ID   VAMP3_BOVIN             Reviewed;         104 AA.
AC   Q2KJD2;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Vesicle-associated membrane protein 3;
DE            Short=VAMP-3;
DE   AltName: Full=Synaptobrevin-3;
GN   Name=VAMP3; Synonyms=SYB3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Heart ventricle;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: SNARE involved in vesicular transport from the late endosomes
CC       to the trans-Golgi network. {ECO:0000250|UniProtKB:Q15836}.
CC   -!- SUBUNIT: Interacts with BVES (via the C-terminus cytoplasmic tail).
CC       Interacts with BCAP31; involved in VAMP3 export from the endoplasmic
CC       reticulum (By similarity). Interacts with BAIAP3; this interaction is
CC       increased in the presence of calcium (By similarity). Interacts with
CC       PICALM (By similarity). {ECO:0000250|UniProtKB:P63024,
CC       ECO:0000250|UniProtKB:Q15836}.
CC   -!- SUBCELLULAR LOCATION: Early endosome membrane
CC       {ECO:0000250|UniProtKB:Q15836}; Single-pass type IV membrane protein
CC       {ECO:0000255}. Recycling endosome membrane
CC       {ECO:0000250|UniProtKB:Q15836}; Single-pass type I membrane protein
CC       {ECO:0000255}. Synapse, synaptosome {ECO:0000250|UniProtKB:Q15836}.
CC   -!- PTM: Ubiquitinated by RNF167 at Lys-70, Lys-72 and Lys-81, regulating
CC       the recycling endosome pathway. {ECO:0000250|UniProtKB:Q15836}.
CC   -!- SIMILARITY: Belongs to the synaptobrevin family. {ECO:0000305}.
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DR   EMBL; BC105399; AAI05400.1; -; mRNA.
DR   RefSeq; NP_001039380.1; NM_001045915.2.
DR   AlphaFoldDB; Q2KJD2; -.
DR   SMR; Q2KJD2; -.
DR   STRING; 9913.ENSBTAP00000019438; -.
DR   PaxDb; Q2KJD2; -.
DR   PRIDE; Q2KJD2; -.
DR   GeneID; 505379; -.
DR   KEGG; bta:505379; -.
DR   CTD; 9341; -.
DR   eggNOG; KOG0860; Eukaryota.
DR   HOGENOM; CLU_064620_4_1_1; -.
DR   InParanoid; Q2KJD2; -.
DR   OrthoDB; 1614157at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0043005; C:neuron projection; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR   GO; GO:0045202; C:synapse; IEA:UniProtKB-SubCell.
DR   GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR   GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR   GO; GO:0017075; F:syntaxin-1 binding; IBA:GO_Central.
DR   GO; GO:0001921; P:positive regulation of receptor recycling; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISS:UniProtKB.
DR   GO; GO:0035493; P:SNARE complex assembly; IBA:GO_Central.
DR   GO; GO:0034446; P:substrate adhesion-dependent cell spreading; ISS:UniProtKB.
DR   GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; ISS:UniProtKB.
DR   InterPro; IPR001388; Synaptobrevin.
DR   InterPro; IPR016444; Synaptobrevin/VAMP.
DR   InterPro; IPR042855; V_SNARE_CC.
DR   PANTHER; PTHR45701; PTHR45701; 1.
DR   Pfam; PF00957; Synaptobrevin; 1.
DR   PIRSF; PIRSF005409; Synaptobrevin_euk; 1.
DR   PRINTS; PR00219; SYNAPTOBREVN.
DR   PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR   PROSITE; PS50892; V_SNARE; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Endosome; Isopeptide bond; Membrane; Protein transport;
KW   Reference proteome; Synapse; Synaptosome; Transmembrane;
KW   Transmembrane helix; Transport; Ubl conjugation.
FT   CHAIN           1..104
FT                   /note="Vesicle-associated membrane protein 3"
FT                   /id="PRO_0000273715"
FT   TOPO_DOM        1..81
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        82..102
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        103..104
FT                   /note="Vesicular"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          18..78
FT                   /note="v-SNARE coiled-coil homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00290"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CROSSLNK        70
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0000250|UniProtKB:Q15836"
FT   CROSSLNK        72
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0000250|UniProtKB:Q15836"
FT   CROSSLNK        81
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0000250|UniProtKB:Q15836"
SQ   SEQUENCE   104 AA;  11535 MW;  5A45A87193513CC8 CRC64;
     MTTNAPAGSS AAAGSSRRLQ QTQNQVDEVV DIMRVNVDKV LERDQKLSEL DDRADALQAG
     ASQFETSAAK LKRKYWWKNC KMWAIGITVV VIIIIIIVVW SISS