ID   VASO1_TABYA             Reviewed;          76 AA.
AC   C1IBZ2;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 16.
DE   RecName: Full=Vasotab-TY1 {ECO:0000303|PubMed:19531497};
DE   AltName: Full=Vasotab TY {ECO:0000303|PubMed:18087067};
DE   Flags: Precursor;
OS   Tabanus yao (Horsefly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Tabanomorpha; Tabanoidea;
OC   Tabanidae; Tabanus.
OX   NCBI_TaxID=485572 {ECO:0000312|EMBL:ABX80080.1};
RN   [1] {ECO:0000312|EMBL:ABX80080.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SYNTHESIS.
RC   TISSUE=Salivary gland;
RX   PubMed=18087067; DOI=10.1074/mcp.m700497-mcp200;
RA   Xu X., Yang H., Ma D., Wu J., Wang Y., Song Y., Wang X., Lu Y., Yang J.,
RA   Lai R.;
RT   "Toward an understanding of the molecular mechanism for successful blood
RT   feeding by coupling proteomics analysis with pharmacological testing of
RT   horsefly salivary glands.";
RL   Mol. Cell. Proteomics 7:582-590(2008).
RN   [2]
RP   PURIFICATION.
RC   TISSUE=Salivary gland;
RX   PubMed=19531497; DOI=10.1074/mcp.m900186-mcp200;
RA   Ma D., Wang Y., Yang H., Wu J., An S., Gao L., Xu X., Lai R.;
RT   "Anti-thrombosis repertoire of blood-feeding horsefly salivary glands.";
RL   Mol. Cell. Proteomics 8:2071-2079(2009).
CC   -!- FUNCTION: Vasodilator protein that inhibits vasoconstriction of
CC       isolated rat femoral artery induced by phenylephrine (PubMed:18087067).
CC       Since platelet aggregation and vasoconstriction are key hemostatic
CC       responses, particularly in small wounds, this protein likely
CC       participates in the antihemostatic responses during blood feeding (By
CC       similarity). Blocks L-type calcium channels (Cav1/CACNA1) in left
CC       ventricular myocytes isolated from rat hearts (By similarity).
CC       {ECO:0000250|UniProtKB:P84843, ECO:0000269|PubMed:18087067}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P84843}.
CC   -!- TISSUE SPECIFICITY: Expressed by the salivary gland.
CC       {ECO:0000250|UniProtKB:P84843}.
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DR   EMBL; EU147263; ABX80080.1; -; mRNA.
DR   InterPro; IPR002350; Kazal_dom.
DR   InterPro; IPR036058; Kazal_dom_sf.
DR   Pfam; PF00050; Kazal_1; 1.
DR   SMART; SM00280; KAZAL; 1.
DR   SUPFAM; SSF100895; SSF100895; 1.
DR   PROSITE; PS51465; KAZAL_2; 1.
PE   3: Inferred from homology;
KW   Calcium channel impairing toxin; Disulfide bond;
KW   Ion channel impairing toxin; Secreted; Signal; Toxin; Vasoactive;
KW   Vasodilator; Voltage-gated calcium channel impairing toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000250|UniProtKB:C8YJB3,
FT                   ECO:0000250|UniProtKB:C8YJB4"
FT   CHAIN           22..76
FT                   /note="Vasotab-TY1"
FT                   /evidence="ECO:0000305|PubMed:18087067"
FT                   /id="PRO_5002910666"
FT   DOMAIN          22..76
FT                   /note="Kazal-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        23..60
FT                   /evidence="ECO:0000250|UniProtKB:P84843,
FT                   ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        27..53
FT                   /evidence="ECO:0000250|UniProtKB:P84843,
FT                   ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        35..75
FT                   /evidence="ECO:0000250|UniProtKB:P84843,
FT                   ECO:0000255|PROSITE-ProRule:PRU00798"
SQ   SEQUENCE   76 AA;  8299 MW;  65043EC701B2C4F8 CRC64;
     MKFTLFSVLV VLLIATFVAA DDCPRICTAD FRPVCGTPSG GRRSANRTFG NQCSLDSHNC
     LNKGDTYDKL HDGECK