ID   VAPC8_MYCTU             Reviewed;         112 AA.
AC   P9WFB1; L0T768; O06774; Q7D9G5;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=Ribonuclease VapC8;
DE            Short=RNase VapC8;
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin VapC8;
GN   Name=vapC8; OrderedLocusNames=Rv0665;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. The cognate antitoxin is VapB8 (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43408.1; -; Genomic_DNA.
DR   PIR; D70535; D70535.
DR   RefSeq; NP_215179.1; NC_000962.3.
DR   RefSeq; WP_003403405.1; NZ_NVQJ01000007.1.
DR   AlphaFoldDB; P9WFB1; -.
DR   SMR; P9WFB1; -.
DR   STRING; 83332.Rv0665; -.
DR   PaxDb; P9WFB1; -.
DR   DNASU; 888149; -.
DR   GeneID; 888149; -.
DR   KEGG; mtu:Rv0665; -.
DR   TubercuList; Rv0665; -.
DR   eggNOG; COG1487; Bacteria.
DR   OMA; IACACPA; -.
DR   PhylomeDB; P9WFB1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..112
FT                   /note="Ribonuclease VapC8"
FT                   /id="PRO_0000407871"
FT   DOMAIN          10..109
FT                   /note="PINc"
FT   BINDING         12
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
FT   BINDING         101
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   112 AA;  11590 MW;  ED398523D598FDF9 CRC64;
     MTEGEVGVGL LDTSVFIARE SGGAIADLPE RVALSVMTIG ELQLGLLNAG DSATRSRRAD
     TLALARTADQ IPVSEAVMIS LARLVADCRA AGVRRSVKLT DALIAATAEI KV