ID   VAPB1_HAEI6             Reviewed;          78 AA.
AC   E4QWH3;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Antitoxin VapB1;
GN   Name=vapB1; OrderedLocusNames=R2866_0252;
OS   Haemophilus influenzae (strain R2866).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=262728;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=R2866;
RA   VanWagoner T.M., Erwin A.L., Kaul R., Mahaffey M., Zhou Y., Aggarwal G.,
RA   Chang J., Deng H., Gillett W., Haugen E., Kibukawa M., Phelps K.,
RA   Saenphimmachak C., Sivam D., Worthey E.A., Olson M.V., Stull T.L.,
RA   Smith A.L.;
RT   "Genome sequence of the invasive non-typeable isolate Haemophilus
RT   influenzae R2866.";
RL   Submitted (OCT-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   EXPRESSION IN E.COLI, FUNCTION AS AN ANTITOXIN, SUBUNIT, INDUCTION, AND
RP   OPERON STRUCTURE.
RC   STRAIN=R2866;
RX   PubMed=17496075; DOI=10.1128/jb.00290-07;
RA   Daines D.A., Wu M.H., Yuan S.Y.;
RT   "VapC-1 of nontypeable Haemophilus influenzae is a ribonuclease.";
RL   J. Bacteriol. 189:5041-5048(2007).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in E.coli neutralizes the effect of toxin VapC1. In
CC       vitro inhibits the RNase activity of VapC1.
CC       {ECO:0000269|PubMed:17496075}.
CC   -!- SUBUNIT: Forms multimers, as well forming as a complex with VapC1.
CC       {ECO:0000269|PubMed:17496075}.
CC   -!- INDUCTION: More highly expressed in early growth phase, expression
CC       decreases as cell density decreases. Part of the vapB1-vapC1 operon.
CC       {ECO:0000269|PubMed:17496075}.
CC   -!- SIMILARITY: Belongs to the VapB family. {ECO:0000305}.
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DR   EMBL; CP002277; ADO80241.1; -; Genomic_DNA.
DR   RefSeq; WP_005649046.1; NC_017451.1.
DR   AlphaFoldDB; E4QWH3; -.
DR   SMR; E4QWH3; -.
DR   GeneID; 66614919; -.
DR   KEGG; hiz:R2866_0252; -.
DR   PATRIC; fig|262728.6.peg.259; -.
DR   HOGENOM; CLU_162018_2_1_6; -.
DR   OMA; HITRVFQ; -.
DR   Proteomes; UP000002314; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR007159; SpoVT-AbrB_dom.
DR   InterPro; IPR037914; SpoVT-AbrB_sf.
DR   Pfam; PF04014; MazE_antitoxin; 1.
DR   SMART; SM00966; SpoVT_AbrB; 1.
DR   SUPFAM; SSF89447; SSF89447; 1.
DR   PROSITE; PS51740; SPOVT_ABRB; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Toxin-antitoxin system.
FT   CHAIN           1..78
FT                   /note="Antitoxin VapB1"
FT                   /id="PRO_0000408042"
FT   DOMAIN          3..44
FT                   /note="SpoVT-AbrB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01076"
SQ   SEQUENCE   78 AA;  9037 MW;  468D048ED7C5C720 CRC64;
     MLTKVFQSGN SQAVRIPMDF RFDVDTVEIF RKENGDVVLR PVSKKTDDFL ALFEGFDETF
     IQALEARDDL PPQERENL