ID   VAM10_YEAST             Reviewed;         114 AA.
AC   Q08474; D6W2D2; O00027;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Vacuolar morphogenesis protein 10;
GN   Name=VAM10; OrderedLocusNames=YOR068C; ORFNames=YOR29-19;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9133743;
RX   DOI=10.1002/(sici)1097-0061(19970330)13:4<379::aid-yea85>3.0.co;2-g;
RA   Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.;
RT   "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the
RT   presence of two tRNAs and 24 new open reading frames.";
RL   Yeast 13:379-390(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA   Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA   Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA   Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA   Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA   Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA   Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA   Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA   Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA   Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA   Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA   Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA   Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   FUNCTION.
RX   PubMed=12748377; DOI=10.1073/pnas.1132162100;
RA   Kato M., Wickner W.;
RT   "Vam10p defines a Sec18p-independent step of priming that allows yeast
RT   vacuole tethering.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:6398-6403(2003).
CC   -!- FUNCTION: Required for vacuolar fusion. Involved in the early steps of
CC       the fusion pathway. {ECO:0000269|PubMed:12748377}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000305}; Peripheral
CC       membrane protein {ECO:0000305}.
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DR   EMBL; Z70678; CAA94553.1; -; Genomic_DNA.
DR   EMBL; Z74976; CAA99261.1; -; Genomic_DNA.
DR   EMBL; BK006948; DAA10848.1; -; Genomic_DNA.
DR   PIR; S66951; S66951.
DR   RefSeq; NP_014711.1; NM_001183487.1.
DR   AlphaFoldDB; Q08474; -.
DR   BioGRID; 34467; 302.
DR   DIP; DIP-4164N; -.
DR   STRING; 4932.YOR068C; -.
DR   iPTMnet; Q08474; -.
DR   PaxDb; Q08474; -.
DR   EnsemblFungi; YOR068C_mRNA; YOR068C; YOR068C.
DR   GeneID; 854234; -.
DR   KEGG; sce:YOR068C; -.
DR   SGD; S000005594; VAM10.
DR   VEuPathDB; FungiDB:YOR068C; -.
DR   HOGENOM; CLU_2122470_0_0_1; -.
DR   BioCyc; YEAST:G3O-33607-MON; -.
DR   PRO; PR:Q08474; -.
DR   Proteomes; UP000002311; Chromosome XV.
DR   RNAct; Q08474; protein.
DR   GO; GO:0000329; C:fungal-type vacuole membrane; IC:SGD.
DR   GO; GO:0042144; P:vacuole fusion, non-autophagic; IMP:SGD.
PE   4: Predicted;
KW   Membrane; Reference proteome; Vacuole.
FT   CHAIN           1..114
FT                   /note="Vacuolar morphogenesis protein 10"
FT                   /id="PRO_0000065758"
SQ   SEQUENCE   114 AA;  11764 MW;  C2C7E69630990E57 CRC64;
     MLFEVFGEVL ASYIVSSKTK GELAFPVNNA PPDSLVAINC VVLFLRSAIG SCSGAKELIR
     SSALELSCSS SCGLPATDKP GSFHSGALSK SILSANEAVV SKSSLSFLSS FVDI