ID   VAPB2_RICFE             Reviewed;          78 AA.
AC   Q4UNB3;
DT   04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Antitoxin VapB2;
GN   Name=vapB2; OrderedLocusNames=RF_0094;
OS   Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=315456;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
RN   [2]
RP   FUNCTION AS AN ANTITOXIN, AND EXPRESSION IN E.COLI OR S.CEREVISIAE.
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=22046301; DOI=10.1371/journal.pone.0026528;
RA   Audoly G., Vincentelli R., Edouard S., Georgiades K., Mediannikov O.,
RA   Gimenez G., Socolovschi C., Mege J.L., Cambillau C., Raoult D.;
RT   "Effect of rickettsial toxin VapC on its eukaryotic host.";
RL   PLoS ONE 6:E26528-E26528(2011).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH VAPC2 AND DNA,
RP   SUBUNIT, AND DNA-BINDING.
RX   PubMed=22140099; DOI=10.1093/nar/gkr1167;
RA   Mate M.J., Vincentelli R., Foos N., Raoult D., Cambillau C.,
RA   Ortiz-Lombardia M.;
RT   "Crystal structure of the DNA-bound VapBC2 antitoxin/toxin pair from
RT   Rickettsia felis.";
RL   Nucleic Acids Res. 40:3245-3258(2012).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in E.coli or S.cerevisiae neutralizes the effect of
CC       cognate toxin VapC2, partially inhibits the RNase activity of VapC2.
CC       {ECO:0000269|PubMed:22046301}.
CC   -!- SUBUNIT: Forms complexes with VapC2; probably VapC2(4):VapB2(2) in the
CC       absence of DNA, and VapC2(4):VapB2(4) in the presence of DNA.
CC       Crystallizes as heterodimers with stoichiometry VapC2(4):VapB2(4) in
CC       the presence of its probable promoter DNA. The heterodimers are in
CC       contact via alternative VapC-VapC and VapB-VapB interactions. This
CC       subunit contacts DNA. {ECO:0000269|PubMed:22140099}.
CC   -!- SIMILARITY: Belongs to the VapB family. {ECO:0000305}.
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DR   EMBL; CP000053; AAY60945.1; -; Genomic_DNA.
DR   RefSeq; WP_011270448.1; NC_007109.1.
DR   PDB; 3ZVK; X-ray; 2.50 A; E/F/G/H=1-78.
DR   PDBsum; 3ZVK; -.
DR   AlphaFoldDB; Q4UNB3; -.
DR   SMR; Q4UNB3; -.
DR   STRING; 315456.RF_0094; -.
DR   EnsemblBacteria; AAY60945; AAY60945; RF_0094.
DR   KEGG; rfe:RF_0094; -.
DR   eggNOG; COG4456; Bacteria.
DR   HOGENOM; CLU_162018_2_2_5; -.
DR   OrthoDB; 2047723at2; -.
DR   Proteomes; UP000008548; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR007159; SpoVT-AbrB_dom.
DR   InterPro; IPR037914; SpoVT-AbrB_sf.
DR   Pfam; PF04014; MazE_antitoxin; 1.
DR   SUPFAM; SSF89447; SSF89447; 1.
DR   PROSITE; PS51740; SPOVT_ABRB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Toxin-antitoxin system.
FT   CHAIN           1..78
FT                   /note="Antitoxin VapB2"
FT                   /id="PRO_0000432240"
FT   DOMAIN          4..44
FT                   /note="SpoVT-AbrB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01076"
FT   STRAND          3..9
FT                   /evidence="ECO:0007829|PDB:3ZVK"
FT   STRAND          12..17
FT                   /evidence="ECO:0007829|PDB:3ZVK"
FT   HELIX           19..21
FT                   /evidence="ECO:0007829|PDB:3ZVK"
FT   STRAND          24..33
FT                   /evidence="ECO:0007829|PDB:3ZVK"
FT   STRAND          36..41
FT                   /evidence="ECO:0007829|PDB:3ZVK"
FT   HELIX           46..57
FT                   /evidence="ECO:0007829|PDB:3ZVK"
FT   STRAND          61..64
FT                   /evidence="ECO:0007829|PDB:3ZVK"
SQ   SEQUENCE   78 AA;  9010 MW;  0735A48DA5CA5229 CRC64;
     MNKAKIFMNG QSQAVRLPKE FRFSVKEVSV IPLGKGIVLQ PLPNSWKDVF QEMAEISSDD
     IFPEGRKDLP PQKRKYFE