ID   VAO_PLEOS               Reviewed;          28 AA.
AC   P21800;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Veratryl alcohol oxidase;
DE            Short=VAO;
DE            EC=1.1.3.-;
DE   Flags: Fragments;
OS   Pleurotus ostreatus (Oyster mushroom) (White-rot fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Pleurotaceae; Pleurotus.
OX   NCBI_TaxID=5322;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=Florida;
RX   PubMed=1991127; DOI=10.1016/0304-4165(91)90190-r;
RA   Sannia G., Limongi P., Cocca E., Buonocore F., Nitti G., Giardina P.;
RT   "Purification and characterization of a veratryl alcohol oxidase enzyme
RT   from the lignin degrading basidiomycete Pleurotus ostreatus.";
RL   Biochim. Biophys. Acta 1073:114-119(1991).
CC   -!- FUNCTION: Plays a central role in the biodegradation of lignin.
CC       Oxidizes veratryl alcohol to veratraldehyde reducing O(2) to H(2)O(2).
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Moderately thermostable. Has a 1.5 hour half-life at 55 degrees
CC         Celsius.;
CC   -!- MISCELLANEOUS: Primary aromatic alcohols with methoxy substituents in
CC       position four are good substrates for VAO.
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DR   PIR; S13664; S13664.
DR   AlphaFoldDB; P21800; -.
DR   SMR; P21800; -.
DR   BioCyc; MetaCyc:MON-17610; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046274; P:lignin catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; FAD; Flavoprotein; Lignin degradation;
KW   Oxidoreductase.
FT   CHAIN           1..28
FT                   /note="Veratryl alcohol oxidase"
FT                   /id="PRO_0000065764"
FT   VARIANT         1..3
FT                   /note="Missing (in 60-70% of the mature VAO)"
FT   NON_CONS        23..24
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   28 AA;  2910 MW;  2363DAD466FC8920 CRC64;
     KPTADFDYIV VGAGNAGNVV AYRTVQAL