ID   VASH_VIBCH              Reviewed;         530 AA.
AC   Q9KN48;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Transcriptional regulator VasH {ECO:0000303|PubMed:21949076};
GN   Name=vasH {ECO:0000303|PubMed:21949076}; OrderedLocusNames=VC_A0117;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   FUNCTION.
RX   PubMed=21949076; DOI=10.1128/jb.05414-11;
RA   Kitaoka M., Miyata S.T., Brooks T.M., Unterweger D., Pukatzki S.;
RT   "VasH is a transcriptional regulator of the type VI secretion system
RT   functional in endemic and pandemic Vibrio cholerae.";
RL   J. Bacteriol. 193:6471-6482(2011).
CC   -!- FUNCTION: Transcriptional regulator of the type VI secretion system.
CC       {ECO:0000269|PubMed:21949076}.
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DR   EMBL; AE003853; AAF96031.1; -; Genomic_DNA.
DR   PIR; H82499; H82499.
DR   RefSeq; NP_232518.1; NC_002506.1.
DR   RefSeq; WP_000084793.1; NZ_LT906615.1.
DR   AlphaFoldDB; Q9KN48; -.
DR   SMR; Q9KN48; -.
DR   STRING; 243277.VC_A0117; -.
DR   TCDB; 3.A.23.1.1; the type vi symbiosis/virulence secretory system (t6ss) family.
DR   PRIDE; Q9KN48; -.
DR   DNASU; 2612409; -.
DR   EnsemblBacteria; AAF96031; AAF96031; VC_A0117.
DR   GeneID; 57741579; -.
DR   KEGG; vch:VC_A0117; -.
DR   PATRIC; fig|243277.26.peg.2758; -.
DR   eggNOG; COG3829; Bacteria.
DR   HOGENOM; CLU_000445_95_4_6; -.
DR   OMA; VACAHTP; -.
DR   BioCyc; VCHO:VCA0117-MON; -.
DR   Proteomes; UP000000584; Chromosome 2.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   ATP-binding; DNA-binding; Nucleotide-binding; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..530
FT                   /note="Transcriptional regulator VasH"
FT                   /id="PRO_0000449211"
FT   DOMAIN          193..422
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         221..228
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         284..293
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   530 AA;  59260 MW;  6E8027DBCFC42904 CRC64;
     MSQWLAFATQ LVGVRKSHQL ALQFVDLLTQ GLDLSDSLLL LPSSDGRLLV PHDPQRQFAW
     SVTDFDVPFA HVLQSSNAMH LTAEELVFWQ SNRTFSQLTS RVGMFDSVWI QPLPMDTRQV
     HSILLLMGES HGIVSAFENA DFLKFIEVFS QQWSLLNDME REEQRRLELK QSLTDIERDS
     AQRSLANALS RTLIGESAAM QKLREQIVSA ANSQLSVMVQ GETGTGKELV AAAVHELSSR
     KSAPFVAINC AAIPEHLLES ELFGYCKGAF SGADSDKQGL IAQANGGTLF LDEIGDMPLT
     LQAKLLRVLE SRTFRPLGGK QELSSDFRLV SATHVNLLDQ VRKKEFRQDL YYRLFQYPIT
     LPRLAARLED IELLSEHFVR VFNLQHNTRI RGLNYRAIDC LKQYDFPGNV RELKHLIEFG
     CAQTADGTQV EASCFAHRLQ TLPCLAPEAT PVAVSVETEN VDLEPSVALA GEPNFAVIHD
     LKQAVSQFEA LIISERLNRF AGDRAKAAKS LGIPKRTLAY KCLKLEIKTP