VACHT_RAT
ID VACHT_RAT Reviewed; 530 AA.
AC Q62666; Q64351;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2003, sequence version 2.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=Vesicular acetylcholine transporter;
DE Short=VAChT;
DE Short=rVAT;
DE AltName: Full=Solute carrier family 18 member 3;
GN Name=Slc18a3; Synonyms=Vacht, Vat;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Brain stem;
RX PubMed=7938002; DOI=10.1073/pnas.91.22.10620;
RA Roghani A., Feldman J., Kohan S.A., Shirzadi A., Gundersen C.B., Brecha N.,
RA Edwards R.H.;
RT "Molecular cloning of a putative vesicular transporter for acetylcholine.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:10620-10624(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Pheochromocytoma;
RX PubMed=8071310; DOI=10.1016/s0021-9258(17)31734-9;
RA Erickson J.D., Varoqui H., Schafer M.K.-H., Modi W., Diebler M.-F.,
RA Weihe E., Rand J.B., Eiden L.E., Bonner T.I., Usdin T.B.;
RT "Functional identification of a vesicular acetylcholine transporter and its
RT expression from a 'cholinergic' gene locus.";
RL J. Biol. Chem. 269:21929-21932(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Spinal cord;
RX PubMed=8071313; DOI=10.1016/s0021-9258(17)31738-6;
RA Bejanin S., Cervini R., Mallet J., Berrard S.;
RT "A unique gene organization for two cholinergic markers, choline
RT acetyltransferase and a putative vesicular transporter of acetylcholine.";
RL J. Biol. Chem. 269:21944-21947(1994).
CC -!- FUNCTION: Involved in acetylcholine transport into synaptic vesicles.
CC {ECO:0000269|PubMed:8071313}.
CC -!- SUBUNIT: Interacts with SEC14L1. {ECO:0000250|UniProtKB:Q16572}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: High expression in all major cholinergic cell
CC groups, including peripheral postganglionic parasympathetic cells,
CC preganglionic sympathetic and parasympathetic cells, ventral spinal
CC cord and brainstem motoneurons, cell groups in the basal forebrain, the
CC habenula and the corpus striatum. Weakly expressed in the cortex and
CC hippocampus. {ECO:0000269|PubMed:7938002, ECO:0000269|PubMed:8071310,
CC ECO:0000269|PubMed:8071313}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Vesicular
CC transporter family. {ECO:0000305}.
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DR EMBL; U09838; AAA50831.1; -; mRNA.
DR EMBL; U09211; AAA20498.1; -; mRNA.
DR EMBL; X80395; CAA56604.1; -; Genomic_DNA.
DR RefSeq; NP_113851.1; NM_031663.2.
DR AlphaFoldDB; Q62666; -.
DR SMR; Q62666; -.
DR BindingDB; Q62666; -.
DR ChEMBL; CHEMBL2125; -.
DR DrugCentral; Q62666; -.
DR GlyGen; Q62666; 2 sites.
DR iPTMnet; Q62666; -.
DR PhosphoSitePlus; Q62666; -.
DR ABCD; Q62666; 2 sequenced antibodies.
DR GeneID; 60422; -.
DR KEGG; rno:60422; -.
DR UCSC; RGD:62072; rat.
DR CTD; 6572; -.
DR RGD; 62072; Slc18a3.
DR InParanoid; Q62666; -.
DR OrthoDB; 956763at2759; -.
DR PhylomeDB; Q62666; -.
DR Reactome; R-RNO-264642; Acetylcholine Neurotransmitter Release Cycle.
DR Reactome; R-RNO-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-RNO-8856828; Clathrin-mediated endocytosis.
DR PRO; PR:Q62666; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0030121; C:AP-1 adaptor complex; IDA:RGD.
DR GO; GO:0030122; C:AP-2 adaptor complex; IDA:RGD.
DR GO; GO:0030424; C:axon; IDA:RGD.
DR GO; GO:0043679; C:axon terminus; IDA:RGD.
DR GO; GO:0098981; C:cholinergic synapse; IDA:SynGO.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:RGD.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; IDA:SynGO.
DR GO; GO:0031594; C:neuromuscular junction; IDA:SynGO.
DR GO; GO:0043005; C:neuron projection; ISO:RGD.
DR GO; GO:0008021; C:synaptic vesicle; IDA:RGD.
DR GO; GO:0043195; C:terminal bouton; ISO:RGD.
DR GO; GO:0005277; F:acetylcholine transmembrane transporter activity; IDA:RGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015870; P:acetylcholine transport; IMP:RGD.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0098700; P:neurotransmitter loading into synaptic vesicle; IDA:SynGO.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Neurotransmitter transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..530
FT /note="Vesicular acetylcholine transporter"
FT /id="PRO_0000127520"
FT TOPO_DOM 1..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..125
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..152
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 174..182
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 204..213
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235..242
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..289
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 311..325
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..356
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 378..388
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 410..422
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..447
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 469..530
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 471..530
FT /note="Mediates interaction with SEC14L1"
FT /evidence="ECO:0000250|UniProtKB:O35304"
FT REGION 504..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 92
FT /note="A -> T (in Ref. 1; AAA50831)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 530 AA; 56537 MW; 07720DA749527438 CRC64;
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY MVIVPIVPDY
IAHMRGGSEG PTLVSEVWEP TLPPPTLANA SAYLANTSAS PTAAGSARSI LRPRYPTESE
DVKIGVLFAS KAILQLLVNP LSGPFIDRMS YDVPLLIGLG VMFASTVMFA FAEDYATLFA
ARSLQGLGSA FADTSGIAMI ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG
KRVPFLVLAA VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA RYPHLQWLYG
ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA LLPTLAFLVD VRHVSVYGSV
YAIADISYSV AYALGPIVAG HIVHSLGFEQ LSLGMGLANL LYAPVLLLLR NVGLLTRSRS
ERDVLLDEPP QGLYDAVRLR EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS
