ID   VAP_CERV                Reviewed;         128 AA.
AC   P05398;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   29-SEP-2021, entry version 62.
DE   RecName: Full=Virion-associated protein;
DE            Short=Vap;
DE   AltName: Full=Protein 3;
DE            Short=P3;
GN   ORFNames=ORF III;
OS   Carnation etched ring virus (CERV).
OC   Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC   Ortervirales; Caulimoviridae; Caulimovirus.
OX   NCBI_TaxID=10640;
OH   NCBI_TaxID=3570; Dianthus caryophyllus (Carnation) (Clove pink).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16453731; DOI=10.1002/j.1460-2075.1986.tb04614.x;
RA   Hull R., Sadler J., Longstaff M.;
RT   "The sequence of carnation etched ring virus DNA: comparison with
RT   cauliflower mosaic virus and retroviruses.";
RL   EMBO J. 5:3083-3090(1986).
CC   -!- FUNCTION: Plays a role in virus cell-to-cell and plant-to-plant
CC       transmission. Interacts with virion icosahedral capsid and movement
CC       protein, thereby facilitating virion cell-to-cell transmission through
CC       plasmodesmata opened by viral movement protein. Also interacts with
CC       aphid transmission factor, attaching the virion to aphid stylet when
CC       the animal feeds on an virus infected plant. Aphid saliva may later
CC       detach the virion, inducing release of infectious particles when the
CC       animal feeds on a new plant (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homotetramer, through coiled-coil domain. Homotrimer when
CC       interacts with icosehadral capsid. Interacts with capsid protein, and
CC       with Movement protein (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host cell junction, host
CC       plasmodesma.
CC   -!- SIMILARITY: Belongs to the caulimovirus ORF III family. {ECO:0000305}.
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DR   EMBL; X04658; CAA28358.1; -; Genomic_DNA.
DR   PIR; S00852; S00852.
DR   RefSeq; NP_612575.1; NC_003498.1.
DR   SMR; P05398; -.
DR   GeneID; 935424; -.
DR   KEGG; vg:935424; -.
DR   Proteomes; UP000008446; Genome.
DR   GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   InterPro; IPR004986; Caulimo_virion-assoc.
DR   Pfam; PF03310; Cauli_DNA-bind; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Disulfide bond; Host cell junction; Reference proteome;
KW   Virion.
FT   CHAIN           1..128
FT                   /note="Virion-associated protein"
FT                   /id="PRO_0000222081"
FT   REGION          98..128
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          122..128
FT                   /note="Capsid binding"
FT                   /evidence="ECO:0000250"
FT   COILED          1..30
FT                   /evidence="ECO:0000250"
FT   COILED          37..58
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        98..116
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        59
FT                   /note="Interchain (with C-61 in multimeric partner 1)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        61
FT                   /note="Interchain (with C-59 in multimeric partner 2)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   128 AA;  14232 MW;  FABA7BFDDE378DBB CRC64;
     MNLATIASEI EVVKTNQKTI ESKIDQILAK IGSTPDESSN LESVAAKIIS DLTKEMKECH
     CNKEIVEILN KDKAIIPSPE QDSIQKRLSE PQYTFPNFDV GNEGMGSSTN PNALKWPPTE
     KPQPWPPR