ID   VAPB5_MYCTU             Reviewed;          86 AA.
AC   P9WF19; L0T7A0; P96916; Q7D9I5;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Putative antitoxin VapB5;
GN   Name=vapB5; OrderedLocusNames=Rv0626;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [4]
RP   SUBSTRATE SPECIFICITY, AND DOMAIN.
RC   STRAIN=H37Rv;
RX   PubMed=25622615; DOI=10.1128/jb.02508-14;
RA   Jin G., Pavelka M.S. Jr., Butler J.S.;
RT   "Structure-function analysis of VapB4 antitoxin identifies critical
RT   features of a minimal VapC4 toxin-binding module.";
RL   J. Bacteriol. 197:1197-1207(2015).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) IN COMPLEX WITH VAPC5, AND SUBUNIT.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=18952600; DOI=10.1074/jbc.m805061200;
RA   Miallau L., Faller M., Chiang J., Arbing M., Guo F., Cascio D.,
RA   Eisenberg D.;
RT   "Structure and proposed activity of a member of the VapBC family of toxin-
RT   antitoxin systems. VapBC-5 from Mycobacterium tuberculosis.";
RL   J. Biol. Chem. 284:276-283(2009).
CC   -!- FUNCTION: Probable antitoxin component of a type II toxin-antitoxin
CC       (TA) system. The cognate toxin is VapC5. {ECO:0000269|PubMed:18952600}.
CC   -!- SUBUNIT: Forms a complex with VapC5. {ECO:0000269|PubMed:18952600}.
CC   -!- DOMAIN: The C-terminal domain (residues 55-86) does not neutralize non-
CC       cognate antitoxin VapC4; exchanging the N-terminus (residues 1-54) with
CC       VapB4 however allows neutralization of VapC4, while exchanging the C-
CC       terminal domain (residues 55-86) does not.
CC       {ECO:0000269|PubMed:25622615}.
CC   -!- SIMILARITY: Belongs to the phD/YefM antitoxin family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43367.1; -; Genomic_DNA.
DR   PIR; G70611; G70611.
DR   RefSeq; NP_215140.1; NC_000962.3.
DR   RefSeq; WP_003403244.1; NZ_NVQJ01000033.1.
DR   PDB; 3DBO; X-ray; 1.76 A; A=1-86.
DR   PDBsum; 3DBO; -.
DR   AlphaFoldDB; P9WF19; -.
DR   SMR; P9WF19; -.
DR   STRING; 83332.Rv0626; -.
DR   PaxDb; P9WF19; -.
DR   DNASU; 887996; -.
DR   GeneID; 887996; -.
DR   KEGG; mtu:Rv0626; -.
DR   TubercuList; Rv0626; -.
DR   eggNOG; COG4118; Bacteria.
DR   OMA; HFVRHIS; -.
DR   PhylomeDB; P9WF19; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0015643; F:toxic substance binding; IDA:MTBBASE.
DR   GO; GO:0097351; F:toxin sequestering activity; IBA:GO_Central.
DR   GO; GO:0045927; P:positive regulation of growth; IDA:MTBBASE.
DR   DisProt; DP00889; -.
DR   InterPro; IPR006442; Antitoxin_Phd/YefM.
DR   InterPro; IPR036165; YefM-like_sf.
DR   Pfam; PF02604; PhdYeFM_antitox; 1.
DR   SUPFAM; SSF143120; SSF143120; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..86
FT                   /note="Putative antitoxin VapB5"
FT                   /id="PRO_0000408046"
FT   HELIX           51..58
FT                   /evidence="ECO:0007829|PDB:3DBO"
FT   HELIX           67..75
FT                   /evidence="ECO:0007829|PDB:3DBO"
SQ   SEQUENCE   86 AA;  9493 MW;  6B0E7DD0BB8BE563 CRC64;
     MSEVASRELR NDTAGVLRRV RAGEDVTITV SGRPVAVLTP VRPRRRRWLS KTEFLSRLRG
     AQADPGLRND LAVLAGDTTE DLGPIR