ID   VANR_ENTFC              Reviewed;         231 AA.
AC   Q06239;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Regulatory protein VanR;
GN   Name=vanR;
OS   Enterococcus faecium (Streptococcus faecium).
OG   Plasmid pIP816, and Plasmid pHKK100.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1352;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BM4147; PLASMID=pIP816; TRANSPOSON=Tn1546;
RX   PubMed=8380148; DOI=10.1128/jb.175.1.117-127.1993;
RA   Arthur M., Molinas C., Depardieu F., Courvalin P.;
RT   "Characterization of Tn1546, a Tn3-related transposon conferring
RT   glycopeptide resistance by synthesis of depsipeptide peptidoglycan
RT   precursors in Enterococcus faecium BM4147.";
RL   J. Bacteriol. 175:117-127(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BM4147; PLASMID=pIP816;
RX   PubMed=1556077; DOI=10.1128/jb.174.8.2582-2591.1992;
RA   Arthur M., Molinas C., Courvalin P.;
RT   "The VanS-VanR two-component regulatory system controls synthesis of
RT   depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147.";
RL   J. Bacteriol. 174:2582-2591(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=221; PLASMID=pHKK100;
RX   PubMed=1320585; DOI=10.1016/0378-1097(92)90533-t;
RA   Handwerger S., Discotto L., Thanassi J., Pucci M.J.;
RT   "Insertional inactivation of a gene which controls expression of vancomycin
RT   resistance on plasmid pHKK100.";
RL   FEMS Microbiol. Lett. 71:11-14(1992).
CC   -!- FUNCTION: Member of the two-component regulatory system VanS/VanR.
CC       Activates the transcription of vanH, vanA and vanX in response to
CC       vancomycin which results in vancomycin resistance.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by VanS. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M97297; AAA65953.1; -; Genomic_DNA.
DR   EMBL; M68910; AAA24787.1; -; Genomic_DNA.
DR   EMBL; M84146; AAA24790.1; -; Genomic_DNA.
DR   PIR; A41838; A41838.
DR   RefSeq; WP_001280781.1; NZ_WSZC01000102.1.
DR   RefSeq; YP_001019038.1; NC_008821.1.
DR   RefSeq; YP_001974799.1; NC_010980.1.
DR   RefSeq; YP_002128396.1; NC_011140.1.
DR   RefSeq; YP_004172619.1; NC_014959.1.
DR   RefSeq; YP_006937552.1; NC_013317.1.
DR   RefSeq; YP_976080.1; NC_008768.1.
DR   AlphaFoldDB; Q06239; -.
DR   SMR; Q06239; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IGI:CACAO.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Antibiotic resistance; Cell wall biogenesis/degradation;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Plasmid; Repressor; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..231
FT                   /note="Regulatory protein VanR"
FT                   /id="PRO_0000081263"
FT   DOMAIN          4..117
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        131..231
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         53
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   231 AA;  26643 MW;  56A9925CC2254FFC CRC64;
     MSDKILIVDD EHEIADLVEL YLKNENYTVF KYYTAKEALE CIDKSEIDLA ILDIMLPGTS
     GLTICQKIRD KHTYPIIMLT GKDTEVDKIT GLTIGADDYI TKPFRPLELI ARVKAQLRRY
     KKFSGVKEQN ENVIVHSGLV INVNTHECYL NEKQLSLTPT EFSILRILCE NKGNVVSSEL
     LFHEIWGDEY FSKSNNTITV HIRHLREKMN DTIDNPKYIK TVWGVGYKIE K