VAPB2_HAEI8
ID VAPB2_HAEI8 Reviewed; 77 AA.
AC Q4QLV9;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Antitoxin VapB2;
DE AltName: Full=NTHi VapB2 {ECO:0000303|PubMed:27672196};
GN Name=vapB2 {ECO:0000303|PubMed:27672196}; OrderedLocusNames=NTHI1121;
OS Haemophilus influenzae (strain 86-028NP).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=281310;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=86-028NP;
RX PubMed=15968074; DOI=10.1128/jb.187.13.4627-4636.2005;
RA Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B.,
RA Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O.,
RA Munson R.S. Jr.;
RT "Genomic sequence of an otitis media isolate of nontypeable Haemophilus
RT influenzae: comparative study with H. influenzae serotype d, strain KW20.";
RL J. Bacteriol. 187:4627-4636(2005).
RN [2]
RP FUNCTION, DOMAIN, AND MUTAGENESIS OF TRP-48; PHE-51; PHE-52; ASP-61; PHE-62
RP AND ARG-66.
RC STRAIN=86-028NP;
RX PubMed=27672196; DOI=10.1128/jb.00529-16;
RA Walling L.R., Butler J.S.;
RT "Structural determinants for antitoxin identity and insulation of cross
RT talk between homologous toxin-antitoxin systems.";
RL J. Bacteriol. 198:3287-3295(2016).
CC -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system
CC (PubMed:27672196). Neutralizes the effect of cognate toxin VapC2 but
CC not non-cognate toxin VapC2 (PubMed:27672196).
CC {ECO:0000269|PubMed:27672196}.
CC -!- SUBUNIT: Probably forms a complex with cognate toxin VapC2.
CC {ECO:0000305|PubMed:27672196}.
CC -!- DOMAIN: The C-terminal 33 residues are required to neutralize cognate
CC toxin VapC2 (PubMed:27672196). {ECO:0000269|PubMed:27672196}.
CC -!- SIMILARITY: Belongs to the VapB family. {ECO:0000305}.
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DR EMBL; CP000057; AAX87988.1; -; Genomic_DNA.
DR RefSeq; WP_005648011.1; NC_007146.2.
DR AlphaFoldDB; Q4QLV9; -.
DR SMR; Q4QLV9; -.
DR EnsemblBacteria; AAX87988; AAX87988; NTHI1121.
DR GeneID; 66614087; -.
DR KEGG; hit:NTHI1121; -.
DR HOGENOM; CLU_162018_3_1_6; -.
DR OMA; VFTNNRS; -.
DR OrthoDB; 2047723at2; -.
DR Proteomes; UP000002525; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR InterPro; IPR007159; SpoVT-AbrB_dom.
DR InterPro; IPR037914; SpoVT-AbrB_sf.
DR Pfam; PF04014; MazE_antitoxin; 1.
DR SUPFAM; SSF89447; SSF89447; 1.
DR PROSITE; PS51740; SPOVT_ABRB; 1.
PE 1: Evidence at protein level;
KW Toxin-antitoxin system.
FT CHAIN 1..77
FT /note="Antitoxin VapB2"
FT /id="PRO_0000440070"
FT DOMAIN 4..46
FT /note="SpoVT-AbrB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01076"
FT MUTAGEN 48
FT /note="W->G: Loss of antitoxin activity for VapC2."
FT /evidence="ECO:0000269|PubMed:27672196"
FT MUTAGEN 51
FT /note="F->V: Partial loss of antitoxin activity for VapC2."
FT /evidence="ECO:0000269|PubMed:27672196"
FT MUTAGEN 52
FT /note="F->V: Increased antitoxin activity for VapC2."
FT /evidence="ECO:0000269|PubMed:27672196"
FT MUTAGEN 61
FT /note="D->A: Increased antitoxin activity for VapC2."
FT /evidence="ECO:0000269|PubMed:27672196"
FT MUTAGEN 62
FT /note="F->V: Protein very unstable, thus loss of antitoxin
FT activity for VapC2."
FT /evidence="ECO:0000269|PubMed:27672196"
FT MUTAGEN 66
FT /note="R->G: Wild-type antitoxin activity."
FT /evidence="ECO:0000269|PubMed:27672196"
SQ SEQUENCE 77 AA; 8881 MW; 50489BBE4678CD6D CRC64;
MIEASVFMTN RSQAVRLPAE VRFSEEIKKL SVRVSGSDRI LSPLNQSWDS FFLNDQAVSD
DFMNEREIAF QPEREAL
