ID   CAHC_TOBAC              Reviewed;         321 AA.
AC   P27141;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Carbonic anhydrase, chloroplastic;
DE            EC=4.2.1.1;
DE   AltName: Full=Carbonate dehydratase;
DE   Flags: Precursor;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Majeau N., Coleman J.R.;
RT   "Isolation of carbonic anhydrase cDNA from tobacco.";
RL   Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 58-321.
RC   STRAIN=cv. SR1; TISSUE=Leaf;
RA   Price G.D., Harrison K., Gallagher A., Badger M.R.;
RT   "Isolation of a cDNA clone for tobacco chloroplastic carbonic anhydrase.";
RL   Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Reversible hydration of carbon dioxide.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + hydrogencarbonate = CO2 + H2O; Xref=Rhea:RHEA:10748,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:17544; EC=4.2.1.1;
CC   -!- SUBUNIT: Homohexamer.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC   -!- SIMILARITY: Belongs to the beta-class carbonic anhydrase family.
CC       {ECO:0000305}.
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DR   EMBL; M94135; AAA34065.1; -; mRNA.
DR   EMBL; L19255; AAA34057.1; -; mRNA.
DR   PIR; T02886; T02886.
DR   PIR; T02936; T02936.
DR   RefSeq; NP_001313031.1; NM_001326102.1.
DR   AlphaFoldDB; P27141; -.
DR   SMR; P27141; -.
DR   STRING; 4097.P27141; -.
DR   PRIDE; P27141; -.
DR   GeneID; 107822687; -.
DR   KEGG; nta:107822687; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0004089; F:carbonate dehydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0015976; P:carbon utilization; IEA:InterPro.
DR   CDD; cd00884; beta_CA_cladeB; 1.
DR   Gene3D; 3.40.1050.10; -; 1.
DR   InterPro; IPR045066; Beta_CA_cladeB.
DR   InterPro; IPR001765; Carbonic_anhydrase.
DR   InterPro; IPR015892; Carbonic_anhydrase_CS.
DR   InterPro; IPR036874; Carbonic_anhydrase_sf.
DR   PANTHER; PTHR11002; PTHR11002; 1.
DR   Pfam; PF00484; Pro_CA; 1.
DR   SMART; SM00947; Pro_CA; 1.
DR   SUPFAM; SSF53056; SSF53056; 1.
DR   PROSITE; PS00704; PROK_CO2_ANHYDRASE_1; 1.
DR   PROSITE; PS00705; PROK_CO2_ANHYDRASE_2; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Lyase; Plastid; Reference proteome; Transit peptide; Zinc.
FT   TRANSIT         1..100
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           101..321
FT                   /note="Carbonic anhydrase, chloroplastic"
FT                   /id="PRO_0000004272"
FT   CONFLICT        180
FT                   /note="V -> I (in Ref. 2; AAA34057)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        259
FT                   /note="A -> G (in Ref. 2; AAA34057)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   321 AA;  34511 MW;  AD88D86616C6A296 CRC64;
     MSTASINSCL TISPAQASLK KPTRPVAFAR LSNSSSSTSV PSLIRNEPVF AAPTPIINPI
     LREEMAKESY EQAIAALEKL LSEKGELGPI AAARVDQITA ELQSSDGSKP FDPVEHMKAG
     FIHFKTEKYE KNPALYGELS KGQSPKFMVF ACSDSRVCPS HVLNFQPGEA FVVRNIANMV
     PAYDKTRYSG VGAAIEYAVL HLKVENIVVI GHSACGGIKG LMSLPADGSE STAFIEDWVK
     IGLPAKAKVQ GEHVDKCFAD QCTACEKEAV NVSLGNLLTY PFVREGLVKK TLALKGGHYD
     FVNGGFELWG LEFGLSPSLS V