VAPC9_ARCFU
ID VAPC9_ARCFU Reviewed; 135 AA.
AC O29664;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Ribonuclease VapC9 {ECO:0000255|HAMAP-Rule:MF_00265};
DE Short=RNase VapC9 {ECO:0000255|HAMAP-Rule:MF_00265};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE AltName: Full=Putative toxin VapC9 {ECO:0000255|HAMAP-Rule:MF_00265};
GN Name=vapC9 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=AF_0591;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
RN [2]
RP POSSIBLE FUNCTION.
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=15718296; DOI=10.1093/nar/gki201;
RA Pandey D.P., Gerdes K.;
RT "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT host-associated prokaryotes.";
RL Nucleic Acids Res. 33:966-976(2005).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS), AND SUBUNIT.
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=15211526; DOI=10.1002/prot.20090;
RA Levin I., Schwarzenbacher R., Page R., Abdubek P., Ambing E., Biorac T.,
RA Brinen L.S., Campbell J., Canaves J.M., Chiu H.J., Dai X., Deacon A.M.,
RA DiDonato M., Elsliger M.A., Floyd R., Godzik A., Grittini C.,
RA Grzechnik S.K., Hampton E., Jaroszewski L., Karlak C., Klock H.E.,
RA Koesema E., Kovarik J.S., Kreusch A., Kuhn P., Lesley S.A., McMullan D.,
RA McPhillips T.M., Miller M.D., Morse A., Moy K., Ouyang J., Quijano K.,
RA Reyes R., Rezezadeh F., Robb A., Sims E., Spraggon G., Stevens R.C.,
RA van den Bedem H., Velasquez J., Vincent J., von Delft F., Wang X., West B.,
RA Wolf G., Xu Q., Hodgson K.O., Wooley J., Wilson I.A.;
RT "Crystal structure of a PIN (PilT N-terminus) domain (AF0591) from
RT Archaeoglobus fulgidus at 1.90 A resolution.";
RL Proteins 56:404-408(2004).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC RNase. {ECO:0000255|HAMAP-Rule:MF_00265}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC -!- SUBUNIT: Dimer. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR EMBL; AE000782; AAB90656.1; -; Genomic_DNA.
DR PIR; G69323; G69323.
DR RefSeq; WP_010878095.1; NC_000917.1.
DR PDB; 1O4W; X-ray; 1.90 A; A=1-135.
DR PDBsum; 1O4W; -.
DR AlphaFoldDB; O29664; -.
DR SMR; O29664; -.
DR STRING; 224325.AF_0591; -.
DR EnsemblBacteria; AAB90656; AAB90656; AF_0591.
DR GeneID; 24794191; -.
DR KEGG; afu:AF_0591; -.
DR eggNOG; arCOG04312; Archaea.
DR HOGENOM; CLU_107892_1_0_2; -.
DR OMA; LMMPVEL; -.
DR OrthoDB; 112878at2157; -.
DR PhylomeDB; O29664; -.
DR EvolutionaryTrace; O29664; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR HAMAP; MF_00265; VapC_Nob1; 1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR041120; PIN_9.
DR InterPro; IPR002716; PIN_dom.
DR InterPro; IPR022907; VapC_family.
DR Pfam; PF18477; PIN_9; 1.
DR SMART; SM00670; PINc; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Reference proteome; Toxin-antitoxin system.
FT CHAIN 1..135
FT /note="Ribonuclease VapC9"
FT /id="PRO_0000407902"
FT DOMAIN 15..118
FT /note="PINc"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 17
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 88
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT STRAND 12..16
FT /evidence="ECO:0007829|PDB:1O4W"
FT HELIX 18..27
FT /evidence="ECO:0007829|PDB:1O4W"
FT HELIX 31..37
FT /evidence="ECO:0007829|PDB:1O4W"
FT STRAND 40..46
FT /evidence="ECO:0007829|PDB:1O4W"
FT HELIX 47..59
FT /evidence="ECO:0007829|PDB:1O4W"
FT HELIX 62..75
FT /evidence="ECO:0007829|PDB:1O4W"
FT STRAND 78..81
FT /evidence="ECO:0007829|PDB:1O4W"
FT HELIX 88..98
FT /evidence="ECO:0007829|PDB:1O4W"
FT STRAND 101..103
FT /evidence="ECO:0007829|PDB:1O4W"
FT HELIX 107..115
FT /evidence="ECO:0007829|PDB:1O4W"
SQ SEQUENCE 135 AA; 15467 MW; C784D6F4A8183854 CRC64;
MEADRGRNNK VRCAVVDTNV LMYVYLNKAD VVGQLREFGF SRFLITASVK RELEKLEMSL
RGKEKVAARF ALKLLEHFEV VETESEGDPS LIEAAEKYGC ILITNDKELK RKAKQRGIPV
GYLKEDKRVF VELLD
