VAM7B_DICDI
ID VAM7B_DICDI Reviewed; 260 AA.
AC Q86AQ7; Q550B2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Vesicle-associated membrane protein 7B;
GN Name=vamp7B {ECO:0000312|EMBL:EAL68772.1}; ORFNames=DDB_G0277173;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2] {ECO:0000312|EMBL:EAL68772.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4 {ECO:0000312|EMBL:EAL68772.1};
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Involved in the targeting and/or fusion of transport vesicles
CC to their target membrane during transport of proteins from the early
CC endosome to the lysosome. Required for heterotypic fusion of late
CC endosomes with lysosomes and homotypic lysosomal fusion (By
CC similarity). {ECO:0000250|UniProtKB:Q54NW7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle membrane
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q54NW7,
CC ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255}; Single-pass type IV
CC membrane protein {ECO:0000250|UniProtKB:P70280,
CC ECO:0000250|UniProtKB:Q54NW7, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}. Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q54NW7,
CC ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255}; Single-pass type IV
CC membrane protein {ECO:0000250|UniProtKB:P70280,
CC ECO:0000250|UniProtKB:Q54NW7, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}. Late endosome membrane; Single-pass type IV membrane
CC protein. Lysosome membrane {ECO:0000250|UniProtKB:P70280,
CC ECO:0000250|UniProtKB:Q54NW7, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}; Single-pass type IV membrane protein
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q54NW7,
CC ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255}. Endoplasmic reticulum
CC membrane {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q54NW7,
CC ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255}; Single-pass type IV
CC membrane protein {ECO:0000250|UniProtKB:P70280,
CC ECO:0000250|UniProtKB:Q54NW7, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}. Cytoplasmic vesicle, phagosome membrane
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q54NW7,
CC ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255}; Single-pass type IV
CC membrane protein {ECO:0000250|UniProtKB:P70280,
CC ECO:0000250|UniProtKB:Q54NW7, ECO:0000250|UniProtKB:Q9JHW5,
CC ECO:0000255}. Note=During macropinocytosis highest levels are found in
CC endosomes with lower levels observed in the lysosomes.
CC {ECO:0000250|UniProtKB:P70280, ECO:0000250|UniProtKB:Q54NW7,
CC ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255}.
CC -!- SIMILARITY: Belongs to the synaptobrevin family. {ECO:0000305}.
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DR EMBL; AAFI02000019; EAL68772.1; -; Genomic_DNA.
DR RefSeq; XP_642704.1; XM_637612.1.
DR AlphaFoldDB; Q86AQ7; -.
DR SMR; Q86AQ7; -.
DR STRING; 44689.DDB0231542; -.
DR PaxDb; Q86AQ7; -.
DR EnsemblProtists; EAL68772; EAL68772; DDB_G0277173.
DR GeneID; 8620895; -.
DR KEGG; ddi:DDB_G0277173; -.
DR dictyBase; DDB_G0277173; vamp7B.
DR eggNOG; KOG0859; Eukaryota.
DR HOGENOM; CLU_064620_1_1_1; -.
DR InParanoid; Q86AQ7; -.
DR OMA; NTKLMIM; -.
DR PhylomeDB; Q86AQ7; -.
DR Reactome; R-DDI-199992; trans-Golgi Network Vesicle Budding.
DR PRO; PR:Q86AQ7; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0000331; C:contractile vacuole; IDA:dictyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0005768; C:endosome; IDA:dictyBase.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; ISS:UniProtKB.
DR GO; GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
DR GO; GO:0140220; C:pathogen-containing vacuole; HDA:dictyBase.
DR GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031201; C:SNARE complex; ISS:UniProtKB.
DR GO; GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008333; P:endosome to lysosome transport; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006906; P:vesicle fusion; ISS:UniProtKB.
DR CDD; cd14824; Longin; 1.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR010908; Longin_dom.
DR InterPro; IPR001388; Synaptobrevin.
DR InterPro; IPR042855; V_SNARE_CC.
DR Pfam; PF13774; Longin; 1.
DR Pfam; PF00957; Synaptobrevin; 1.
DR PRINTS; PR00219; SYNAPTOBREVN.
DR SMART; SM01270; Longin; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS50859; LONGIN; 1.
DR PROSITE; PS50892; V_SNARE; 1.
PE 3: Inferred from homology;
KW Coiled coil; Cytoplasmic vesicle; Endoplasmic reticulum; Endosome;
KW Golgi apparatus; Lysosome; Membrane; Protein transport; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..260
FT /note="Vesicle-associated membrane protein 7B"
FT /id="PRO_0000320002"
FT TOPO_DOM 1..189
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Anchor for type IV membrane protein"
FT TOPO_DOM 211..260
FT /note="Vesicular"
FT /evidence="ECO:0000250|UniProtKB:Q9JHW5, ECO:0000255"
FT DOMAIN 7..110
FT /note="Longin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00231"
FT DOMAIN 125..186
FT /note="v-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00290"
FT REGION 215..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..229
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..246
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 260 AA; 29561 MW; CC6EBBBCA94213DF CRC64;
MPIIYSLVAR GSSVLAEFTS TNGNFVTITR RILDLIPPND TKMSYVYEKY IFHYLVSDTL
TYLCMADEEF GRRIPFTFLD DVKNRFKSMY GDKGKTAIAY GMNSDFSRTL ENLMDHYSNT
TRVDTMSRTM AEIDEVKNIL VSDIAPQLLK RGEKIEMLVE RTDTLNQQSF KFKKQSKQLK
CAMWWKNVKL MLVLGAIVLI IIFIIVMSYC DGFRSGSKCR SSPSSNSTPT PTPTETPTPT
PTPTSTPTPS QLLETLLNQF
