ID   VANR_ENTFA              Reviewed;         220 AA.
AC   Q47744;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Regulatory protein VanRB;
GN   Name=vanRB; OrderedLocusNames=EF_2299;
OS   Enterococcus faecalis (strain ATCC 700802 / V583).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=226185;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=8631706; DOI=10.1128/jb.178.5.1302-1309.1996;
RA   Evers S., Courvalin P.;
RT   "Regulation of VanB-type vancomycin resistance gene expression by the
RT   VanS(B)-VanR(B) two-component regulatory system in Enterococcus faecalis
RT   V583.";
RL   J. Bacteriol. 178:1302-1309(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA   Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA   DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA   Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA   Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT   faecalis.";
RL   Science 299:2071-2074(2003).
CC   -!- FUNCTION: Member of the two-component regulatory system VanS/VanR.
CC       Activates the transcription of vanH, vanA and vanX in response to
CC       vancomycin which results in vancomycin resistance.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by VanS. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U35369; AAB05622.1; -; Genomic_DNA.
DR   EMBL; AE016830; AAO82026.1; -; Genomic_DNA.
DR   RefSeq; NP_815956.1; NC_004668.1.
DR   RefSeq; WP_002368697.1; NZ_KE136528.1.
DR   AlphaFoldDB; Q47744; -.
DR   SMR; Q47744; -.
DR   STRING; 226185.EF_2299; -.
DR   EnsemblBacteria; AAO82026; AAO82026; EF_2299.
DR   KEGG; efa:EF2299; -.
DR   PATRIC; fig|226185.45.peg.1233; -.
DR   eggNOG; COG0745; Bacteria.
DR   HOGENOM; CLU_000445_30_3_9; -.
DR   OMA; YFGETRI; -.
DR   Proteomes; UP000001415; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Antibiotic resistance; Cell wall biogenesis/degradation;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Repressor;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..220
FT                   /note="Regulatory protein VanRB"
FT                   /id="PRO_0000081262"
FT   DOMAIN          4..117
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        124..218
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         53
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   220 AA;  25336 MW;  8085827C1DEF7AB8 CRC64;
     MSIRILLVED DDHICNTVRA FLAEARYEVD ACTDGNEAHT KFYENTYQLV ILDIMLPGMN
     GHELLREFRA QNDTPILMMT ALSDDENQIR AFDAEADDYV TKPFKMRILL KRVEALLRRS
     GALAKEFRVG RLTLLPEDFR VLCDGTELPL TRKEFEILLL LVQNKGRTLT HEIILSRIWG
     YDFDGDGSTV HTHIKNLRAK LPENIIKTIR GVGYRLEESL