VAPC3_PYRAE
ID VAPC3_PYRAE Reviewed; 131 AA.
AC Q8ZZP3;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Ribonuclease VapC3 {ECO:0000255|HAMAP-Rule:MF_00265};
DE Short=RNase VapC3 {ECO:0000255|HAMAP-Rule:MF_00265};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE AltName: Full=Putative toxin VapC3 {ECO:0000255|HAMAP-Rule:MF_00265};
GN Name=vapC3 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=PAE0151;
OS Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP
OS 104966 / NBRC 100827 / IM2).
OC Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC Pyrobaculum.
OX NCBI_TaxID=178306;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2;
RX PubMed=11792869; DOI=10.1073/pnas.241636498;
RA Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I.,
RA Miller J.H.;
RT "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum
RT aerophilum.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).
RN [2]
RP POSSIBLE FUNCTION.
RC STRAIN=ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2;
RX PubMed=15718296; DOI=10.1093/nar/gki201;
RA Pandey D.P., Gerdes K.;
RT "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT host-associated prokaryotes.";
RL Nucleic Acids Res. 33:966-976(2005).
RN [3]
RP FUNCTION, ACTIVITY REGULATION, AND COFACTOR.
RX PubMed=22539524; DOI=10.1261/rna.031229.111;
RA McKenzie J.L., Duyvestyn J.M., Smith T., Bendak K., Mackay J., Cursons R.,
RA Cook G.M., Arcus V.L.;
RT "Determination of ribonuclease sequence-specificity using Pentaprobes and
RT mass spectrometry.";
RL RNA 18:1267-1278(2012).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 3-131 IN COMPLEX WITH MANGANESE,
RP COFACTOR, AND SUBUNIT.
RC STRAIN=ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2;
RX PubMed=18398909; DOI=10.1002/prot.22048;
RA Bunker R.D., McKenzie J.L., Baker E.N., Arcus V.L.;
RT "Crystal structure of PAE0151 from Pyrobaculum aerophilum, a PIN-domain
RT (VapC) protein from a toxin-antitoxin operon.";
RL Proteins 72:510-518(2008).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. Has
CC ribonuclease activity. {ECO:0000269|PubMed:22539524}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00265,
CC ECO:0000269|PubMed:18398909, ECO:0000269|PubMed:22539524};
CC -!- ACTIVITY REGULATION: Inhibited by EDTA. {ECO:0000269|PubMed:22539524}.
CC -!- SUBUNIT: Homodimer. Forms a complex with putative antitoxin VapB3,
CC possibly VapB(2)-VapC(2). {ECO:0000269|PubMed:18398909}.
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR EMBL; AE009441; AAL62596.1; -; Genomic_DNA.
DR PDB; 2FE1; X-ray; 2.20 A; A=1-131.
DR PDBsum; 2FE1; -.
DR AlphaFoldDB; Q8ZZP3; -.
DR SMR; Q8ZZP3; -.
DR STRING; 178306.PAE0151; -.
DR EnsemblBacteria; AAL62596; AAL62596; PAE0151.
DR KEGG; pai:PAE0151; -.
DR PATRIC; fig|178306.9.peg.108; -.
DR eggNOG; arCOG00726; Archaea.
DR HOGENOM; CLU_121774_1_4_2; -.
DR InParanoid; Q8ZZP3; -.
DR OMA; SNAIWKH; -.
DR EvolutionaryTrace; Q8ZZP3; -.
DR Proteomes; UP000002439; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR CDD; cd09873; PIN_Pae0151-like; 1.
DR HAMAP; MF_00265; VapC_Nob1; 1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR InterPro; IPR044153; PIN_Pae0151-like.
DR InterPro; IPR022907; VapC_family.
DR Pfam; PF01850; PIN; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Reference proteome; Toxin-antitoxin system.
FT CHAIN 1..131
FT /note="Ribonuclease VapC3"
FT /id="PRO_0000407903"
FT DOMAIN 4..121
FT /note="PINc"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 6
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 100
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 118
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT STRAND 2..5
FT /evidence="ECO:0007829|PDB:2FE1"
FT HELIX 7..11
FT /evidence="ECO:0007829|PDB:2FE1"
FT STRAND 14..16
FT /evidence="ECO:0007829|PDB:2FE1"
FT HELIX 21..29
FT /evidence="ECO:0007829|PDB:2FE1"
FT STRAND 31..36
FT /evidence="ECO:0007829|PDB:2FE1"
FT HELIX 37..52
FT /evidence="ECO:0007829|PDB:2FE1"
FT HELIX 58..72
FT /evidence="ECO:0007829|PDB:2FE1"
FT STRAND 75..78
FT /evidence="ECO:0007829|PDB:2FE1"
FT HELIX 80..82
FT /evidence="ECO:0007829|PDB:2FE1"
FT HELIX 84..94
FT /evidence="ECO:0007829|PDB:2FE1"
FT HELIX 98..110
FT /evidence="ECO:0007829|PDB:2FE1"
FT STRAND 113..115
FT /evidence="ECO:0007829|PDB:2FE1"
FT HELIX 119..124
FT /evidence="ECO:0007829|PDB:2FE1"
FT TURN 126..128
FT /evidence="ECO:0007829|PDB:2FE1"
SQ SEQUENCE 131 AA; 14625 MW; 3AE51CCED30B02EA CRC64;
MKLVVDASAI AALYVPEERS EQAERAVSQA QELHTLDLAA YEVANDLWKH ARRGLLREDE
ASNMLEELWE FFKALKVHSY AEVLKDAFAL ALKHGVTVYD AAYVALAEKI GGKLLTLDRQ
LAEKFPALVT P
