VAPB_MYCS2
ID VAPB_MYCS2 Reviewed; 84 AA.
AC A0QRY5;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Antitoxin VapB;
GN Name=vapB; OrderedLocusNames=MSMEG_1283, MSMEI_1245;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION AS A ANTITOXIN, FUNCTION IN TRANSCRIPTION REGULATION, SUBUNIT,
RP INDUCTION, DNA-BINDING, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=19445953; DOI=10.1016/j.jmb.2009.05.006;
RA Robson J., McKenzie J.L., Cursons R., Cook G.M., Arcus V.L.;
RT "The vapBC operon from Mycobacterium smegmatis is an autoregulated toxin-
RT antitoxin module that controls growth via inhibition of translation.";
RL J. Mol. Biol. 390:353-367(2009).
RN [5]
RP FUNCTION AS AN ANTITOXIN, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=22366418; DOI=10.1128/jb.06790-11;
RA McKenzie J.L., Robson J., Berney M., Smith T.C., Ruthe A., Gardner P.P.,
RA Arcus V.L., Cook G.M.;
RT "A VapBC toxin-antitoxin module is a posttranscriptional regulator of
RT metabolic flux in mycobacteria.";
RL J. Bacteriol. 194:2189-2204(2012).
RN [6]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=22199354; DOI=10.1074/jbc.m111.286856;
RA Frampton R., Aggio R.B., Villas-Boas S.G., Arcus V.L., Cook G.M.;
RT "Toxin-antitoxin systems of Mycobacterium smegmatis are essential for cell
RT survival.";
RL J. Biol. Chem. 287:5340-5356(2012).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=24417293; DOI=10.1111/1574-6968.12380;
RA Demidenok O.I., Kaprelyants A.S., Goncharenko A.V.;
RT "Toxin-antitoxin vapBC locus participates in formation of the dormant state
RT in Mycobacterium smegmatis.";
RL FEMS Microbiol. Lett. 352:69-77(2014).
CC -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC Upon overexpression neutralizes the effect of overexpressed cognate
CC toxin VapC. Overexpression alone prevents cells from entering a
CC nonculturable dormant state, probably as it binds endogenous VapC
CC (PubMed:24417293). The TA system acts as a post-transcriptional
CC regulator of carbon metabolism; in M.smegmatis 3 TA systems (VapB-VapC,
CC MazE-MazF and Phd-Doc) may be involved in monitoring the nutritional
CC supply and physiological state of the cell, linking catabolic with
CC anabolic reactions. Translation of vapC mRNA requires VapB.
CC {ECO:0000269|PubMed:19445953, ECO:0000269|PubMed:22366418,
CC ECO:0000269|PubMed:24417293}.
CC -!- SUBUNIT: Forms a complex with cognate toxin VapC.
CC {ECO:0000269|PubMed:19445953, ECO:0000269|PubMed:22366418}.
CC -!- INDUCTION: Expression is low but constitutive, and repressed by VapB-
CC VapC. Member of the vapB-vapC operon. {ECO:0000269|PubMed:19445953}.
CC -!- DISRUPTION PHENOTYPE: The vapB-vapC operon is not essential; cells grow
CC faster than wild-type on rich and minimal glycerol-containing medium.
CC The operon deletion mutants die faster than wild-type under potassium-
CC limiting conditions, which is prevented by overexpression of vapB
CC (PubMed:24417293). The vapB antitoxin gene can be disrupted without
CC causing death, however despite elevated vapC transcription, no VapC
CC protein could be detected, suggesting that RNA processing and
CC translational coupling are important in VapC expression. A triple TA
CC mutant (missing vapB-vapC, mazE-mazF and phd-doc TA systems) survives
CC antibiotic challenge, suggesting the TA systems are not required to
CC generate drug-resistant cells. However the triple mutant is more
CC sensitive to oxidative and heat stress, and does not survive long term
CC starvation during aerobic growth on complex medium. There is a
CC difference in the level of branched-chain amino acids, which may play a
CC role in monitoring the nutritional supply and physiological state of
CC the cell. {ECO:0000269|PubMed:19445953, ECO:0000269|PubMed:22199354,
CC ECO:0000269|PubMed:22366418, ECO:0000269|PubMed:24417293}.
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DR EMBL; CP000480; ABK71133.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP37721.1; -; Genomic_DNA.
DR RefSeq; WP_003892665.1; NZ_SIJM01000042.1.
DR RefSeq; YP_885673.1; NC_008596.1.
DR AlphaFoldDB; A0QRY5; -.
DR SMR; A0QRY5; -.
DR STRING; 246196.MSMEI_1245; -.
DR EnsemblBacteria; ABK71133; ABK71133; MSMEG_1283.
DR EnsemblBacteria; AFP37721; AFP37721; MSMEI_1245.
DR GeneID; 66732745; -.
DR KEGG; msg:MSMEI_1245; -.
DR KEGG; msm:MSMEG_1283; -.
DR PATRIC; fig|246196.19.peg.1272; -.
DR eggNOG; COG4423; Bacteria.
DR OMA; IIGYDEH; -.
DR OrthoDB; 2005573at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR InterPro; IPR011660; VapB-like.
DR Pfam; PF07704; PSK_trans_fac; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Repressor; Toxin-antitoxin system;
KW Transcription; Transcription regulation.
FT CHAIN 1..84
FT /note="Antitoxin VapB"
FT /id="PRO_0000420849"
SQ SEQUENCE 84 AA; 9280 MW; 7DCCB9774047B15E CRC64;
MALSIKHPEA DRLARELAAR TGETLTEAVV MALRERLART VGRTQVVPLR EELAAIRRRC
AALPVLDDRT AESILGYDDR GLPS