ID   VAPC3_MYCTO             Reviewed;         137 AA.
AC   P9WFB6; L0T708; O06415; Q7D9N6;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Ribonuclease VapC3 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC3 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC3 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC3 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=MT0574;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its toxic effect is neutralized by coexpression with cognate
CC       antitoxin VapB3 (By similarity). {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AE000516; AAK44797.1; -; Genomic_DNA.
DR   PIR; H70547; H70547.
DR   RefSeq; WP_003898500.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WFB6; -.
DR   SMR; P9WFB6; -.
DR   EnsemblBacteria; AAK44797; AAK44797; MT0574.
DR   GeneID; 45424513; -.
DR   KEGG; mtc:MT0574; -.
DR   PATRIC; fig|83331.31.peg.605; -.
DR   HOGENOM; CLU_121774_0_1_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   CDD; cd09873; PIN_Pae0151-like; 1.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR044153; PIN_Pae0151-like.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..137
FT                   /note="Ribonuclease VapC3"
FT                   /id="PRO_0000428567"
FT   DOMAIN          12..129
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         15
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         105
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   137 AA;  14700 MW;  16A91120CA498A7E CRC64;
     MRASPTSPPE QVVVDASAMV DLLARTSDRC SAVRARLART AMHAPAHFDA EVLSALGRMQ
     RAGALTVAYV DAALEELRQV PVTRHGLSSL LAGAWSRRDT LRLTDALYVE LAETAGLVLL
     TTDERLARAW PSAHAIG