ID   VAMP4_BOVIN             Reviewed;         141 AA.
AC   Q32L97; A5PK89;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Vesicle-associated membrane protein 4;
DE            Short=VAMP-4;
GN   Name=VAMP4;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus, and Hereford; TISSUE=Hypothalamus, and Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the pathway that functions to remove an inhibitor
CC       (probably synaptotagmin-4) of calcium-triggered exocytosis during the
CC       maturation of secretory granules. May be a marker for this sorting
CC       pathway that is critical for remodeling the secretory response of
CC       granule (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Identified in a complex containing STX6, STX12, VAMP4 and
CC       VTI1A (By similarity). Interacts with BAIAP3; this interaction is
CC       increased in the presence of calcium (By similarity).
CC       {ECO:0000250|UniProtKB:O70480, ECO:0000250|UniProtKB:O75379}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC       {ECO:0000305}; Single-pass type IV membrane protein {ECO:0000305}.
CC       Note=Associated with trans Golgi network (TGN) and newly formed
CC       immature secretory granules (ISG). Not found on the mature secretory
CC       organelles (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the synaptobrevin family. {ECO:0000305}.
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DR   EMBL; BC109690; AAI09691.1; -; mRNA.
DR   EMBL; BC142401; AAI42402.1; -; mRNA.
DR   RefSeq; NP_001069906.1; NM_001076438.2.
DR   RefSeq; XP_005216973.1; XM_005216916.2.
DR   RefSeq; XP_010811531.1; XM_010813229.2.
DR   AlphaFoldDB; Q32L97; -.
DR   SMR; Q32L97; -.
DR   STRING; 9913.ENSBTAP00000027435; -.
DR   PaxDb; Q32L97; -.
DR   PRIDE; Q32L97; -.
DR   Ensembl; ENSBTAT00000027435; ENSBTAP00000027435; ENSBTAG00000020591.
DR   Ensembl; ENSBTAT00000069950; ENSBTAP00000073260; ENSBTAG00000020591.
DR   Ensembl; ENSBTAT00000071023; ENSBTAP00000069106; ENSBTAG00000020591.
DR   GeneID; 616923; -.
DR   KEGG; bta:616923; -.
DR   CTD; 8674; -.
DR   VEuPathDB; HostDB:ENSBTAG00000020591; -.
DR   VGNC; VGNC:36758; VAMP4.
DR   eggNOG; KOG0860; Eukaryota.
DR   GeneTree; ENSGT00940000155005; -.
DR   HOGENOM; CLU_149550_0_0_1; -.
DR   InParanoid; Q32L97; -.
DR   OMA; WWRGCKV; -.
DR   OrthoDB; 1614157at2759; -.
DR   TreeFam; TF313666; -.
DR   Reactome; R-BTA-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR   Proteomes; UP000009136; Chromosome 16.
DR   Bgee; ENSBTAG00000020591; Expressed in occipital lobe and 105 other tissues.
DR   GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR   GO; GO:0008021; C:synaptic vesicle; IBA:GO_Central.
DR   GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR   GO; GO:0090161; P:Golgi ribbon formation; IBA:GO_Central.
DR   GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0035493; P:SNARE complex assembly; IBA:GO_Central.
DR   InterPro; IPR001388; Synaptobrevin.
DR   InterPro; IPR042855; V_SNARE_CC.
DR   InterPro; IPR042887; VAMP4.
DR   PANTHER; PTHR46897; PTHR46897; 1.
DR   Pfam; PF00957; Synaptobrevin; 1.
DR   PRINTS; PR00219; SYNAPTOBREVN.
DR   PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR   PROSITE; PS50892; V_SNARE; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Golgi apparatus; Membrane; Phosphoprotein; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..141
FT                   /note="Vesicle-associated membrane protein 4"
FT                   /id="PRO_0000273716"
FT   TOPO_DOM        1..115
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        137..141
FT                   /note="Vesicular"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          52..112
FT                   /note="v-SNARE coiled-coil homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00290"
FT   REGION          1..51
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..26
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         17
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O70480"
FT   MOD_RES         30
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O75379"
SQ   SEQUENCE   141 AA;  16393 MW;  4DDB9682C63CF91E CRC64;
     MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV
     DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKIKAIM
     ALVAVILLLV IIILIVVKYR T