ID   VAPB3_MYCTU             Reviewed;          88 AA.
AC   P9WJ59; L0T6U4; O06416; Q7D9N5;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Antitoxin VapB3;
GN   Name=vapB3; OrderedLocusNames=Rv0550c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
RN   [3]
RP   EXPRESSION IN M.SMEGMATIS, FUNCTION AS AN ANTITOXIN, AND INDUCTION DURING
RP   MACROPHAGE INFECTION.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [4]
RP   INDUCTION IN PERSISTER CELLS.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21673191; DOI=10.1128/mbio.00100-11;
RA   Keren I., Minami S., Rubin E., Lewis K.;
RT   "Characterization and transcriptome analysis of Mycobacterium tuberculosis
RT   persisters.";
RL   MBio 2:E00100-E00111(2011).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in M.smegmatis neutralizes the effect of cognate toxin
CC       VapC3. {ECO:0000269|PubMed:20011113}.
CC   -!- INDUCTION: Induced during infection of mouse macrophages
CC       (PubMed:20011113). Induced in persister cells in response to D-
CC       cycloserine (PubMed:21673191). {ECO:0000269|PubMed:20011113,
CC       ECO:0000269|PubMed:21673191}.
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DR   EMBL; AL123456; CCP43288.1; -; Genomic_DNA.
DR   PIR; A70548; A70548.
DR   RefSeq; NP_215064.1; NC_000962.3.
DR   RefSeq; WP_003402915.1; NZ_NVQJ01000036.1.
DR   AlphaFoldDB; P9WJ59; -.
DR   STRING; 83332.Rv0550c; -.
DR   PaxDb; P9WJ59; -.
DR   DNASU; 887515; -.
DR   GeneID; 45424514; -.
DR   GeneID; 887515; -.
DR   KEGG; mtu:Rv0550c; -.
DR   TubercuList; Rv0550c; -.
DR   eggNOG; ENOG503363Q; Bacteria.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0045926; P:negative regulation of growth; IMP:MTBBASE.
DR   GO; GO:0075136; P:response to host; IEP:MTBBASE.
DR   InterPro; IPR009956; Post-segregation_anti-tox_CcdA.
DR   Pfam; PF07362; CcdA; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..88
FT                   /note="Antitoxin VapB3"
FT                   /id="PRO_0000408053"
SQ   SEQUENCE   88 AA;  9544 MW;  82A6144124328836 CRC64;
     MLSRRTKTIV VCTLVCMARL NVYVPDELAE RARARGLNVS ALTQAAISAE LENSATDAWL
     EGLEPRSTGA RHDDVLGAID AARDEFEA