VASA2_DROME
ID VASA2_DROME Reviewed; 1031 AA.
AC B6JUP5; Q4ABF3;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Sister chromatid cohesion protein solo {ECO:0000303|PubMed:20142422};
DE AltName: Full=Sisters on the loose {ECO:0000312|EMBL:ABI34808.1};
GN Name=solo {ECO:0000303|PubMed:20142422, ECO:0000312|FlyBase:FBgn0283440};
GN ORFNames=CG46282 {ECO:0000312|FlyBase:FBgn0283440};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:ABI34808.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC TISSUE=Testis {ECO:0000312|EMBL:ABI34808.1};
RX PubMed=20142422; DOI=10.1083/jcb.200904040;
RA Yan R., Thomas S.E., Tsai J.H., Yamada Y., McKee B.D.;
RT "SOLO: a meiotic protein required for centromere cohesion, coorientation,
RT and SMC1 localization in Drosophila melanogaster.";
RL J. Cell Biol. 188:335-349(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:ACV91646.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J.W., Booth B., Frise E., Sandler J., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22 AND THR-27, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo {ECO:0000269|PubMed:18327897};
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Isoform solo and SMC1 function as partners in mediating
CC sister chromatid and centromere cohesion in meiosis. Not required for
CC sister chromatid arm cohesion or for mitotic chromatid segregation.
CC {ECO:0000269|PubMed:20142422}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere
CC {ECO:0000269|PubMed:20142422}. Note=Colocalizes with SMC1 to meiotic
CC centromeres from early prophase I until both proteins disappear at
CC anaphase II. Ord is required for stable centromere localization.
CC {ECO:0000269|PubMed:20142422}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=solo {ECO:0000303|PubMed:20142422};
CC IsoId=B6JUP5-1; Sequence=Displayed;
CC Name=vas; Synonyms=A;
CC IsoId=P09052-1; Sequence=External;
CC -!- TISSUE SPECIFICITY: Expressed in spermatocytes.
CC {ECO:0000269|PubMed:20142422}.
CC -!- DISRUPTION PHENOTYPE: Premature loss of centromere cohesion and high
CC non-disjunction of both homologous and sister chromatids at meiosis I
CC and II. {ECO:0000269|PubMed:20142422}.
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DR EMBL; DQ851162; ABI34808.1; -; mRNA.
DR EMBL; AE014134; AAZ66470.2; -; Genomic_DNA.
DR EMBL; BT099809; ACV91646.1; -; mRNA.
DR RefSeq; NP_001027274.2; NM_001032103.2. [B6JUP5-1]
DR RefSeq; NP_001303323.1; NM_001316394.1. [B6JUP5-1]
DR AlphaFoldDB; B6JUP5; -.
DR SMR; B6JUP5; -.
DR BioGRID; 60902; 23.
DR STRING; 7227.FBpp0293395; -.
DR iPTMnet; B6JUP5; -.
DR PaxDb; B6JUP5; -.
DR DNASU; 26067079; -.
DR EnsemblMetazoa; FBtr0445188; FBpp0401448; FBgn0283440. [B6JUP5-1]
DR EnsemblMetazoa; FBtr0445189; FBpp0401449; FBgn0283440. [B6JUP5-1]
DR GeneID; 26067079; -.
DR KEGG; dme:Dmel_CG46282; -.
DR UCSC; CG33678-RA; d. melanogaster.
DR CTD; 26067079; -.
DR FlyBase; FBgn0283440; solo.
DR VEuPathDB; VectorBase:FBgn0283440; -.
DR eggNOG; KOG0335; Eukaryota.
DR HOGENOM; CLU_306145_0_0_1; -.
DR PhylomeDB; B6JUP5; -.
DR BioGRID-ORCS; 26067079; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 26067079; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0283440; Expressed in egg cell and 2 other tissues.
DR ExpressionAtlas; B6JUP5; baseline and differential.
DR Genevisible; B6JUP5; DM.
DR GO; GO:0000775; C:chromosome, centromeric region; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0051754; P:meiotic sister chromatid cohesion, centromeric; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Centromere; Chromosome; Developmental protein;
KW Differentiation; Meiosis; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..1031
FT /note="Sister chromatid cohesion protein solo"
FT /id="PRO_0000396515"
FT REPEAT 93..99
FT /note="1"
FT /evidence="ECO:0000255"
FT REPEAT 100..106
FT /note="2"
FT /evidence="ECO:0000255"
FT REPEAT 107..113
FT /note="3"
FT /evidence="ECO:0000255"
FT REPEAT 114..120
FT /note="4"
FT /evidence="ECO:0000255"
FT REPEAT 121..127
FT /note="5"
FT /evidence="ECO:0000255"
FT REGION 1..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 93..127
FT /note="5 X 7 AA tandem repeats of [FS]-R-G-G-[EQ]-G-G"
FT /evidence="ECO:0000255"
FT REGION 264..291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 323..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 623..661
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..32
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..97
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 264..285
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..344
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 638..652
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 22
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 27
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 1031 AA; 112515 MW; EA18ED8D0593C959 CRC64;
MSDDWDDEPI VDTRGARGGD WSDDEDTAKS FSGEAEGDGV GGSGGEGGGY QGGNRDVFGR
IGGGRGGGAG GYRGGNRDGG GFHGGRREGE RDFRGGEGGF RGGQGGSRGG QGGSRGGQGG
FRGGEGGFRG RLYENEDAKE LPPIPSSCDK DEVGAFIDQL DLSKYTTSLA LIRKLRDYLL
TAFKALVNQA WASLEKLAQE KRAQKAHQIR ETTDMMNRLM EDMGRLIREG EHEEANGIGM
GFQSIDADCR ADLSQWRNLQ QVVMPQSSEI RDGQTNRTSG TEGGRSGGLT DGDDLTEIQS
AAYAVYHQVL DRGYQTGRRL YDDNQGALRE DSIPTEDREH PENNQVDGST YGVTRFLHWL
SENFVTRHEI RAVRFGSPHM VFQSYDWPES PKQHISTVHQ KGIFYSQPLI RTRIIATVAS
EPNNSSSGQL SSMVNLARHQ RSQNQETPDG IYVSRYNATG DEETPCSATG VPGRVVALRA
SSTPNEHRSD PGLSFIPNLT YEDGTIPSLA AGGAASTPLA PSLPAEHSLG LEMDSNTLVV
PADADETRLV GHPGSTVVRS RLGGPPASTP LLSFRPRLPV IDEEHHLRFT RVSRELQMSV
AFPPEETTLS EQREWGSIFR PRDEMVNYAE GPPPRQTQRA RRRSRTRRRR NRIASAASPQ
LTPERIVRRE EAFQSSRHIA ATFMSSLLQN AAGEVTTSSG LAVTRSVKEI TTSVHITRNV
LQEEVPPTTE ATTTSLEVAQ ATGYSDSGAC MQSAAFSQDL PVVAPLEISG NASSDQASFD
QALALLPPIN STPIGQHPIA VFGPKTEIEI KPQKMDISNM MNTSVPPMPM EVDEVPLEVS
NTLANMSDHA YVTDIQILPS LQLNSLNLVS TNITDNAVNI APSTSTNDLQ TPSDRRTSMQ
NQLYRVSMND LAYISQHANV VRLMVDRQEE LGAQVSSLGP FKENSQGALN FSTMPAVKVY
DPRIIQNIQK DKMRVVHGLF GALIRQSQVD MHNAPFIRNR KDALMAYRFL LELKTANIIS
LSSDGRFFGL L
