQ FAGKEVPFLI LALVCLLDGL MLLLVMKPVK
EAMKQSKDVQ DQVIPIWRLL MDPYIAVCAG ALTMSNVALA FLEPTISLWM EDNMTTDNWK
IGMVWLPAFF PHVLGVVITV KMARKYPQHQ WLMAAGGLAL EGFSCFIIPF CSGYKMLMLP
ICVICFGIAL IDTALLPTLG YLVDVRYVSV YGSIYAIADI SYSIAYAVGP IIAGGVVEAI
GFTALNFLIA FSNLAYVPVL RKLRNIYDFK PFENEANILM QDPPNKEYQT YVMHDQKPVE
GGVKNHLEYG QQYQQEQETN LDDQQYEYQQ QQQGYQQGYQ QDQGYQPGYQ EQGGSYAPQG
QPRVANPFQQ QQQQQQQQQQ QVQSRGPAAP ANPFRQGF
