VAM6_SCHPO
ID VAM6_SCHPO Reviewed; 905 AA.
AC O13955;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Vacuolar morphogenesis protein 6;
DE AltName: Full=Vacuolar protein sorting-associated protein 39;
GN Name=vam6; Synonyms=vps39; ORFNames=SPAC23H4.14;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Required for vacuolar assembly. Acts as component of the HOPS
CC complex that acts during the docking stage of vacuole fusion. HOPS is
CC required for vacuolar SNARE complex assembly. It remains bound to SNARE
CC complexes after vacuole fusion (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the HOPS complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VAM6/VPS39 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB11668.1; -; Genomic_DNA.
DR PIR; T38314; T38314.
DR RefSeq; NP_593392.1; NM_001018824.2.
DR AlphaFoldDB; O13955; -.
DR BioGRID; 278349; 1.
DR STRING; 4896.SPAC23H4.14.1; -.
DR iPTMnet; O13955; -.
DR MaxQB; O13955; -.
DR PaxDb; O13955; -.
DR PRIDE; O13955; -.
DR EnsemblFungi; SPAC23H4.14.1; SPAC23H4.14.1:pep; SPAC23H4.14.
DR GeneID; 2541859; -.
DR KEGG; spo:SPAC23H4.14; -.
DR PomBase; SPAC23H4.14; vam6.
DR VEuPathDB; FungiDB:SPAC23H4.14; -.
DR eggNOG; KOG2063; Eukaryota.
DR HOGENOM; CLU_004190_1_1_1; -.
DR InParanoid; O13955; -.
DR OMA; DETEMAR; -.
DR PhylomeDB; O13955; -.
DR PRO; PR:O13955; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:PomBase.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:1902500; C:vacuolar HOPS complex; ISO:PomBase.
DR GO; GO:1990816; C:vacuole-mitochondrion membrane contact site; ISO:PomBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IGI:PomBase.
DR GO; GO:0006914; P:autophagy; IBA:GO_Central.
DR GO; GO:0034058; P:endosomal vesicle fusion; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:1904263; P:positive regulation of TORC1 signaling; EXP:PomBase.
DR GO; GO:0097576; P:vacuole fusion; EXP:PomBase.
DR GO; GO:0140057; P:vacuole-mitochondria membrane tethering; TAS:PomBase.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR000547; Clathrin_H-chain/VPS_repeat.
DR InterPro; IPR001180; CNH_dom.
DR InterPro; IPR032914; Vam6/VPS39/TRAP1.
DR InterPro; IPR019452; VPS39/TGF_beta_rcpt-assoc_1.
DR InterPro; IPR019453; VPS39/TGF_beta_rcpt-assoc_2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR12894; PTHR12894; 1.
DR Pfam; PF00637; Clathrin; 1.
DR Pfam; PF00780; CNH; 1.
DR Pfam; PF10366; Vps39_1; 1.
DR Pfam; PF10367; Vps39_2; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50236; CHCR; 1.
DR PROSITE; PS50219; CNH; 1.
PE 3: Inferred from homology;
KW Membrane; Protein transport; Reference proteome; Transport; Vacuole.
FT CHAIN 1..905
FT /note="Vacuolar morphogenesis protein 6"
FT /id="PRO_0000372690"
FT DOMAIN 15..293
FT /note="CNH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00795"
FT REPEAT 614..786
FT /note="CHCR"
SQ SEQUENCE 905 AA; 103696 MW; 8C0FD258D4745D0E CRC64;
MHRAFSLYRV LELSKARVEC VFELGGLVYV SNSNGDLDSY KIYNNEEEEA ADFVMEHVDV
YPNFTKKPIT KVVSCATQDI FYALSDSQVY VYQISTFKKL FSFGAHCQNM CLYGDELIVL
SSKKNLEIYE IQKNSKPNLT KTISLNDRPR SLAWVSPTMI LVSLSNDFCA VNTETSRISS
LNLAWQQSSS LGLGISYIGM SIKSNKLHIT RISDDEVLLS KDSQGLLVNL KSLQVSRNPL
RWPTVPQAVI YNSPYIITLH NQYIYIWNKE TYAMIQQIGI SNIYSTFSCH KNTFFTSNSY
VWILTPEDFS NQIEALLNTE NLNEAISVLS QITVSQFPKR DYYLRITKRE KALRSFSSGD
YDLAMRLFSE ISESPSTVLG LFPGLLDNNY SDAISILSMA PSQNESIESN VLFPGNHSNS
QTDLRNGDAV STVANNKRLR SLSTYLTDSR RKANRFLSYD EEHYFLQKKN LFLNADGTLV
AKEKLEKIAV QIDTTLFLIY MISSPALVGS LLRLPNRCET SVVETNLLSA KMYRELVEYY
YGKSLHEAAL DLLTKLCDEP TDTLSLKGKS NTTSKYEPIL SYLEKLSPEL DHLIFKYSRV
PLSEDPQNSI VIFIDENSEA STISKGVVLK YLETISYKVS IIYLEKLLLD NKFNDTVFPT
RLALLYLKRI LELEETTDFK NQEVFKQTIE KLEDYLTNSK QYDANVVLQE INSQDEFLST
VSIILYRRLS RHQDALDVYL KILNDWEGAL SYCNSVYSID GETEPYYMLL AEISKNYKSG
SLNILDFITK YSSRLDLNRV FPLLPKNISM KSYHSLFSSQ FRQLFEELSN KETQSKLYQK
RLEDLNEELT KVRSEKVVIT REKTCLFCHK RLGKSVISIF PDGSVVHYGC AKKYVSSNHL
PYEAY
