ID   VAPC4_METJA             Reviewed;         131 AA.
AC   Q58716;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Ribonuclease VapC4 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC4 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Putative toxin VapC4 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC4 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=MJ1320;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its cognate antitoxin is VapB4 (By similarity).
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; L77117; AAB99330.1; -; Genomic_DNA.
DR   PIR; G64464; G64464.
DR   RefSeq; WP_010870837.1; NC_000909.1.
DR   AlphaFoldDB; Q58716; -.
DR   SMR; Q58716; -.
DR   STRING; 243232.MJ_1320; -.
DR   EnsemblBacteria; AAB99330; AAB99330; MJ_1320.
DR   GeneID; 1452222; -.
DR   KEGG; mja:MJ_1320; -.
DR   eggNOG; arCOG04312; Archaea.
DR   HOGENOM; CLU_107892_1_0_2; -.
DR   InParanoid; Q58716; -.
DR   OMA; DEMIINY; -.
DR   OrthoDB; 118267at2157; -.
DR   PhylomeDB; Q58716; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR041120; PIN_9.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF18477; PIN_9; 1.
DR   SMART; SM00670; PINc; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..131
FT                   /note="Ribonuclease VapC4"
FT                   /id="PRO_0000107274"
FT   DOMAIN          4..106
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         7
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         102
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   131 AA;  15452 MW;  BD4589FBD5A56693 CRC64;
     MYKIVPDTNF LIYVFKHKIN FDYEIERALN TKFEIVILSP IKEELERLLK SRDLKGKEKL
     AVNLALAKIK NYKLVDYTAN YADEAILNYA KENENVIVAT NDKELKEKLM ENNIPVMVVR
     QKKYFEIFGM V