ID   VASO2_TABYA             Reviewed;          76 AA.
AC   C8YJB3;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 1.
DT   03-AUG-2022, entry version 16.
DE   RecName: Full=Vasotab-TY2 {ECO:0000303|PubMed:19531497};
DE   Flags: Precursor;
OS   Tabanus yao (Horsefly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Tabanomorpha; Tabanoidea;
OC   Tabanidae; Tabanus.
OX   NCBI_TaxID=485572;
RN   [1] {ECO:0000312|EMBL:ACS72308.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-41, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Salivary gland;
RX   PubMed=19531497; DOI=10.1074/mcp.m900186-mcp200;
RA   Ma D., Wang Y., Yang H., Wu J., An S., Gao L., Xu X., Lai R.;
RT   "Anti-thrombosis repertoire of blood-feeding horsefly salivary glands.";
RL   Mol. Cell. Proteomics 8:2071-2079(2009).
CC   -!- FUNCTION: Vasodilator protein that inhibits vasoconstriction of
CC       isolated rat femoral artery induced by phenylephrine (PubMed:19531497).
CC       Since platelet aggregation and vasoconstriction are key hemostatic
CC       responses, particularly in small wounds, this protein likely
CC       participates in the antihemostatic responses during blood feeding
CC       (PubMed:19531497). Blocks L-type calcium channels (Cav1/CACNA1) in left
CC       ventricular myocytes isolated from rat hearts (By similarity).
CC       {ECO:0000250|UniProtKB:P84843, ECO:0000269|PubMed:19531497}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19531497}.
CC   -!- TISSUE SPECIFICITY: Expressed by the salivary gland.
CC       {ECO:0000269|PubMed:19531497}.
CC   -!- MASS SPECTROMETRY: Mass=5914.4; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19531497};
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DR   EMBL; FJ469620; ACS72308.1; -; mRNA.
DR   InterPro; IPR002350; Kazal_dom.
DR   InterPro; IPR036058; Kazal_dom_sf.
DR   Pfam; PF00050; Kazal_1; 1.
DR   SMART; SM00280; KAZAL; 1.
DR   SUPFAM; SSF100895; SSF100895; 1.
DR   PROSITE; PS51465; KAZAL_2; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Secreted; Signal; Toxin; Vasoactive;
KW   Vasodilator; Voltage-gated calcium channel impairing toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000305|PubMed:19531497"
FT   CHAIN           22..76
FT                   /note="Vasotab-TY2"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_5002994499"
FT   DOMAIN          22..76
FT                   /note="Kazal-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        23..60
FT                   /evidence="ECO:0000250|UniProtKB:P84843,
FT                   ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        27..53
FT                   /evidence="ECO:0000250|UniProtKB:P84843,
FT                   ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        35..75
FT                   /evidence="ECO:0000250|UniProtKB:P84843,
FT                   ECO:0000255|PROSITE-ProRule:PRU00798"
SQ   SEQUENCE   76 AA;  8202 MW;  01617C52840888FE CRC64;
     MKFALFSVLV LMLIATFVAA DDCPRICTAD YTPVCGTPSG GRRSANRTFA NQCGLDSHNC
     LNKGATYDKL HDGECK