VAD1_ARATH
ID VAD1_ARATH Reviewed; 598 AA.
AC F4HVW5;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Protein VASCULAR ASSOCIATED DEATH 1, chloroplastic {ECO:0000303|PubMed:15269331};
DE Flags: Precursor;
GN Name=VAD1 {ECO:0000303|PubMed:15269331};
GN OrderedLocusNames=At1g02120 {ECO:0000312|Araport:AT1G02120};
GN ORFNames=T7I23.28 {ECO:0000312|EMBL:U89959};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY PSEUDOMONAS SYRINGAE.
RC STRAIN=cv. Wassilewskija-4;
RX PubMed=15269331; DOI=10.1105/tpc.104.022038;
RA Lorrain S., Lin B., Auriac M.C., Kroj T., Saindrenan P., Nicole M.,
RA Balague C., Roby D.;
RT "Vascular associated death1, a novel GRAM domain-containing protein, is a
RT regulator of cell death and defense responses in vascular tissues.";
RL Plant Cell 16:2217-2232(2004).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Wassilewskija-4;
RX PubMed=17720753; DOI=10.1104/pp.107.106302;
RA Bouchez O., Huard C., Lorrain S., Roby D., Balague C.;
RT "Ethylene is one of the key elements for cell death and defense response
RT control in the Arabidopsis lesion mimic mutant vad1.";
RL Plant Physiol. 145:465-477(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Involved in ethylene- and salicylic acid-dependent cell death
CC control associated with cells in the vicinity of vascular bundles.
CC {ECO:0000269|PubMed:15269331, ECO:0000269|PubMed:17720753}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}. Plastid, chloroplast {ECO:0000255}.
CC -!- INDUCTION: Expressed in response to pathogen infection (e.g.
CC Pseudomonas syringae) at the vicinity of the hypersensitive lesions.
CC {ECO:0000269|PubMed:15269331}.
CC -!- DISRUPTION PHENOTYPE: Lesion mimic mutant with a light conditional
CC appearance of propagative hypersensitive response (HR)-like lesions
CC along the vascular system, and associated with expression of defense
CC genes, production of high levels of salicylic acid (SA)
CC (PubMed:15269331). Increased resistance to virulent and avirulent
CC strains of Pseudomonas syringae pv tomato (PubMed:15269331,
CC PubMed:17720753). This necrosis symptoms are ethylene-dependent and
CC associated with ethylene accumulation (PubMed:17720753).
CC {ECO:0000269|PubMed:15269331, ECO:0000269|PubMed:17720753}.
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DR EMBL; U89959; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE27386.1; -; Genomic_DNA.
DR RefSeq; NP_171714.2; NM_100092.3.
DR AlphaFoldDB; F4HVW5; -.
DR SMR; F4HVW5; -.
DR STRING; 3702.AT1G02120.1; -.
DR iPTMnet; F4HVW5; -.
DR PaxDb; F4HVW5; -.
DR PRIDE; F4HVW5; -.
DR ProteomicsDB; 228548; -.
DR EnsemblPlants; AT1G02120.1; AT1G02120.1; AT1G02120.
DR GeneID; 839232; -.
DR Gramene; AT1G02120.1; AT1G02120.1; AT1G02120.
DR KEGG; ath:AT1G02120; -.
DR Araport; AT1G02120; -.
DR TAIR; locus:2205625; AT1G02120.
DR eggNOG; KOG1032; Eukaryota.
DR HOGENOM; CLU_025418_0_0_1; -.
DR InParanoid; F4HVW5; -.
DR OMA; MYMVEAR; -.
DR OrthoDB; 944155at2759; -.
DR PRO; PR:F4HVW5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HVW5; baseline and differential.
DR Genevisible; F4HVW5; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0043069; P:negative regulation of programmed cell death; IMP:UniProtKB.
DR GO; GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.
DR GO; GO:0009723; P:response to ethylene; IMP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IMP:UniProtKB.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR004182; GRAM.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR031968; VASt.
DR Pfam; PF02893; GRAM; 1.
DR Pfam; PF16016; VASt; 1.
DR SMART; SM00568; GRAM; 1.
DR PROSITE; PS51778; VAST; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Chloroplast; Coiled coil; Ethylene signaling pathway;
KW Glycoprotein; Hypersensitive response; Membrane; Plant defense; Plastid;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..68
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 69..598
FT /note="Protein VASCULAR ASSOCIATED DEATH 1, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431742"
FT TRANSMEM 507..527
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 70..134
FT /note="GRAM"
FT /evidence="ECO:0000255"
FT DOMAIN 272..444
FT /note="VASt"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01114"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 553..595
FT /evidence="ECO:0000255"
FT COMPBIAS 43..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 329
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 494
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 598 AA; 67702 MW; 7E41F034786D639C CRC64;
MAMLSTASVS GSVDLPRGTM KVDSSASPEV VSDLPPSSPK GSPDRHDPST SSPSPSRGGD
NQSEVISKSE EYRQLFRLPA DEILVQDFNC ACQESILMQG HMYLFIHYIC FYSNIFGYET
KKIIPFAEIS CVKRAKTAGI FPNAIEILAG GKKYFFASFL SRDEAFKLIH DGWLEYGSAV
KSEGEILVTE PQVSDGVVKR ARSSMDLANE LDIPVRDETL HLSSSSSLPV ISQNGVPPSS
VQRHAEPDVD VVAANTFNWK PEDTDAPKLS SDFTKVAEAK FSIPVEEFFR LFFSDGAVSF
VESFHKNCGD KEFRCTSWQP HEKLGHTRNV SFQHPIKIYF GAKFGGCQES QKFRMYRNSH
LVIETSQEIS DVPYADYFTV EGVWDLKRDC RDSVEGCILD VYVNVAFSKR TVWKGKIVQS
TLEECREAYA HWIRMAHELL KQKKLENQEG NKLIEDGEPL AAREERVSEC DEEGKVEMVG
EGVVKKSLKE AWVNLTSFVK RQSGTRQVIV LAFAVILLMQ VTIVVLLKKG GGGQVEYHER
YDEYSVNGET LGWLEKRMHF LREEMMMVED RLQRMRQDHA ALKAQFHHLE RLLRRNKQ
