ID   VARIA_DERVA             Reviewed;          47 AA.
AC   P0DV97;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Variabilin {ECO:0000303|PubMed:8663513};
DE   AltName: Full=Anti-platelet agent {ECO:0000303|PubMed:8663513};
DE   AltName: Full=Platelet aggregation inhibitor {ECO:0000305};
DE            Short=PAI {ECO:0000305};
OS   Dermacentor variabilis (American dog tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Rhipicephalinae; Dermacentor.
OX   NCBI_TaxID=34621;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Salivary gland;
RX   PubMed=8663513; DOI=10.1074/jbc.271.30.17785;
RA   Wang X., Coons L.B., Taylor D.B., Stevens S.E. Jr., Gartner T.K.;
RT   "Variabilin, a novel RGD-containing antagonist of glycoprotein IIb-IIIa and
RT   platelet aggregation inhibitor from the hard tick Dermacentor variabilis.";
RL   J. Biol. Chem. 271:17785-17790(1996).
CC   -!- FUNCTION: Potently inhibits platelet aggregation induced by ADP
CC       (IC(50)=157 nM, complete inhibition at 514 nM). Also inhibits platelet
CC       aggregation induced by collagen and by the thrombin receptor peptide
CC       SFLLRNP. Is a potent antagonist of the fibrinogen receptor glycoprotein
CC       IIb-IIIa (ITGA2B/ITGB3) and the vitronectin receptor alpha-v/beta-3
CC       (ITGAV/ITGB3). {ECO:0000269|PubMed:8663513}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:8663513}.
CC   -!- TISSUE SPECIFICITY: Expressed by salivary glands.
CC       {ECO:0000305|PubMed:8663513}.
CC   -!- PTM: Contains 2 disulfide bonds. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=4985.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:8663513};
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PE   1: Evidence at protein level;
KW   Cell adhesion impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Hemostasis impairing toxin; Platelet aggregation inhibiting toxin;
KW   Secreted; Toxin.
FT   CHAIN           1..47
FT                   /note="Variabilin"
FT                   /evidence="ECO:0000269|PubMed:8663513"
FT                   /id="PRO_0000455821"
FT   MOTIF           32..34
FT                   /note="Cell attachment site"
SQ   SEQUENCE   47 AA;  4973 MW;  0007869C78626647 CRC64;
     NTFSDENPGF PCDCTSADAK RACGIQCACW PRGDTPGGGR RIIDGQQ