VAPB_RAT
ID VAPB_RAT Reviewed; 243 AA.
AC Q9Z269;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Vesicle-associated membrane protein-associated protein B;
DE Short=VAMP-B;
DE Short=VAMP-associated protein B;
DE Short=VAP-B;
GN Name=Vapb;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Heart;
RX PubMed=9920726; DOI=10.1006/bbrc.1998.9876;
RA Nishimura Y., Hayashi M., Inada H., Tanaka T.;
RT "Molecular cloning and characterization of mammalian homologues of vesicle-
RT associated membrane protein-associated (VAMP-associated) proteins.";
RL Biochem. Biophys. Res. Commun. 254:21-26(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 176-182, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (JUL-2007) to UniProtKB.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-159, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Participates in the endoplasmic reticulum unfolded protein
CC response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular
CC calcium homeostasis regulation. {ECO:0000250|UniProtKB:O95292}.
CC -!- SUBUNIT: Homodimer, and heterodimer with VAPA. Interacts with RMDN3,
CC VAMP1 and VAMP2. Interacts (via MSP domain) with ZFYVE27. Interacts
CC with KIF5A in a ZFYVE27-dependent manner. Interacts with STARD3 (via
CC FFAT motif) (By similarity). Interacts with STARD3NL (via FFAT motif)
CC (By similarity). Interacts with CERT1 (By similarity). Interacts with
CC PLEKHA3 and SACM1L to form a ternary complex (By similarity). Interacts
CC with VPS13A (via FFAT motif) (By similarity).
CC {ECO:0000250|UniProtKB:O95292}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:O95292}; Single-pass type IV membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the VAMP-associated protein (VAP) (TC 9.B.17)
CC family. {ECO:0000305}.
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DR EMBL; AF086631; AAD13580.1; -; mRNA.
DR EMBL; BC065576; AAH65576.1; -; mRNA.
DR RefSeq; NP_068619.1; NM_021847.3.
DR AlphaFoldDB; Q9Z269; -.
DR SMR; Q9Z269; -.
DR BioGRID; 248831; 1.
DR IntAct; Q9Z269; 2.
DR MINT; Q9Z269; -.
DR STRING; 10116.ENSRNOP00000007554; -.
DR iPTMnet; Q9Z269; -.
DR PhosphoSitePlus; Q9Z269; -.
DR SwissPalm; Q9Z269; -.
DR jPOST; Q9Z269; -.
DR PaxDb; Q9Z269; -.
DR PRIDE; Q9Z269; -.
DR ABCD; Q9Z269; 1 sequenced antibody.
DR Ensembl; ENSRNOT00000007554; ENSRNOP00000007554; ENSRNOG00000005331.
DR GeneID; 60431; -.
DR KEGG; rno:60431; -.
DR UCSC; RGD:68326; rat.
DR CTD; 9217; -.
DR RGD; 68326; Vapb.
DR eggNOG; KOG0439; Eukaryota.
DR GeneTree; ENSGT00940000155769; -.
DR HOGENOM; CLU_032848_0_1_1; -.
DR InParanoid; Q9Z269; -.
DR OMA; TQRMAFK; -.
DR OrthoDB; 1332028at2759; -.
DR PhylomeDB; Q9Z269; -.
DR TreeFam; TF317024; -.
DR Reactome; R-RNO-1660661; Sphingolipid de novo biosynthesis.
DR Reactome; R-RNO-8980692; RHOA GTPase cycle.
DR Reactome; R-RNO-9013106; RHOC GTPase cycle.
DR Reactome; R-RNO-9013404; RAC2 GTPase cycle.
DR Reactome; R-RNO-9013405; RHOD GTPase cycle.
DR Reactome; R-RNO-9013408; RHOG GTPase cycle.
DR PRO; PR:Q9Z269; -.
DR Proteomes; UP000002494; Chromosome 3.
DR Bgee; ENSRNOG00000005331; Expressed in skeletal muscle tissue and 20 other tissues.
DR Genevisible; Q9Z269; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISO:RGD.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0048487; F:beta-tubulin binding; ISS:UniProtKB.
DR GO; GO:0019899; F:enzyme binding; ISS:UniProtKB.
DR GO; GO:0033149; F:FFAT motif binding; ISO:RGD.
DR GO; GO:0008017; F:microtubule binding; ISO:RGD.
DR GO; GO:0046982; F:protein heterodimerization activity; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; ISS:UniProtKB.
DR GO; GO:0090114; P:COPII-coated vesicle budding; ISO:RGD.
DR GO; GO:0090158; P:endoplasmic reticulum membrane organization; IBA:GO_Central.
DR GO; GO:0007029; P:endoplasmic reticulum organization; ISO:RGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; ISO:RGD.
DR GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; ISS:UniProtKB.
DR GO; GO:0061817; P:endoplasmic reticulum-plasma membrane tethering; IBA:GO_Central.
DR GO; GO:0036498; P:IRE1-mediated unfolded protein response; ISS:UniProtKB.
DR GO; GO:0045070; P:positive regulation of viral genome replication; ISS:UniProtKB.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR000535; MSP_dom.
DR InterPro; IPR008962; PapD-like_sf.
DR InterPro; IPR016763; VAP.
DR PANTHER; PTHR10809; PTHR10809; 2.
DR Pfam; PF00635; Motile_Sperm; 1.
DR PIRSF; PIRSF019693; VAMP-associated; 1.
DR SUPFAM; SSF49354; SSF49354; 1.
DR PROSITE; PS50202; MSP; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Direct protein sequencing; Endoplasmic reticulum;
KW Isopeptide bond; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Ubl conjugation;
KW Unfolded protein response.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:O95292"
FT CHAIN 2..243
FT /note="Vesicle-associated membrane protein-associated
FT protein B"
FT /id="PRO_0000213475"
FT TOPO_DOM 2..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT DOMAIN 7..124
FT /note="MSP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00132"
FT COILED 161..196
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:O95292"
FT MOD_RES 146
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O95292"
FT MOD_RES 159
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 206
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O95292"
FT CROSSLNK 147
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO1)"
FT /evidence="ECO:0000250|UniProtKB:O95292"
SQ SEQUENCE 243 AA; 26916 MW; 870B177B0798B4EA CRC64;
MAKVEQVLSL EPQHELKFRG PFTDVVTTNL KLGNPTDRNV CFKVKTTAPR RYCVRPNSGV
IDAGASLNVS VMLQPFDYDP NEKSKHKFMV QSMFAPPDTS DMEAVWKEAK PEDLMDSKLR
CVFELPAENA KPHDVEINKI MPTSASKTEA PVAAKPLTSP LDDAEVKKVM EECRRLQGEV
QRLREESRQL KEEDGLRARK ALPSNSPMAA LAASGKEEGL SARLLALVVL FFIVGVIIGK
IAL
