ID   VARI_AMBVA              Reviewed;          32 AA.
AC   P85800;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Variegin;
OS   Amblyomma variegatum (Tropical bont tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Amblyomminae; Amblyomma.
OX   NCBI_TaxID=34610;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP   GLYCOSYLATION AT THR-14, AND VARIANT HIS-31.
RC   TISSUE=Salivary gland {ECO:0000269|PubMed:17684009};
RX   PubMed=17684009; DOI=10.1074/jbc.m705600200;
RA   Koh C.Y., Kazimirova M., Trimnell A., Takac P., Labuda M., Nuttall P.A.,
RA   Kini R.M.;
RT   "Variegin, a novel fast and tight binding thrombin inhibitor from the
RT   tropical bont tick.";
RL   J. Biol. Chem. 282:29101-29113(2007).
CC   -!- FUNCTION: Thrombin inhibitor. Does not inhibit other serine proteases.
CC       {ECO:0000269|PubMed:17684009}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17684009}.
CC   -!- MASS SPECTROMETRY: Mass=3769.96; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17684009};
CC   -!- MASS SPECTROMETRY: Mass=3776.79; Method=MALDI; Note=Variant His-31.;
CC       Evidence={ECO:0000269|PubMed:17684009};
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DR   PDB; 3B23; X-ray; 2.40 A; C=1-32.
DR   PDBsum; 3B23; -.
DR   AlphaFoldDB; P85800; -.
DR   SMR; P85800; -.
DR   MEROPS; I74.001; -.
DR   iPTMnet; P85800; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   DisProt; DP01833; -.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Glycoprotein; Protease inhibitor;
KW   Secreted; Serine protease inhibitor.
FT   PEPTIDE         1..32
FT                   /note="Variegin"
FT                   /evidence="ECO:0000269|PubMed:17684009"
FT                   /id="PRO_0000341476"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          8..14
FT                   /note="Contains the active site"
FT                   /evidence="ECO:0000269|PubMed:17684009"
FT   COMPBIAS        1..15
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        14
FT                   /note="O-linked (Hex) threonine"
FT                   /evidence="ECO:0000269|PubMed:17684009"
FT   VARIANT         31
FT                   /note="E -> H"
FT                   /evidence="ECO:0000269|PubMed:17684009"
SQ   SEQUENCE   32 AA;  3609 MW;  22448E8203FC70E2 CRC64;
     SDQGDVAEPK MHKTAPPFDF EAIPEEYLDD ES