VAP14_ARATH
ID VAP14_ARATH Reviewed; 571 AA.
AC Q9SYC9;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Vesicle-associated protein 1-4;
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Plant VAP homolog 14;
DE Short=AtPVA14;
DE AltName: Full=VAMP-associated protein 1-4;
GN Name=PVA14; OrderedLocusNames=At1g51270; ORFNames=F11M15.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 333-571.
RA Wrobel R.L., Kimball T.L., Riters M.A., Steffen E., Thao S., Aceti D.J.,
RA Blommel P.G., Newman C.S., Zhao Q., Fox B.G., Phillips G.N. Jr.,
RA Markley J.L.;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May play a role in vesicle trafficking. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9SYC9-1; Sequence=Displayed;
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- SIMILARITY: Belongs to the VAMP-associated protein (VAP) (TC 9.B.17)
CC family. {ECO:0000305}.
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DR EMBL; AC006085; AAD30639.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32644.1; -; Genomic_DNA.
DR EMBL; BT015497; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; E96550; E96550.
DR RefSeq; NP_175538.3; NM_104005.3. [Q9SYC9-1]
DR AlphaFoldDB; Q9SYC9; -.
DR SMR; Q9SYC9; -.
DR STRING; 3702.AT1G51270.3; -.
DR PaxDb; Q9SYC9; -.
DR PRIDE; Q9SYC9; -.
DR EnsemblPlants; AT1G51270.1; AT1G51270.1; AT1G51270. [Q9SYC9-1]
DR GeneID; 841550; -.
DR Gramene; AT1G51270.1; AT1G51270.1; AT1G51270. [Q9SYC9-1]
DR KEGG; ath:AT1G51270; -.
DR Araport; AT1G51270; -.
DR eggNOG; KOG0439; Eukaryota.
DR InParanoid; Q9SYC9; -.
DR PRO; PR:Q9SYC9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SYC9; baseline and differential.
DR Genevisible; Q9SYC9; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0090158; P:endoplasmic reticulum membrane organization; IBA:GO_Central.
DR GO; GO:0061817; P:endoplasmic reticulum-plasma membrane tethering; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 2.60.40.10; -; 2.
DR Gene3D; 3.40.50.10140; -; 1.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR000535; MSP_dom.
DR InterPro; IPR008962; PapD-like_sf.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR InterPro; IPR016763; VAP.
DR PANTHER; PTHR10809; PTHR10809; 2.
DR Pfam; PF00635; Motile_Sperm; 2.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF49354; SSF49354; 2.
DR SUPFAM; SSF52200; SSF52200; 1.
DR PROSITE; PS50202; MSP; 2.
DR PROSITE; PS50104; TIR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; NAD; Reference proteome; Repeat.
FT CHAIN 1..571
FT /note="Vesicle-associated protein 1-4"
FT /id="PRO_0000402172"
FT DOMAIN 1..126
FT /note="MSP 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00132"
FT DOMAIN 176..296
FT /note="MSP 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00132"
FT DOMAIN 356..493
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT REGION 132..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 136..153
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 306..322
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 430
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT CONFLICT 372
FT /note="S -> G (in Ref. 3; BT015497)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 571 AA; 64407 MW; 61B6C9F20696FFE7 CRC64;
MSTDELLTFD HVDIRFPIEL NKQGSCSLNL TNKTDNYVAF KAQTTKPKMY CVKPSVGVVL
PRSSCEVLVV MQALKEAPAD RQCKDKLLFQ CKVVEPGTMD KEVTSEMFSK EAGHRVEETI
FKIIYVAPPQ PQSPVQEGLE DGSSPSASVS DKGNASEVFV GPSVGIVDLI RMSDELLIID
PVDVQFPIEL NKKVSCSLNL TNKTENYVAF KAKTTNAKKY YVRPNVGVVL PRSSCEVLVI
MQALKEAPAD MQCRDKLLFQ CKVVEPETTA KDVTSEMFSK EAGHPAEETR LKVMYVTPPQ
PPSPVQEGTE EGSSPRASVS DNGNASEAFV DMLRSLLVPL FSNAASSTDD HGITLPQYQV
FINFRGDELR NSFVGFLVKA MRLEKINVFT DEVELRGTNL NYLFRRIEES RVAVAIFSER
YTESCWCLDE LVKMKEQMEQ GKLVVVPVFY RLNATACKRF MGAFGDNLRN LEWEYRSEPE
RIQKWKEALS SVFSNIGLTS DIRRYNLINK NMDHTSEFLY IVLILNFFSE ISDMTGLTTS
YQFLLMMKSN LISYDIYIYP TKFCVNVFIG V
