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VASH2_MOUSE
ID   VASH2_MOUSE             Reviewed;         355 AA.
AC   Q8C5G2; Q3TR02; Q8BRA1; Q8R1R8;
DT   12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Tubulinyl-Tyr carboxypeptidase 2 {ECO:0000305};
DE            EC=3.4.17.17 {ECO:0000269|PubMed:29146868, ECO:0000269|PubMed:31324789};
DE   AltName: Full=Vasohibin-2 {ECO:0000303|PubMed:19204325};
DE   AltName: Full=Vasohibin-like protein;
GN   Name=Vash2 {ECO:0000312|MGI:MGI:2444826}; Synonyms=Vashl;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Cerebellum, Gonad, and Head;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=19204325; DOI=10.1182/blood-2008-07-170316;
RA   Kimura H., Miyashita H., Suzuki Y., Kobayashi M., Watanabe K., Sonoda H.,
RA   Ohta H., Fujiwara T., Shimosegawa T., Sato Y.;
RT   "Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2
RT   in the regulation of angiogenesis.";
RL   Blood 113:4810-4818(2009).
RN   [5]
RP   IDENTIFICATION AS A PROTEASE, AND ACTIVE SITES.
RX   PubMed=26794318; DOI=10.1093/bioinformatics/btv761;
RA   Sanchez-Pulido L., Ponting C.P.;
RT   "Vasohibins: new transglutaminase-like cysteine proteases possessing a non-
RT   canonical Cys-His-Ser catalytic triad.";
RL   Bioinformatics 32:1441-1445(2016).
RN   [6]
RP   FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH SVBP, ACTIVE SITE, AND
RP   MUTAGENESIS OF CYS-158.
RX   PubMed=29146868; DOI=10.1126/science.aao4165;
RA   Aillaud C., Bosc C., Peris L., Bosson A., Heemeryck P., Van Dijk J.,
RA   Le Friec J., Boulan B., Vossier F., Sanman L.E., Syed S., Amara N.,
RA   Coute Y., Lafanechere L., Denarier E., Delphin C., Pelletier L.,
RA   Humbert S., Bogyo M., Andrieux A., Rogowski K., Moutin M.J.;
RT   "Vasohibins/SVBP are tubulin carboxypeptidases (TCPs) that regulate neuron
RT   differentiation.";
RL   Science 358:1448-1453(2017).
RN   [7]
RP   FUNCTION.
RX   PubMed=31235911; DOI=10.1038/s41594-019-0241-y;
RA   Wang N., Bosc C., Ryul Choi S., Boulan B., Peris L., Olieric N., Bao H.,
RA   Krichen F., Chen L., Andrieux A., Olieric V., Moutin M.J., Steinmetz M.O.,
RA   Huang H.;
RT   "Structural basis of tubulin detyrosination by the vasohibin-SVBP enzyme
RT   complex.";
RL   Nat. Struct. Mol. Biol. 26:571-582(2019).
RN   [8] {ECO:0007744|PDB:6JZC, ECO:0007744|PDB:6JZD, ECO:0007744|PDB:6JZE}
RP   X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 48-301 IN COMPLEX WITH SVBP,
RP   INTERACTION WITH SVBP, FUNCTION, CATALYTIC ACTIVITY, AND MUTAGENESIS OF
RP   LYS-119; ARG-134; LYS-135; LYS-157; CYS-158; LYS-183; HIS-193; SER-210;
RP   ARG-211; ARG-212; LYS-218; LYS-238 AND LYS-247.
RX   PubMed=31324789; DOI=10.1038/s41467-019-11277-8;
RA   Zhou C., Yan L., Zhang W.H., Liu Z.;
RT   "Structural basis of tubulin detyrosination by VASH2/SVBP heterodimer.";
RL   Nat. Commun. 10:3212-3212(2019).
CC   -!- FUNCTION: Tyrosine carboxypeptidase that removes the C-terminal
CC       tyrosine residue of alpha-tubulin, thereby regulating microtubule
CC       dynamics and function (PubMed:29146868). Acts as an activator of
CC       angiogenesis: expressed in infiltrating mononuclear cells in the
CC       sprouting front to promote angiogenesis (PubMed:19204325). Plays a role
CC       in axon formation (PubMed:31235911). {ECO:0000269|PubMed:19204325,
CC       ECO:0000269|PubMed:29146868, ECO:0000269|PubMed:31235911}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-L-tyrosyl-
CC         [tubulin] + H2O = C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-
CC         [tubulin] + L-tyrosine; Xref=Rhea:RHEA:57444, Rhea:RHEA-COMP:16434,
CC         Rhea:RHEA-COMP:16435, ChEBI:CHEBI:15377, ChEBI:CHEBI:58315,
CC         ChEBI:CHEBI:149554, ChEBI:CHEBI:149555; EC=3.4.17.17;
CC         Evidence={ECO:0000269|PubMed:29146868, ECO:0000269|PubMed:31324789};
CC   -!- SUBUNIT: Interacts with SVBP; interaction enhances VASH2 tyrosine
CC       carboxypeptidase activity. {ECO:0000269|PubMed:29146868,
CC       ECO:0000269|PubMed:31324789}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q86V25}.
CC       Secreted {ECO:0000250|UniProtKB:Q86V25}. Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q86V25}. Note=Mainly localizes in the cytoplasm.
CC       Some fraction is secreted via a non-canonical secretion system;
CC       interaction with SVBP promotes secretion. Associates with microtubules
CC       (By similarity). {ECO:0000250|UniProtKB:Q86V25}.
CC   -!- TISSUE SPECIFICITY: Preferentially expressed in bone marrow-derived
CC       mononuclear cells at the sprouting front.
CC       {ECO:0000269|PubMed:29146868}.
CC   -!- SIMILARITY: Belongs to the transglutaminase-like superfamily. Vasohibin
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH24141.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AK045261; BAC32286.1; -; mRNA.
DR   EMBL; AK078596; BAC37339.1; -; mRNA.
DR   EMBL; AK140989; BAE24539.1; -; mRNA.
DR   EMBL; AK163193; BAE37229.1; -; mRNA.
DR   EMBL; CH466555; EDL13013.1; -; Genomic_DNA.
DR   EMBL; BC024141; AAH24141.1; ALT_INIT; mRNA.
DR   CCDS; CCDS48482.1; -.
DR   RefSeq; NP_659128.2; NM_144879.2.
DR   PDB; 6JZC; X-ray; 2.20 A; A/B=1-355.
DR   PDB; 6JZD; X-ray; 2.48 A; A=1-355.
DR   PDB; 6JZE; X-ray; 2.51 A; A=47-355.
DR   PDBsum; 6JZC; -.
DR   PDBsum; 6JZD; -.
DR   PDBsum; 6JZE; -.
DR   AlphaFoldDB; Q8C5G2; -.
DR   SMR; Q8C5G2; -.
DR   STRING; 10090.ENSMUSP00000036768; -.
DR   iPTMnet; Q8C5G2; -.
DR   PhosphoSitePlus; Q8C5G2; -.
DR   PaxDb; Q8C5G2; -.
DR   PRIDE; Q8C5G2; -.
DR   ProteomicsDB; 297910; -.
DR   Antibodypedia; 47116; 152 antibodies from 22 providers.
DR   DNASU; 226841; -.
DR   Ensembl; ENSMUST00000047409; ENSMUSP00000036768; ENSMUSG00000037568.
DR   Ensembl; ENSMUST00000166139; ENSMUSP00000127626; ENSMUSG00000037568.
DR   GeneID; 226841; -.
DR   KEGG; mmu:226841; -.
DR   UCSC; uc007ebr.2; mouse.
DR   CTD; 79805; -.
DR   MGI; MGI:2444826; Vash2.
DR   VEuPathDB; HostDB:ENSMUSG00000037568; -.
DR   eggNOG; ENOG502QPPX; Eukaryota.
DR   GeneTree; ENSGT00390000012703; -.
DR   HOGENOM; CLU_061405_0_0_1; -.
DR   InParanoid; Q8C5G2; -.
DR   OMA; WGHVERV; -.
DR   OrthoDB; 908291at2759; -.
DR   PhylomeDB; Q8C5G2; -.
DR   TreeFam; TF329370; -.
DR   BioGRID-ORCS; 226841; 1 hit in 72 CRISPR screens.
DR   PRO; PR:Q8C5G2; -.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; Q8C5G2; protein.
DR   Bgee; ENSMUSG00000037568; Expressed in humerus cartilage element and 155 other tissues.
DR   Genevisible; Q8C5G2; MM.
DR   GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; IDA:UniProtKB.
DR   GO; GO:0004181; F:metallocarboxypeptidase activity; IDA:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0061564; P:axon development; ISS:UniProtKB.
DR   GO; GO:0140253; P:cell-cell fusion; ISO:MGI.
DR   GO; GO:0060716; P:labyrinthine layer blood vessel development; IMP:MGI.
DR   GO; GO:0060711; P:labyrinthine layer development; IMP:MGI.
DR   GO; GO:0060674; P:placenta blood vessel development; IMP:MGI.
DR   GO; GO:0045766; P:positive regulation of angiogenesis; IMP:MGI.
DR   GO; GO:0001938; P:positive regulation of endothelial cell proliferation; ISO:MGI.
DR   GO; GO:0006508; P:proteolysis; IDA:UniProtKB.
DR   GO; GO:0045765; P:regulation of angiogenesis; IBA:GO_Central.
DR   GO; GO:0000768; P:syncytium formation by plasma membrane fusion; IMP:MGI.
DR   InterPro; IPR028131; VASH1.
DR   PANTHER; PTHR15750; PTHR15750; 1.
DR   Pfam; PF14822; Vasohibin; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Carboxypeptidase; Cytoplasm; Cytoskeleton; Hydrolase;
KW   Phosphoprotein; Protease; Reference proteome; Secreted.
FT   CHAIN           1..355
FT                   /note="Tubulinyl-Tyr carboxypeptidase 2"
FT                   /id="PRO_0000189983"
FT   REGION          1..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          297..335
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        158
FT                   /evidence="ECO:0000269|PubMed:29146868,
FT                   ECO:0000305|PubMed:26794318"
FT   ACT_SITE        193
FT                   /evidence="ECO:0000305|PubMed:26794318"
FT   ACT_SITE        210
FT                   /evidence="ECO:0000305|PubMed:26794318"
FT   MOD_RES         302
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q86V25"
FT   MUTAGEN         119
FT                   /note="K->E: No effect on tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         134
FT                   /note="R->E: Slightly reduced tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         135
FT                   /note="K->E: Strongly reduced tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         157
FT                   /note="K->E: Strongly reduced tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         158
FT                   /note="C->A: Abolished tyrosine carboxypeptidase activity."
FT                   /evidence="ECO:0000269|PubMed:29146868,
FT                   ECO:0000269|PubMed:31324789"
FT   MUTAGEN         183
FT                   /note="K->E: No effect on tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         193
FT                   /note="H->A: Strongly reduced tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         210
FT                   /note="S->A: Strongly reduced tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         211
FT                   /note="R->E: Strongly reduced tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         212
FT                   /note="R->E: Strongly reduced tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         218
FT                   /note="K->E: Strongly reduced tyrosine carboxypeptidase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         238
FT                   /note="K->E: Reduced tyrosine carboxypeptidase activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   MUTAGEN         247
FT                   /note="K->E: Reduced tyrosine carboxypeptidase activity."
FT                   /evidence="ECO:0000269|PubMed:31324789"
FT   CONFLICT        341
FT                   /note="A -> D (in Ref. 1; BAC37339)"
FT                   /evidence="ECO:0000305"
FT   HELIX           60..73
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   HELIX           77..84
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   HELIX           107..121
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   STRAND          126..131
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   HELIX           139..152
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   HELIX           158..169
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   STRAND          176..186
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   STRAND          189..200
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   STRAND          203..207
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   HELIX           213..215
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   STRAND          218..224
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   HELIX           225..237
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   TURN            238..240
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   STRAND          242..248
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   STRAND          266..269
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   HELIX           271..273
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   HELIX           276..291
FT                   /evidence="ECO:0007829|PDB:6JZC"
FT   TURN            298..300
FT                   /evidence="ECO:0007829|PDB:6JZE"
SQ   SEQUENCE   355 AA;  40499 MW;  74B25608F2297D14 CRC64;
     MTGSAADTHR CPHPKITKGT RSRSSHARPV SLATSGGSEE EDKDGGVLFH VNKSGFPIDS
     HTWERMWLHV AKVHPRGGEM VGAIRNAAFL AKPSIPQVPN YRLSMTIPDW LQAIQNYMKT
     LQYNHTGTQF FEIRKMRPLS GLMETAKEMT RESLPIKCLE AVILGIYLTN GQPSIERFPI
     SFKTYFSGNY FHHVVLGIYC NGYYGSLGMS RRAELMDKPL TFRTLSDLVF DFEDSYKKYL
     HTVKKVKIGL YVPHEPHSFQ PIEWKQLVLN VSKMLRADIR KELEKYARDM RMKILKPASA
     HSPTQVRSRG KSLSPRRRQA SPPRRLGRRD KSPALTEKKV ADLGTLNEVG YQIRI
 
 
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