VAM7A_DICDI
ID VAM7A_DICDI Reviewed; 216 AA.
AC Q54NW7;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Vesicle-associated membrane protein 7A;
GN Name=vamp7A {ECO:0000312|EMBL:EAL64939.1}; Synonyms=vamp7;
GN ORFNames=DDB_G0284951;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1] {ECO:0000312|EMBL:EAL64939.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 23-33 AND 72-85, FUNCTION, IDENTIFICATION IN SNARE
RP COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=12175335; DOI=10.1042/bj20020845;
RA Bogdanovic A., Bennett N., Kieffer S., Louwagie M., Morio T., Garin J.,
RA Satre M., Bruckert F.;
RT "Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane
RT protein 7) form an active SNARE complex for early macropinocytic
RT compartment fusion in Dictyostelium discoideum.";
RL Biochem. J. 368:29-39(2002).
CC -!- FUNCTION: Involved in the targeting and/or fusion of transport vesicles
CC to their target membrane during transport of proteins from the early
CC endosome to the lysosome. Required for heterotypic fusion of late
CC endosomes with lysosomes and homotypic lysosomal fusion. Required for
CC calcium regulated lysosomal exocytosis. {ECO:0000250|UniProtKB:P51809,
CC ECO:0000269|PubMed:12175335}.
CC -!- SUBUNIT: Component of the SNARE complex composed of syn7A, syn8A,
CC vamp7A and vti1A. {ECO:0000269|PubMed:12175335}.
CC -!- INTERACTION:
CC Q54NW7; Q54JY7: syn7A; NbExp=6; IntAct=EBI-1810311, EBI-1810238;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle membrane
CC {ECO:0000269|PubMed:12175335}; Single-pass type IV membrane protein
CC {ECO:0000269|PubMed:12175335}. Golgi apparatus, trans-Golgi network
CC membrane {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}; Single-pass type IV membrane protein
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}. Late endosome membrane {ECO:0000269|PubMed:12175335};
CC Single-pass type IV membrane protein {ECO:0000269|PubMed:12175335}.
CC Lysosome membrane {ECO:0000269|PubMed:12175335}; Single-pass type IV
CC membrane protein {ECO:0000269|PubMed:12175335}. Endoplasmic reticulum
CC membrane {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}; Single-pass type IV membrane protein
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}. Cytoplasmic vesicle, phagosome membrane
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}; Single-pass type IV membrane protein
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}. Note=During macropinocytosis highest levels are found in
CC endosomes with lower levels observed in the lysosomes.
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255, ECO:0000269|PubMed:12175335}.
CC -!- SIMILARITY: Belongs to the synaptobrevin family. {ECO:0000305}.
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DR EMBL; AAFI02000073; EAL64939.1; -; Genomic_DNA.
DR RefSeq; XP_639950.1; XM_634858.1.
DR AlphaFoldDB; Q54NW7; -.
DR SMR; Q54NW7; -.
DR IntAct; Q54NW7; 1.
DR STRING; 44689.DDB0231535; -.
DR PaxDb; Q54NW7; -.
DR EnsemblProtists; EAL64939; EAL64939; DDB_G0284951.
DR GeneID; 8624862; -.
DR KEGG; ddi:DDB_G0284951; -.
DR dictyBase; DDB_G0284951; vamp7A.
DR eggNOG; KOG0859; Eukaryota.
DR HOGENOM; CLU_064620_1_1_1; -.
DR InParanoid; Q54NW7; -.
DR OMA; SKFFIHC; -.
DR PhylomeDB; Q54NW7; -.
DR Reactome; R-DDI-199992; trans-Golgi Network Vesicle Budding.
DR PRO; PR:Q54NW7; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0030666; C:endocytic vesicle membrane; IDA:dictyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0005768; C:endosome; IDA:dictyBase.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IDA:UniProtKB.
DR GO; GO:0005765; C:lysosomal membrane; IDA:UniProtKB.
DR GO; GO:0045335; C:phagocytic vesicle; IDA:dictyBase.
DR GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031201; C:SNARE complex; IDA:UniProtKB.
DR GO; GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008333; P:endosome to lysosome transport; IEP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006906; P:vesicle fusion; IDA:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; ISS:UniProtKB.
DR CDD; cd14824; Longin; 1.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR010908; Longin_dom.
DR InterPro; IPR001388; Synaptobrevin.
DR InterPro; IPR042855; V_SNARE_CC.
DR Pfam; PF13774; Longin; 1.
DR Pfam; PF00957; Synaptobrevin; 1.
DR PRINTS; PR00219; SYNAPTOBREVN.
DR SMART; SM01270; Longin; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS50859; LONGIN; 1.
DR PROSITE; PS50892; V_SNARE; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasmic vesicle; Direct protein sequencing;
KW Endoplasmic reticulum; Endosome; Golgi apparatus; Lysosome; Membrane;
KW Protein transport; Reference proteome; Signal-anchor; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..216
FT /note="Vesicle-associated membrane protein 7A"
FT /id="PRO_0000320001"
FT TOPO_DOM 1..189
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Anchor for type IV membrane protein"
FT TOPO_DOM 211..216
FT /note="Vesicular"
FT /evidence="ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255"
FT DOMAIN 6..112
FT /note="Longin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00231"
FT DOMAIN 126..186
FT /note="v-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00290"
SQ SEQUENCE 216 AA; 24772 MW; C8F7FA4F5EEC0143 CRC64;
MSQTDILYAC VSYKGVCLVE HKIANGNFID LARRLITKIP PTSKKIYTSE NHNFHYISEN
DLAFLCLCHE KLGVQIPSEF LSDIRQQFIR SYGQSFSQNS PTATYDPFIR VLEERMKYYS
NPKSNKMNLV MDQVSEAKGA LTDAIEKTIH RGEKIEIIVD KTERLQSESF VFKSNSVALK
RKLWWQNKKL AIAIGLVVCI LIAVITLALL KYFKVI
