ID   VAPC8_MYCTO             Reviewed;         112 AA.
AC   P9WFB0; L0T768; O06774; Q7D9G5;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Ribonuclease VapC8;
DE            Short=RNase VapC8;
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin VapC8;
GN   Name=vapC8; OrderedLocusNames=MT0693;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. The cognate antitoxin is VapB8 (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK44919.1; -; Genomic_DNA.
DR   PIR; D70535; D70535.
DR   RefSeq; WP_003403405.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WFB0; -.
DR   SMR; P9WFB0; -.
DR   EnsemblBacteria; AAK44919; AAK44919; MT0693.
DR   KEGG; mtc:MT0693; -.
DR   PATRIC; fig|83331.31.peg.737; -.
DR   HOGENOM; CLU_118482_6_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..112
FT                   /note="Ribonuclease VapC8"
FT                   /id="PRO_0000428570"
FT   DOMAIN          10..109
FT                   /note="PINc"
FT   BINDING         12
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
FT   BINDING         101
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   112 AA;  11590 MW;  ED398523D598FDF9 CRC64;
     MTEGEVGVGL LDTSVFIARE SGGAIADLPE RVALSVMTIG ELQLGLLNAG DSATRSRRAD
     TLALARTADQ IPVSEAVMIS LARLVADCRA AGVRRSVKLT DALIAATAEI KV