ID   VAPC9_MYCTO             Reviewed;         127 AA.
AC   P9WFA8; L0T6X4; P64773; P71550;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Ribonuclease VapC9 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC9 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC9 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC9 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=MT0988;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. The cognate antitoxin is VapB9 (By similarity).
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AE000516; AAK45236.1; -; Genomic_DNA.
DR   PIR; F70717; F70717.
DR   RefSeq; WP_003404903.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WFA8; -.
DR   SMR; P9WFA8; -.
DR   EnsemblBacteria; AAK45236; AAK45236; MT0988.
DR   KEGG; mtc:MT0988; -.
DR   PATRIC; fig|83331.31.peg.1060; -.
DR   HOGENOM; CLU_121774_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   CDD; cd09873; PIN_Pae0151-like; 1.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR044153; PIN_Pae0151-like.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..127
FT                   /note="Ribonuclease VapC9"
FT                   /id="PRO_0000428571"
FT   DOMAIN          2..115
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         5
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         91
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   127 AA;  13859 MW;  6804DC5EE9269AC0 CRC64;
     MIVVDASAAL AALLNDGQAR QLIAAERLHV PHLVDSEIAS GLRRLAQRDR LGAADGRRAL
     QTWRRLAVTR YPVVGLFERI WEIRANLSAY DASYVALAEA LNCALVTADL RLSDTGQAQC
     PITVVPR