ID   VAPC1_MYCTU             Reviewed;         133 AA.
AC   P9WFC1; L0T478; O53610; Q7DAI7;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=Ribonuclease VapC1 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC1 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC1 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC1 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=Rv0065;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
RN   [3]
RP   FUNCTION, ACTIVITY REGULATION, AND COFACTOR.
RX   PubMed=22539524; DOI=10.1261/rna.031229.111;
RA   McKenzie J.L., Duyvestyn J.M., Smith T., Bendak K., Mackay J., Cursons R.,
RA   Cook G.M., Arcus V.L.;
RT   "Determination of ribonuclease sequence-specificity using Pentaprobes and
RT   mass spectrometry.";
RL   RNA 18:1267-1278(2012).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. The
CC       cognate antitoxin is VapB1 (By similarity). Has ribonuclease activity.
CC       {ECO:0000250, ECO:0000269|PubMed:22539524}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265,
CC         ECO:0000269|PubMed:22539524};
CC   -!- ACTIVITY REGULATION: Inhibited by EDTA. {ECO:0000269|PubMed:22539524}.
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AL123456; CCP42788.1; -; Genomic_DNA.
DR   PIR; B70848; B70848.
DR   RefSeq; NP_214579.1; NC_000962.3.
DR   RefSeq; WP_003400580.1; NZ_NVQJ01000005.1.
DR   AlphaFoldDB; P9WFC1; -.
DR   SMR; P9WFC1; -.
DR   STRING; 83332.Rv0065; -.
DR   PaxDb; P9WFC1; -.
DR   GeneID; 886993; -.
DR   KEGG; mtu:Rv0065; -.
DR   TubercuList; Rv0065; -.
DR   eggNOG; COG4113; Bacteria.
DR   OMA; YPVEPLM; -.
DR   PhylomeDB; P9WFC1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   CDD; cd09873; PIN_Pae0151-like; 1.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR044153; PIN_Pae0151-like.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..133
FT                   /note="Ribonuclease VapC1"
FT                   /id="PRO_0000407864"
FT   BINDING         7
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         98
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   133 AA;  14372 MW;  965C3052370F54F4 CRC64;
     MDECVVDAAA VVDALAGKGA SAIVLRGLLK ESISNAPHLL DAEVGHALRR AVLSDEISEE
     QARAALDALP YLIDNRYPHS PRLIEYTWQL RHNVTFYDAL YVALATALDV PLLTGDSRLA
     AAPGLPCEIK LVR