VAMP5_MOUSE
ID VAMP5_MOUSE Reviewed; 102 AA.
AC Q9Z2P8;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Vesicle-associated membrane protein 5;
DE Short=VAMP-5;
DE AltName: Full=Myobrevin;
GN Name=Vamp5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9725904; DOI=10.1091/mbc.9.9.2423;
RA Zeng Q., Subramaniam V.N., Wong S.H., Tang B.L., Parton R.G., Rea S.,
RA James D.E., Hong W.;
RT "A novel synaptobrevin/VAMP homologous protein (VAMP5) is increased during
RT in vitro myogenesis and present in the plasma membrane.";
RL Mol. Biol. Cell 9:2423-2437(1998).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May participate in trafficking events that are associated
CC with myogenesis, such as myoblast fusion and/or GLUT4 trafficking.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type IV
CC membrane protein {ECO:0000305}. Endomembrane system {ECO:0000305};
CC Single-pass type IV membrane protein {ECO:0000305}. Golgi apparatus,
CC trans-Golgi network membrane {ECO:0000305}; Single-pass type IV
CC membrane protein {ECO:0000305}. Note=Associated with the plasma
CC membrane as well as intracellular perinuclear and peripheral vesicular
CC structures of myotubes. Associated with the trans-Golgi, but not with
CC the cis-Golgi apparatus.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in the skeletal muscle and
CC heart, detected at lower levels in several other tissues but not in the
CC brain.
CC -!- INDUCTION: During myogenesis.
CC -!- SIMILARITY: Belongs to the synaptobrevin family. {ECO:0000305}.
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DR EMBL; AF035643; AAC95282.1; -; mRNA.
DR CCDS; CCDS39513.1; -.
DR RefSeq; NP_001074211.1; NM_001080742.2.
DR RefSeq; NP_058568.1; NM_016872.4.
DR AlphaFoldDB; Q9Z2P8; -.
DR SMR; Q9Z2P8; -.
DR BioGRID; 207337; 2.
DR IntAct; Q9Z2P8; 1.
DR STRING; 10090.ENSMUSP00000098843; -.
DR iPTMnet; Q9Z2P8; -.
DR PhosphoSitePlus; Q9Z2P8; -.
DR SwissPalm; Q9Z2P8; -.
DR jPOST; Q9Z2P8; -.
DR MaxQB; Q9Z2P8; -.
DR PaxDb; Q9Z2P8; -.
DR PRIDE; Q9Z2P8; -.
DR ProteomicsDB; 300112; -.
DR TopDownProteomics; Q9Z2P8; -.
DR Antibodypedia; 31895; 175 antibodies from 25 providers.
DR DNASU; 53620; -.
DR Ensembl; ENSMUST00000074231; ENSMUSP00000073852; ENSMUSG00000073002.
DR Ensembl; ENSMUST00000101285; ENSMUSP00000098843; ENSMUSG00000073002.
DR GeneID; 53620; -.
DR KEGG; mmu:53620; -.
DR UCSC; uc009cil.2; mouse.
DR CTD; 10791; -.
DR MGI; MGI:1858622; Vamp5.
DR VEuPathDB; HostDB:ENSMUSG00000073002; -.
DR eggNOG; KOG0860; Eukaryota.
DR GeneTree; ENSGT00730000111371; -.
DR HOGENOM; CLU_064620_4_2_1; -.
DR InParanoid; Q9Z2P8; -.
DR OMA; VRCRIYL; -.
DR OrthoDB; 1607213at2759; -.
DR PhylomeDB; Q9Z2P8; -.
DR TreeFam; TF313666; -.
DR BioGRID-ORCS; 53620; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q9Z2P8; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9Z2P8; protein.
DR Bgee; ENSMUSG00000073002; Expressed in granulocyte and 166 other tissues.
DR ExpressionAtlas; Q9Z2P8; baseline and differential.
DR Genevisible; Q9Z2P8; MM.
DR GO; GO:0009986; C:cell surface; ISO:MGI.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IDA:BHF-UCL.
DR GO; GO:0031301; C:integral component of organelle membrane; IDA:BHF-UCL.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:MGI.
DR GO; GO:0014704; C:intercalated disc; IDA:BHF-UCL.
DR GO; GO:0005770; C:late endosome; IDA:BHF-UCL.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0005802; C:trans-Golgi network; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IMP:MGI.
DR GO; GO:0007519; P:skeletal muscle tissue development; IDA:MGI.
DR CDD; cd15872; R-SNARE_VAMP5; 1.
DR InterPro; IPR001388; Synaptobrevin.
DR InterPro; IPR016444; Synaptobrevin/VAMP.
DR InterPro; IPR042855; V_SNARE_CC.
DR InterPro; IPR042166; Vamp5.
DR InterPro; IPR042581; VAMP5_R-SNARE.
DR PANTHER; PTHR47462; PTHR47462; 1.
DR Pfam; PF00957; Synaptobrevin; 1.
DR PIRSF; PIRSF005409; Synaptobrevin_euk; 1.
DR PRINTS; PR00219; SYNAPTOBREVN.
DR PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR PROSITE; PS50892; V_SNARE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Coiled coil; Developmental protein; Differentiation;
KW Golgi apparatus; Membrane; Myogenesis; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..102
FT /note="Vesicle-associated membrane protein 5"
FT /id="PRO_0000206734"
FT TOPO_DOM 1..72
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..102
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT DOMAIN 5..65
FT /note="v-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00290"
FT MOD_RES 41
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 48
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O95183"
FT MOD_RES 49
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O95183"
SQ SEQUENCE 102 AA; 11416 MW; 37707734244C5DC7 CRC64;
MAGKELKQCQ QQADEVTEIM LNNFDKVLER HGKLAELEQR SDQLLDMSSA FSKTTKTLAQ
QKRWENIRCR VYLGLAVAVG LLIILIVLLV VFLPSGGDSS KP
